Blastococcus sp. CT_GayMR20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4483 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9PS02|A0A4Y9PS02_9ACTN DegT/DnrJ/EryC1/StrS family aminotransferase OS=Blastococcus sp. CT_GayMR20 OX=2559609 GN=E4P40_14420 PE=3 SV=1
MM1 pKa = 8.19DD2 pKa = 5.53GPTEE6 pKa = 4.07PNQRR10 pKa = 11.84MVPSMRR16 pKa = 11.84SARR19 pKa = 11.84LTASALATAMLMFVAACGDD38 pKa = 3.88DD39 pKa = 3.93GGGGAADD46 pKa = 4.28GGNEE50 pKa = 4.1GGSGGGDD57 pKa = 3.39APRR60 pKa = 11.84IAAVFSGTTTDD71 pKa = 3.62ADD73 pKa = 3.94YY74 pKa = 10.73TFLGLEE80 pKa = 4.15ALQAAEE86 pKa = 4.14QEE88 pKa = 4.62HH89 pKa = 6.16GAEE92 pKa = 4.2TTYY95 pKa = 10.96SEE97 pKa = 4.66SVQVPDD103 pKa = 3.4AEE105 pKa = 4.23RR106 pKa = 11.84VLRR109 pKa = 11.84EE110 pKa = 4.0YY111 pKa = 10.97VADD114 pKa = 4.89GYY116 pKa = 8.55TVIWTHH122 pKa = 6.38GSQFYY127 pKa = 10.74DD128 pKa = 3.03ATVAVAEE135 pKa = 4.21EE136 pKa = 4.29APDD139 pKa = 3.49VVFIAEE145 pKa = 4.22QDD147 pKa = 3.68TEE149 pKa = 4.24PADD152 pKa = 3.56VPDD155 pKa = 4.26NVWVLDD161 pKa = 4.16RR162 pKa = 11.84NFHH165 pKa = 6.39LAFYY169 pKa = 9.81PLGVLAAAASQTGNIAYY186 pKa = 10.17LGGVTLPFSYY196 pKa = 10.81SEE198 pKa = 3.96VHH200 pKa = 6.63AVEE203 pKa = 4.05QALADD208 pKa = 4.02SGSDD212 pKa = 3.21ATFTPVWTGDD222 pKa = 3.43FNDD225 pKa = 3.91PTKK228 pKa = 10.65AQQFASQLLADD239 pKa = 4.61GNDD242 pKa = 3.41VVLTSLNLGAVGAFEE257 pKa = 4.62AVGDD261 pKa = 4.17AGPGVLMTAKK271 pKa = 9.5YY272 pKa = 8.6TDD274 pKa = 3.71KK275 pKa = 11.47SNLSADD281 pKa = 3.74HH282 pKa = 6.43YY283 pKa = 9.19LTSVLYY289 pKa = 10.32DD290 pKa = 3.66FSVMLDD296 pKa = 4.14DD297 pKa = 4.41ILTGIEE303 pKa = 4.02DD304 pKa = 4.47GEE306 pKa = 4.56TGGYY310 pKa = 10.57LGMSYY315 pKa = 10.34EE316 pKa = 4.29QGISLQTPQNVDD328 pKa = 2.98QAVIDD333 pKa = 3.9EE334 pKa = 4.46VEE336 pKa = 4.25QIVEE340 pKa = 3.83QLAAGEE346 pKa = 4.38IEE348 pKa = 4.32VEE350 pKa = 3.91RR351 pKa = 11.84DD352 pKa = 3.41VTPVEE357 pKa = 4.13

Molecular weight: 37.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9NPH4|A0A4Y9NPH4_9ACTN Uncharacterized protein OS=Blastococcus sp. CT_GayMR20 OX=2559609 GN=E4P40_22130 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.25TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4483

0

4483

1354267

24

1960

302.1

32.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.83 ± 0.058

0.678 ± 0.01

6.353 ± 0.03

5.724 ± 0.033

2.656 ± 0.023

9.513 ± 0.037

2.019 ± 0.018

3.115 ± 0.026

1.51 ± 0.023

10.674 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.699 ± 0.013

1.542 ± 0.017

6.12 ± 0.029

2.703 ± 0.019

8.084 ± 0.046

5.023 ± 0.029

5.907 ± 0.035

9.629 ± 0.039

1.452 ± 0.014

1.769 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski