Pseudoalteromonas sp. NBT06-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas; unclassified Pseudoalteromonas

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4921 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A269PT70|A0A269PT70_9GAMM Porin OS=Pseudoalteromonas sp. NBT06-2 OX=2025950 GN=CJF42_04650 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.14FQKK5 pKa = 9.46TLLACTVSVFLMGCISDD22 pKa = 4.45NDD24 pKa = 4.27DD25 pKa = 3.55PSKK28 pKa = 11.19DD29 pKa = 3.87LIVPEE34 pKa = 4.18PVSVKK39 pKa = 10.48LVVPAEE45 pKa = 4.23GKK47 pKa = 11.05SMVEE51 pKa = 4.03DD52 pKa = 5.13FPLGMYY58 pKa = 7.66VTYY61 pKa = 10.36PEE63 pKa = 4.21SEE65 pKa = 3.98QRR67 pKa = 11.84ASAITSIDD75 pKa = 3.75VNWPFVDD82 pKa = 4.05VFQSGVEE89 pKa = 3.82YY90 pKa = 10.53GYY92 pKa = 10.47IDD94 pKa = 4.22GDD96 pKa = 3.78SDD98 pKa = 3.52EE99 pKa = 5.54LFYY102 pKa = 11.29HH103 pKa = 6.37SVEE106 pKa = 4.15NTNDD110 pKa = 3.1IAKK113 pKa = 10.21NAIDD117 pKa = 5.08LEE119 pKa = 4.19RR120 pKa = 11.84QEE122 pKa = 5.69DD123 pKa = 3.87ILWYY127 pKa = 9.12VTGNNLLFRR136 pKa = 11.84YY137 pKa = 9.86DD138 pKa = 3.5RR139 pKa = 11.84AQDD142 pKa = 4.28KK143 pKa = 10.79LVTWQVTGNSVFTEE157 pKa = 4.01LAIDD161 pKa = 3.7EE162 pKa = 4.87KK163 pKa = 11.42GEE165 pKa = 4.05QQVWLYY171 pKa = 11.54DD172 pKa = 3.66KK173 pKa = 10.05VTHH176 pKa = 6.17QLVIFNAIDD185 pKa = 3.51EE186 pKa = 4.44SSQVIPLEE194 pKa = 4.03GDD196 pKa = 3.29MSVEE200 pKa = 3.97GLGISEE206 pKa = 4.88DD207 pKa = 3.68SFLILAKK214 pKa = 10.47DD215 pKa = 3.66VAQGVVLHH223 pKa = 6.04YY224 pKa = 10.85SLAGTTLAYY233 pKa = 10.45NDD235 pKa = 2.92SWYY238 pKa = 11.26LEE240 pKa = 4.28GFGAAQFNDD249 pKa = 3.53IGLMPDD255 pKa = 2.9GRR257 pKa = 11.84IAVSNDD263 pKa = 2.84DD264 pKa = 3.85TEE266 pKa = 4.68NNLVFVMDD274 pKa = 4.37KK275 pKa = 9.61MAQIGAGPIKK285 pKa = 10.83DD286 pKa = 3.45NGEE289 pKa = 3.83LEE291 pKa = 4.63KK292 pKa = 10.62ISEE295 pKa = 4.24HH296 pKa = 5.96TLDD299 pKa = 3.85EE300 pKa = 4.96VIIQPSGIWALTDD313 pKa = 4.04DD314 pKa = 4.6SWMMITDD321 pKa = 3.61QAEE324 pKa = 4.28MFALDD329 pKa = 3.44ANFNVTEE336 pKa = 4.17KK337 pKa = 11.02VDD339 pKa = 3.89IEE341 pKa = 4.18FDD343 pKa = 4.92SINCNQGCTEE353 pKa = 4.99AIVGGDD359 pKa = 3.4NEE361 pKa = 5.11FYY363 pKa = 11.25ALTDD367 pKa = 3.54GGLVGHH373 pKa = 5.99FTKK376 pKa = 10.44TDD378 pKa = 3.15EE379 pKa = 4.55SYY381 pKa = 10.48TLAKK385 pKa = 9.88EE386 pKa = 3.94YY387 pKa = 10.53QINVTNSEE395 pKa = 4.23GEE397 pKa = 3.95NYY399 pKa = 9.93RR400 pKa = 11.84YY401 pKa = 10.26SGLGYY406 pKa = 10.27DD407 pKa = 4.12VSAQEE412 pKa = 4.61YY413 pKa = 10.61YY414 pKa = 10.91LIPDD418 pKa = 3.89QNDD421 pKa = 2.86EE422 pKa = 4.35DD423 pKa = 4.16ATDD426 pKa = 3.72EE427 pKa = 5.21LIILNSDD434 pKa = 3.72FTLKK438 pKa = 10.15EE439 pKa = 4.08RR440 pKa = 11.84YY441 pKa = 9.3DD442 pKa = 3.16ISYY445 pKa = 10.87AGEE448 pKa = 3.95TDD450 pKa = 3.58GSIFEE455 pKa = 4.39YY456 pKa = 10.52DD457 pKa = 3.34AQGVQYY463 pKa = 9.73HH464 pKa = 5.16QNNVYY469 pKa = 10.29VLSEE473 pKa = 4.45KK474 pKa = 9.31FTKK477 pKa = 10.59LIKK480 pKa = 10.62LNLVGEE486 pKa = 4.65IIAVYY491 pKa = 10.44DD492 pKa = 4.27LGHH495 pKa = 7.05EE496 pKa = 4.24DD497 pKa = 4.04VKK499 pKa = 11.35DD500 pKa = 3.8PSDD503 pKa = 3.3IALKK507 pKa = 10.63DD508 pKa = 3.37GQVYY512 pKa = 10.5VLGDD516 pKa = 3.79HH517 pKa = 6.89EE518 pKa = 4.95NDD520 pKa = 3.42EE521 pKa = 4.66PVPPLSVFDD530 pKa = 3.63IVEE533 pKa = 4.12YY534 pKa = 10.44EE535 pKa = 3.82

Molecular weight: 59.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A269PUH6|A0A269PUH6_9GAMM Bifunctional protein PutA OS=Pseudoalteromonas sp. NBT06-2 OX=2025950 GN=putA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.36RR14 pKa = 11.84SHH16 pKa = 6.15GFRR19 pKa = 11.84ARR21 pKa = 11.84MATTNGRR28 pKa = 11.84NLINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4921

0

4921

1573380

30

3199

319.7

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.298 ± 0.03

1.03 ± 0.015

5.523 ± 0.03

5.937 ± 0.029

4.506 ± 0.022

6.101 ± 0.036

2.143 ± 0.017

7.588 ± 0.029

6.824 ± 0.038

10.304 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.018

5.615 ± 0.033

3.402 ± 0.019

4.454 ± 0.029

3.606 ± 0.026

7.172 ± 0.029

5.529 ± 0.028

6.168 ± 0.029

1.144 ± 0.014

3.339 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski