Aeribacillus phage AP45

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kamchatkavirus; Aeribacillus virus AP45

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2JY64|A0A1L2JY64_9CAUD Baseplate protein OS=Aeribacillus phage AP45 OX=1913112 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84VIEE5 pKa = 4.23IEE7 pKa = 4.15KK8 pKa = 10.53EE9 pKa = 3.87NLPEE13 pKa = 4.76IFDD16 pKa = 3.79IEE18 pKa = 4.52LGVEE22 pKa = 4.58LFTLEE27 pKa = 3.73IDD29 pKa = 3.69YY30 pKa = 11.59NEE32 pKa = 4.77AGDD35 pKa = 4.42FFTVDD40 pKa = 4.08LYY42 pKa = 11.3QYY44 pKa = 10.55DD45 pKa = 3.94NPDD48 pKa = 3.82PLVLGEE54 pKa = 5.42KK55 pKa = 10.69LMLNQPLFRR64 pKa = 11.84DD65 pKa = 3.55IEE67 pKa = 4.5DD68 pKa = 3.67GRR70 pKa = 11.84FPAPTLIPVDD80 pKa = 3.78PSGKK84 pKa = 9.11EE85 pKa = 4.16SRR87 pKa = 11.84ITWEE91 pKa = 3.92NFGVTVFLVVDD102 pKa = 4.63DD103 pKa = 5.19GDD105 pKa = 4.22DD106 pKa = 3.61NEE108 pKa = 4.33

Molecular weight: 12.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2JY24|A0A1L2JY24_9CAUD Uncharacterized protein OS=Aeribacillus phage AP45 OX=1913112 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.28GYY4 pKa = 10.49LRR6 pKa = 11.84AEE8 pKa = 4.32RR9 pKa = 11.84KK10 pKa = 9.96DD11 pKa = 3.24WMNDD15 pKa = 2.83GLATFHH21 pKa = 6.54LGIWRR26 pKa = 11.84FIIRR30 pKa = 11.84DD31 pKa = 3.21RR32 pKa = 11.84WILDD36 pKa = 3.07RR37 pKa = 11.84LVFNRR42 pKa = 11.84KK43 pKa = 8.42IKK45 pKa = 10.0KK46 pKa = 8.54QQAKK50 pKa = 9.9AYY52 pKa = 8.97CKK54 pKa = 10.49YY55 pKa = 10.93SLLSLYY61 pKa = 10.54DD62 pKa = 3.34RR63 pKa = 11.84AFAPVNMVGKK73 pKa = 10.17KK74 pKa = 10.05NPLFRR79 pKa = 11.84TSPTPHH85 pKa = 6.74FLAILMRR92 pKa = 11.84ATHH95 pKa = 5.66QKK97 pKa = 10.12GVNN100 pKa = 3.26

Molecular weight: 11.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

15689

54

1251

214.9

24.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.285 ± 0.418

0.688 ± 0.102

5.743 ± 0.256

8.076 ± 0.557

4.455 ± 0.217

6.042 ± 0.288

1.562 ± 0.123

7.61 ± 0.265

8.987 ± 0.38

8.222 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.17

5.934 ± 0.275

3.079 ± 0.172

4.028 ± 0.238

4.57 ± 0.236

5.985 ± 0.336

5.086 ± 0.245

6.176 ± 0.264

1.249 ± 0.122

3.665 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski