Brevundimonas sp. AAP58

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Brevundimonas; unclassified Brevundimonas

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2824 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1BLD6|A0A0N1BLD6_9CAUL Methyltransferase OS=Brevundimonas sp. AAP58 OX=1523422 GN=IP78_05355 PE=4 SV=1
MM1 pKa = 7.6LRR3 pKa = 11.84TTSAALSALIITAAPALATAQNKK26 pKa = 7.67TLQQVDD32 pKa = 3.77PSARR36 pKa = 11.84ATYY39 pKa = 10.62GEE41 pKa = 3.92YY42 pKa = 10.28RR43 pKa = 11.84LYY45 pKa = 11.03AGFPDD50 pKa = 4.57DD51 pKa = 4.56PYY53 pKa = 10.76IIYY56 pKa = 8.39VTAGGDD62 pKa = 3.33VNAADD67 pKa = 4.03VGNGCVGMVAFAPSAQLTYY86 pKa = 10.68RR87 pKa = 11.84AGSYY91 pKa = 10.17PLIIRR96 pKa = 11.84TDD98 pKa = 3.49ADD100 pKa = 3.65EE101 pKa = 4.69DD102 pKa = 4.01TTLLINGPDD111 pKa = 3.99GNWYY115 pKa = 10.44CDD117 pKa = 3.9DD118 pKa = 5.4DD119 pKa = 5.37SGGNQNAEE127 pKa = 4.5LRR129 pKa = 11.84WGSPPSGVYY138 pKa = 9.94DD139 pKa = 3.23IWVGTFGGGTAPAEE153 pKa = 4.0LHH155 pKa = 6.52ISEE158 pKa = 5.3LDD160 pKa = 3.53EE161 pKa = 6.05GPGPDD166 pKa = 3.75NGYY169 pKa = 9.75PDD171 pKa = 3.73TSLPATYY178 pKa = 10.59GSITLRR184 pKa = 11.84GGFTPDD190 pKa = 3.02PHH192 pKa = 6.66RR193 pKa = 11.84VRR195 pKa = 11.84LIAGGNLDD203 pKa = 3.65ATQLGSGCVGRR214 pKa = 11.84IAGAPDD220 pKa = 3.23YY221 pKa = 10.3EE222 pKa = 4.52LTYY225 pKa = 10.81RR226 pKa = 11.84PTSYY230 pKa = 11.55DD231 pKa = 2.96LTFGVNSQADD241 pKa = 3.79TTLVINGPDD250 pKa = 3.62GRR252 pKa = 11.84WYY254 pKa = 10.84CDD256 pKa = 4.04DD257 pKa = 5.4DD258 pKa = 4.84GADD261 pKa = 3.27AALNPLIRR269 pKa = 11.84FDD271 pKa = 4.02NPQSGTYY278 pKa = 9.44DD279 pKa = 2.58IWVGTFGEE287 pKa = 4.49DD288 pKa = 2.91TAEE291 pKa = 4.1AEE293 pKa = 4.6LYY295 pKa = 10.24ISEE298 pKa = 4.8LGEE301 pKa = 4.14DD302 pKa = 3.49

Molecular weight: 31.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N1BS93|A0A0N1BS93_9CAUL Polysaccharide biosynthesis protein GumN OS=Brevundimonas sp. AAP58 OX=1523422 GN=IP78_06360 PE=4 SV=1
MM1 pKa = 7.09GRR3 pKa = 11.84ARR5 pKa = 11.84TAMRR9 pKa = 11.84RR10 pKa = 11.84PSGVRR15 pKa = 11.84RR16 pKa = 11.84APLARR21 pKa = 11.84RR22 pKa = 11.84SSRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84PRR29 pKa = 11.84SASSASIRR37 pKa = 11.84RR38 pKa = 11.84LTVEE42 pKa = 3.53TATFMRR48 pKa = 11.84RR49 pKa = 11.84ATRR52 pKa = 11.84LRR54 pKa = 11.84LRR56 pKa = 11.84SSSTSRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84SRR66 pKa = 11.84LARR69 pKa = 11.84STRR72 pKa = 11.84RR73 pKa = 11.84SCVFRR78 pKa = 11.84KK79 pKa = 9.68SALRR83 pKa = 11.84PP84 pKa = 3.51

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2824

0

2824

853354

41

1949

302.2

32.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.616 ± 0.076

0.696 ± 0.014

6.03 ± 0.036

5.864 ± 0.036

3.516 ± 0.028

9.03 ± 0.049

1.733 ± 0.022

4.539 ± 0.03

2.484 ± 0.035

9.868 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.02

2.296 ± 0.03

5.529 ± 0.034

3.062 ± 0.025

7.734 ± 0.046

5.0 ± 0.032

5.51 ± 0.033

7.709 ± 0.033

1.493 ± 0.022

2.018 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski