Pelagimonas varians

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Pelagimonas

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A238KR60|A0A238KR60_9RHOB RNA polymerase sigma factor RpoH OS=Pelagimonas varians OX=696760 GN=rpoH_1 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84NIMLSLGFSLMPLAAFAQAQVDD24 pKa = 3.68ASDD27 pKa = 3.83PAVLAGLLQAEE38 pKa = 5.74GYY40 pKa = 7.58TLEE43 pKa = 4.17ITTDD47 pKa = 3.37AVGDD51 pKa = 3.86PLLKK55 pKa = 10.65GKK57 pKa = 10.46LDD59 pKa = 3.48GTSYY63 pKa = 11.21DD64 pKa = 3.29IYY66 pKa = 10.88FYY68 pKa = 11.14DD69 pKa = 3.67CTNNTEE75 pKa = 3.95CRR77 pKa = 11.84TIQFQVGYY85 pKa = 10.83DD86 pKa = 3.48MTNGMSLARR95 pKa = 11.84ANQWNSDD102 pKa = 3.14MRR104 pKa = 11.84YY105 pKa = 9.48AAAHH109 pKa = 6.86LDD111 pKa = 3.68EE112 pKa = 5.39EE113 pKa = 4.6MDD115 pKa = 4.3PFLQMDD121 pKa = 3.67MNIDD125 pKa = 3.68YY126 pKa = 10.61GVSAEE131 pKa = 4.03NFVDD135 pKa = 4.19NFKK138 pKa = 9.62MWTNVLGQFEE148 pKa = 5.7DD149 pKa = 5.15FIDD152 pKa = 3.6WDD154 pKa = 3.68

Molecular weight: 17.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238K510|A0A238K510_9RHOB Glucose--fructose oxidoreductase OS=Pelagimonas varians OX=696760 GN=gfo_2 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4735

0

4735

1443737

29

4058

304.9

33.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.441 ± 0.044

0.943 ± 0.013

6.174 ± 0.039

5.636 ± 0.036

3.884 ± 0.024

8.587 ± 0.04

2.061 ± 0.019

5.37 ± 0.027

3.604 ± 0.028

9.836 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.869 ± 0.022

2.96 ± 0.022

4.867 ± 0.029

3.511 ± 0.022

5.938 ± 0.039

5.734 ± 0.03

5.643 ± 0.034

7.292 ± 0.031

1.392 ± 0.015

2.258 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski