Blackfly microvirus SF02

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae; Microvirus

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8PK03|A0A4P8PK03_9VIRU Major capsid protein OS=Blackfly microvirus SF02 OX=2576452 PE=3 SV=1
MM1 pKa = 7.37EE2 pKa = 4.66VNMSKK7 pKa = 10.49VSRR10 pKa = 11.84FQGAQAEE17 pKa = 4.24NDD19 pKa = 3.59FFKK22 pKa = 11.42SNGSDD27 pKa = 2.93TAVFNRR33 pKa = 11.84EE34 pKa = 3.53PSLTRR39 pKa = 11.84QEE41 pKa = 4.17FAEE44 pKa = 4.38EE45 pKa = 3.91CDD47 pKa = 3.24INQLMKK53 pKa = 10.53RR54 pKa = 11.84YY55 pKa = 8.85DD56 pKa = 3.29GHH58 pKa = 8.35VIGGPGNLPVRR69 pKa = 11.84EE70 pKa = 3.86PMYY73 pKa = 10.86FDD75 pKa = 5.44FADD78 pKa = 4.47LPQDD82 pKa = 3.28LMGYY86 pKa = 7.97LTFMNEE92 pKa = 3.33AQAQFMSLPAAVRR105 pKa = 11.84KK106 pKa = 9.92EE107 pKa = 3.77FDD109 pKa = 3.3NDD111 pKa = 3.26PVEE114 pKa = 4.72FVAYY118 pKa = 10.16ASDD121 pKa = 3.97PSSLEE126 pKa = 3.74QMRR129 pKa = 11.84SWGLAPPAKK138 pKa = 9.97PPEE141 pKa = 4.54TPVPPPPGEE150 pKa = 4.55GPASASKK157 pKa = 10.74GSSEE161 pKa = 4.08PPTHH165 pKa = 7.0GSTT168 pKa = 3.97

Molecular weight: 18.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1F5E5|A0A4V1F5E5_9VIRU Nonstructural protein OS=Blackfly microvirus SF02 OX=2576452 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84CHH4 pKa = 5.3QAVVSVHH11 pKa = 6.02LVRR14 pKa = 11.84ALVILIRR21 pKa = 11.84RR22 pKa = 11.84IFLLVSQCVAVFGCKK37 pKa = 8.95MGCVKK42 pKa = 10.11PLKK45 pKa = 10.36AFWRR49 pKa = 11.84SRR51 pKa = 11.84SRR53 pKa = 11.84DD54 pKa = 3.5AITFDD59 pKa = 3.35INKK62 pKa = 8.99SATRR66 pKa = 11.84ISFSLPCGRR75 pKa = 11.84CIGCRR80 pKa = 11.84MEE82 pKa = 5.84KK83 pKa = 10.13ARR85 pKa = 11.84QWGLRR90 pKa = 11.84CLHH93 pKa = 6.31EE94 pKa = 4.94KK95 pKa = 10.49KK96 pKa = 10.39LWAHH100 pKa = 4.84NHH102 pKa = 4.89YY103 pKa = 9.25VTLTYY108 pKa = 10.67NDD110 pKa = 3.6EE111 pKa = 4.12FLPPGGSVCLRR122 pKa = 11.84DD123 pKa = 3.38VQLFMKK129 pKa = 10.4KK130 pKa = 9.49LRR132 pKa = 11.84KK133 pKa = 9.62AKK135 pKa = 10.21GSCKK139 pKa = 9.62EE140 pKa = 3.75NPVRR144 pKa = 11.84FFLGAEE150 pKa = 3.97YY151 pKa = 10.77GDD153 pKa = 3.98EE154 pKa = 4.22NKK156 pKa = 10.21RR157 pKa = 11.84PHH159 pKa = 5.21YY160 pKa = 10.18HH161 pKa = 7.09ALLFNCEE168 pKa = 3.74FNDD171 pKa = 4.32RR172 pKa = 11.84VFWTYY177 pKa = 10.35NKK179 pKa = 10.11RR180 pKa = 11.84GEE182 pKa = 4.1PLYY185 pKa = 10.38TSRR188 pKa = 11.84EE189 pKa = 4.14LTDD192 pKa = 3.03LWGAGHH198 pKa = 7.01CSLGEE203 pKa = 3.94VTFDD207 pKa = 3.85SAVYY211 pKa = 8.51CAKK214 pKa = 10.69YY215 pKa = 10.77AMKK218 pKa = 10.41KK219 pKa = 8.28ITISDD224 pKa = 3.69HH225 pKa = 6.31SSEE228 pKa = 4.19EE229 pKa = 3.75ARR231 pKa = 11.84SEE233 pKa = 3.9FNRR236 pKa = 11.84RR237 pKa = 11.84YY238 pKa = 9.88VVYY241 pKa = 10.36DD242 pKa = 3.19EE243 pKa = 4.41YY244 pKa = 11.9GEE246 pKa = 4.16VFEE249 pKa = 5.28RR250 pKa = 11.84SPEE253 pKa = 3.86FAVMSRR259 pKa = 11.84RR260 pKa = 11.84PGIGAGYY267 pKa = 8.92YY268 pKa = 10.39DD269 pKa = 3.89RR270 pKa = 11.84FGSEE274 pKa = 3.45IRR276 pKa = 11.84THH278 pKa = 7.02DD279 pKa = 3.6SVVVNGRR286 pKa = 11.84EE287 pKa = 3.8VRR289 pKa = 11.84PTRR292 pKa = 11.84FYY294 pKa = 11.11DD295 pKa = 3.58VRR297 pKa = 11.84SEE299 pKa = 4.03RR300 pKa = 11.84CDD302 pKa = 3.39PEE304 pKa = 4.17RR305 pKa = 11.84FAVVKK310 pKa = 10.74AEE312 pKa = 4.54RR313 pKa = 11.84KK314 pKa = 8.32RR315 pKa = 11.84QAVKK319 pKa = 10.88DD320 pKa = 3.79SVKK323 pKa = 10.89ADD325 pKa = 3.48NTPEE329 pKa = 3.89RR330 pKa = 11.84LRR332 pKa = 11.84VKK334 pKa = 10.35EE335 pKa = 4.03VLLEE339 pKa = 4.08AASKK343 pKa = 10.51KK344 pKa = 10.23KK345 pKa = 10.08EE346 pKa = 3.72RR347 pKa = 11.84SLL349 pKa = 3.86

Molecular weight: 40.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1422

90

553

284.4

31.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.861 ± 1.081

1.406 ± 0.76

4.852 ± 0.369

5.415 ± 1.601

5.696 ± 0.984

7.032 ± 0.491

1.758 ± 0.463

3.376 ± 0.436

4.36 ± 1.244

7.173 ± 0.493

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.672 ± 0.659

4.219 ± 0.448

6.118 ± 1.21

4.29 ± 0.982

7.173 ± 1.073

8.579 ± 0.977

6.399 ± 1.287

6.048 ± 0.993

1.266 ± 0.128

3.305 ± 0.772
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski