Betaproteobacteria bacterium MOLA814

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; unclassified Betaproteobacteria

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2683 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5AHK7|V5AHK7_9PROT Uncharacterized protein OS=Betaproteobacteria bacterium MOLA814 OX=1408164 GN=MOLA814_00670 PE=4 SV=1
MM1 pKa = 7.08STVADD6 pKa = 3.73PTDD9 pKa = 3.88FEE11 pKa = 4.61MPAALIFTDD20 pKa = 3.64SAAAKK25 pKa = 10.06VADD28 pKa = 5.43LIADD32 pKa = 3.85EE33 pKa = 5.25GNPEE37 pKa = 3.76LKK39 pKa = 10.63LRR41 pKa = 11.84VFVQGGGCSGFSYY54 pKa = 10.72GFTFDD59 pKa = 5.3EE60 pKa = 4.98IANDD64 pKa = 5.29DD65 pKa = 4.08DD66 pKa = 3.69TTMQKK71 pKa = 10.67NGVSLLVDD79 pKa = 3.71AMSYY83 pKa = 10.37QYY85 pKa = 11.49LVGAEE90 pKa = 4.01IDD92 pKa = 4.04YY93 pKa = 11.28KK94 pKa = 11.42DD95 pKa = 4.58DD96 pKa = 3.7LQGAQFIIKK105 pKa = 9.24NNPGATSTCGCGSSFSAA122 pKa = 4.79

Molecular weight: 12.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4YVR6|V4YVR6_9PROT Alanine racemase OS=Betaproteobacteria bacterium MOLA814 OX=1408164 GN=dadX PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.4TKK25 pKa = 10.36GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LGLL44 pKa = 3.7

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2683

0

2683

859643

31

1974

320.4

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.733 ± 0.06

1.071 ± 0.016

5.339 ± 0.037

4.581 ± 0.046

3.617 ± 0.035

7.856 ± 0.042

2.581 ± 0.03

4.724 ± 0.031

3.743 ± 0.04

10.449 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.951 ± 0.024

3.28 ± 0.023

4.636 ± 0.026

4.407 ± 0.035

5.402 ± 0.044

6.042 ± 0.036

5.707 ± 0.031

8.094 ± 0.041

1.527 ± 0.025

2.254 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski