Pleurocapsa sp. CCALA 161

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Pleurocapsales; Hyellaceae; Pleurocapsa; unclassified Pleurocapsa

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T1CQ04|A0A2T1CQ04_9CYAN Antitoxin OS=Pleurocapsa sp. CCALA 161 OX=2107688 GN=C7B62_09835 PE=3 SV=1
MM1 pKa = 7.73AFNTTSTIAEE11 pKa = 4.29DD12 pKa = 3.26WNGGYY17 pKa = 10.39KK18 pKa = 10.31LEE20 pKa = 4.31LAISNDD26 pKa = 3.07LGAADD31 pKa = 3.31WVLNFNLPYY40 pKa = 9.71TISAAYY46 pKa = 9.32GVNLTQNSDD55 pKa = 2.69GSYY58 pKa = 9.62TIKK61 pKa = 10.98GQDD64 pKa = 3.02GWTSLTPGQTIKK76 pKa = 10.72PIFIIEE82 pKa = 4.25DD83 pKa = 3.4QGQSAQLLNFVDD95 pKa = 4.19QQQSNPIATVDD106 pKa = 3.52DD107 pKa = 4.61FEE109 pKa = 7.34NIDD112 pKa = 3.64NSYY115 pKa = 10.72LPSGNNTIDD124 pKa = 3.04VDD126 pKa = 3.97RR127 pKa = 11.84DD128 pKa = 3.68FGGDD132 pKa = 3.29LATAIAYY139 pKa = 8.63ANNGDD144 pKa = 4.03VVQLGNNRR152 pKa = 11.84YY153 pKa = 6.29YY154 pKa = 10.88TSGITIDD161 pKa = 3.99KK162 pKa = 10.79NIILDD167 pKa = 3.86GQEE170 pKa = 4.27DD171 pKa = 4.45SVIDD175 pKa = 4.05GNGTYY180 pKa = 10.95NSIITLYY187 pKa = 10.38SGASGATIQDD197 pKa = 3.38IEE199 pKa = 4.4ITNGNNGIYY208 pKa = 10.15GYY210 pKa = 10.61KK211 pKa = 10.04AANLTLQNLEE221 pKa = 3.72VHH223 pKa = 6.66NIGKK227 pKa = 9.72NGMIADD233 pKa = 4.67GNQTGIAMDD242 pKa = 3.86HH243 pKa = 6.88ADD245 pKa = 4.05GLKK248 pKa = 10.53LSNTSVYY255 pKa = 10.57DD256 pKa = 3.46VEE258 pKa = 4.56RR259 pKa = 11.84KK260 pKa = 10.27GIGVGDD266 pKa = 3.74TNGAQITNVNVQSVNLAANHH286 pKa = 5.83AQSHH290 pKa = 6.36DD291 pKa = 3.32AAGIKK296 pKa = 9.77FFNTNNVILKK306 pKa = 10.19DD307 pKa = 3.7SYY309 pKa = 11.16FEE311 pKa = 4.87KK312 pKa = 10.7INAFYY317 pKa = 10.43IWNDD321 pKa = 3.41TTNKK325 pKa = 7.46TTIDD329 pKa = 3.58NNNVVNVGSDD339 pKa = 3.39FLAPSFNTNVNVAGIYY355 pKa = 9.44NEE357 pKa = 4.15KK358 pKa = 10.33SSNSIVRR365 pKa = 11.84YY366 pKa = 8.59NQGTAVGDD374 pKa = 4.33FSAFNATEE382 pKa = 4.64FSTQTMTFEE391 pKa = 5.28DD392 pKa = 3.87NNSFSKK398 pKa = 10.44MEE400 pKa = 5.14FGTQDD405 pKa = 2.9YY406 pKa = 8.5WVNQEE411 pKa = 4.08AEE413 pKa = 4.16MAIATTEE420 pKa = 4.11NPDD423 pKa = 3.3EE424 pKa = 4.84ANFSLFAAEE433 pKa = 4.8YY434 pKa = 8.43YY435 pKa = 10.18AQAVIQQ441 pKa = 3.78

Molecular weight: 47.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T1CFH2|A0A2T1CFH2_9CYAN NAD(P)-dependent oxidoreductase OS=Pleurocapsa sp. CCALA 161 OX=2107688 GN=C7B62_21305 PE=4 SV=1
MM1 pKa = 6.61TQRR4 pKa = 11.84TLGGTSRR11 pKa = 11.84KK12 pKa = 7.83QKK14 pKa = 9.77RR15 pKa = 11.84KK16 pKa = 9.16SGFRR20 pKa = 11.84ARR22 pKa = 11.84KK23 pKa = 7.01RR24 pKa = 11.84TSNGRR29 pKa = 11.84RR30 pKa = 11.84VISARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8KK38 pKa = 10.1GRR40 pKa = 11.84HH41 pKa = 5.0RR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4800

0

4800

1462054

23

2812

304.6

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.042 ± 0.035

0.999 ± 0.012

5.118 ± 0.034

6.204 ± 0.032

3.897 ± 0.024

6.405 ± 0.036

1.781 ± 0.019

7.355 ± 0.032

5.246 ± 0.026

11.022 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.847 ± 0.015

4.67 ± 0.032

4.264 ± 0.026

5.523 ± 0.044

4.64 ± 0.024

6.617 ± 0.026

5.542 ± 0.028

6.279 ± 0.029

1.37 ± 0.016

3.178 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski