Staphylococcus phage vB_SauM_Romulus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Silviavirus; Staphylococcus virus Remus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 164 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4I0D0|M4I0D0_9CAUD Uncharacterized protein OS=Staphylococcus phage vB_SauM_Romulus OX=1235660 GN=Romulus_140C PE=4 SV=1
MM1 pKa = 7.93IIQKK5 pKa = 9.31TYY7 pKa = 7.53TCKK10 pKa = 10.51EE11 pKa = 4.11LIEE14 pKa = 4.8KK15 pKa = 9.68YY16 pKa = 10.55FNGYY20 pKa = 9.38LKK22 pKa = 10.62EE23 pKa = 4.14GTYY26 pKa = 10.29TGTSDD31 pKa = 5.26GLDD34 pKa = 3.07TYY36 pKa = 11.88YY37 pKa = 11.64LLLEE41 pKa = 4.38NNRR44 pKa = 11.84MTIGYY49 pKa = 7.45QVPLDD54 pKa = 3.27ASFIVEE60 pKa = 4.12EE61 pKa = 5.02EE62 pKa = 3.86ITNDD66 pKa = 3.59TKK68 pKa = 11.45LEE70 pKa = 4.15NCWVLLNSHH79 pKa = 6.46AKK81 pKa = 9.64EE82 pKa = 3.74LFMDD86 pKa = 3.39NTGNVSINDD95 pKa = 4.13CIEE98 pKa = 3.91IYY100 pKa = 10.7GDD102 pKa = 3.54EE103 pKa = 4.57LLTVSILIDD112 pKa = 3.31GTFINVWRR120 pKa = 11.84NDD122 pKa = 3.33DD123 pKa = 3.51YY124 pKa = 11.71FNRR127 pKa = 11.84GGEE130 pKa = 3.98

Molecular weight: 14.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4I0D8|M4I0D8_9CAUD Uncharacterized protein OS=Staphylococcus phage vB_SauM_Romulus OX=1235660 GN=Romulus_160 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 4.7HH3 pKa = 5.46VQRR6 pKa = 11.84LFRR9 pKa = 11.84IGDD12 pKa = 3.57GSKK15 pKa = 10.8APSYY19 pKa = 9.37WEE21 pKa = 4.18KK22 pKa = 11.37KK23 pKa = 6.94NTHH26 pKa = 7.21PILIGITNSLRR37 pKa = 11.84TYY39 pKa = 10.04RR40 pKa = 11.84KK41 pKa = 9.22VVSRR45 pKa = 11.84NNN47 pKa = 3.14

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

164

0

164

33969

19

1152

207.1

23.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.986 ± 0.219

0.754 ± 0.076

6.756 ± 0.147

7.56 ± 0.267

3.73 ± 0.098

5.149 ± 0.25

1.601 ± 0.091

7.51 ± 0.229

9.903 ± 0.26

8.369 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.193 ± 0.1

7.171 ± 0.216

2.594 ± 0.171

3.491 ± 0.177

3.683 ± 0.142

6.45 ± 0.2

6.541 ± 0.215

6.376 ± 0.19

0.851 ± 0.077

5.331 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski