Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Kocuria; Kocuria rhizophila

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2352 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2GK89|B2GK89_KOCRD Hypothetical membrane protein OS=Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) OX=378753 GN=KRH_07620 PE=4 SV=1
MM1 pKa = 6.91NTTVNVSGMTCGHH14 pKa = 6.4CVSSVTEE21 pKa = 4.17EE22 pKa = 3.84LSEE25 pKa = 4.23IPGVTSVDD33 pKa = 3.26VDD35 pKa = 3.49LHH37 pKa = 7.42AGGTSPVSITSEE49 pKa = 4.03RR50 pKa = 11.84EE51 pKa = 3.69LSPEE55 pKa = 4.27EE56 pKa = 3.45ISAAVTEE63 pKa = 4.22AGYY66 pKa = 8.19TVEE69 pKa = 4.42

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2GJH3|B2GJH3_KOCRD Uncharacterized protein OS=Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) OX=378753 GN=KRH_00550 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.04RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84KK16 pKa = 8.24HH17 pKa = 4.59GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIGARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.77GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2352

0

2352

793120

31

1778

337.2

36.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.798 ± 0.065

0.697 ± 0.014

5.863 ± 0.04

6.033 ± 0.042

2.866 ± 0.031

9.06 ± 0.045

2.438 ± 0.023

3.452 ± 0.036

2.111 ± 0.04

9.755 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.073 ± 0.019

2.005 ± 0.027

5.768 ± 0.041

3.214 ± 0.028

7.566 ± 0.058

5.697 ± 0.035

6.118 ± 0.034

9.084 ± 0.045

1.458 ± 0.021

1.944 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski