Candidatus Marinamargulisbacteria bacterium SCGC AG-410-N11

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Candidatus Margulisbacteria; Candidatus Marinamargulisbacteria

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328RJ29|A0A328RJ29_9BACT Phosphomethylpyrimidine synthase OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-410-N11 OX=2184345 GN=thiC PE=3 SV=1
MM1 pKa = 7.75AKK3 pKa = 10.49LILNDD8 pKa = 3.79EE9 pKa = 4.65EE10 pKa = 5.61IEE12 pKa = 4.38VPDD15 pKa = 3.99GEE17 pKa = 4.98NIVDD21 pKa = 4.13YY22 pKa = 10.71IEE24 pKa = 4.35EE25 pKa = 4.25AGVPIGCSNGVCGTCEE41 pKa = 3.9VEE43 pKa = 4.08ILGGMEE49 pKa = 4.02NLSEE53 pKa = 4.14RR54 pKa = 11.84NQEE57 pKa = 3.84EE58 pKa = 4.72DD59 pKa = 3.66DD60 pKa = 4.43LGMEE64 pKa = 4.3GDD66 pKa = 3.74MRR68 pKa = 11.84LGCQCVINKK77 pKa = 7.69GTVKK81 pKa = 8.21MTYY84 pKa = 10.24

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328RKI3|A0A328RKI3_9BACT DUF1338 domain-containing protein OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-410-N11 OX=2184345 GN=DID75_04275 PE=4 SV=1
MM1 pKa = 7.55AKK3 pKa = 10.36VSMINRR9 pKa = 11.84NKK11 pKa = 10.11KK12 pKa = 9.54KK13 pKa = 10.64KK14 pKa = 9.07NTIQYY19 pKa = 10.4RR20 pKa = 11.84NRR22 pKa = 11.84CQICGRR28 pKa = 11.84PRR30 pKa = 11.84GFLRR34 pKa = 11.84FFGMCRR40 pKa = 11.84ICVRR44 pKa = 11.84TLAGEE49 pKa = 4.44GCLPGVTKK57 pKa = 10.78SSWW60 pKa = 2.87

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1171

0

1171

466208

37

4857

398.1

45.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.641 ± 0.066

1.065 ± 0.026

5.521 ± 0.078

5.329 ± 0.077

5.048 ± 0.065

5.15 ± 0.091

2.058 ± 0.037

9.092 ± 0.079

8.359 ± 0.094

10.267 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.845 ± 0.032

6.896 ± 0.094

3.483 ± 0.044

4.293 ± 0.051

3.243 ± 0.045

8.148 ± 0.065

5.745 ± 0.068

5.379 ± 0.07

0.817 ± 0.025

3.62 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski