Klebsiella variicola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5560 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087FPU8|A0A087FPU8_KLEVA Probable malate:quinone oxidoreductase OS=Klebsiella variicola OX=244366 GN=mqo PE=3 SV=1
MM1 pKa = 7.67NIGLFYY7 pKa = 10.91GSSTCYY13 pKa = 10.49TEE15 pKa = 3.84MAAEE19 pKa = 5.12KK20 pKa = 10.0IRR22 pKa = 11.84DD23 pKa = 3.65IIGPEE28 pKa = 4.02LVTLHH33 pKa = 6.12NLKK36 pKa = 10.49DD37 pKa = 3.83DD38 pKa = 4.23SPALMSQYY46 pKa = 11.27DD47 pKa = 3.97VLILGIPTWDD57 pKa = 3.26FGEE60 pKa = 4.22IQEE63 pKa = 4.35DD64 pKa = 4.04WEE66 pKa = 4.56AVWEE70 pKa = 4.07QLDD73 pKa = 3.89TLDD76 pKa = 5.23LEE78 pKa = 4.8GKK80 pKa = 9.68IVALYY85 pKa = 11.09GMGDD89 pKa = 3.29QLGYY93 pKa = 11.02GEE95 pKa = 4.87WFLDD99 pKa = 3.74ALGMLHH105 pKa = 7.54DD106 pKa = 4.49KK107 pKa = 10.83LATKK111 pKa = 10.04GVKK114 pKa = 9.5FVGYY118 pKa = 9.0WPTEE122 pKa = 4.03GYY124 pKa = 10.61EE125 pKa = 4.19FTSPKK130 pKa = 10.02PVIADD135 pKa = 3.41GQLFVGLALDD145 pKa = 3.64EE146 pKa = 4.38TNQYY150 pKa = 10.89DD151 pKa = 4.3LSDD154 pKa = 3.61EE155 pKa = 5.41RR156 pKa = 11.84IQSWCEE162 pKa = 3.63QILGEE167 pKa = 4.17MAEE170 pKa = 4.4HH171 pKa = 7.09FSS173 pKa = 3.73

Molecular weight: 19.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B7G5H3|A0A0B7G5H3_KLEVA Metabolite and drug efflux pump OS=Klebsiella variicola OX=244366 GN=yddG PE=4 SV=1
MM1 pKa = 7.57ASVSISCPSCSATDD15 pKa = 3.7GVVRR19 pKa = 11.84NGKK22 pKa = 8.27STAGHH27 pKa = 4.88QRR29 pKa = 11.84YY30 pKa = 8.94LCSHH34 pKa = 6.46CRR36 pKa = 11.84KK37 pKa = 7.49TWQLQFTYY45 pKa = 9.73TASQPGTHH53 pKa = 5.79QKK55 pKa = 10.33IIDD58 pKa = 3.52MAMNGVGCRR67 pKa = 11.84ATARR71 pKa = 11.84IMGVGLNTILRR82 pKa = 11.84HH83 pKa = 5.55LKK85 pKa = 10.06NSGRR89 pKa = 11.84SRR91 pKa = 3.37

Molecular weight: 9.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5560

0

5560

1696576

20

4197

305.1

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.423 ± 0.041

1.077 ± 0.012

5.106 ± 0.033

5.424 ± 0.038

3.824 ± 0.026

7.566 ± 0.032

2.313 ± 0.019

5.605 ± 0.028

3.853 ± 0.033

11.093 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.016

3.431 ± 0.025

4.628 ± 0.021

4.549 ± 0.029

5.947 ± 0.033

5.821 ± 0.025

5.269 ± 0.025

7.05 ± 0.025

1.578 ± 0.017

2.72 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski