Acetitomaculum ruminis DSM 5522

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Acetitomaculum; Acetitomaculum ruminis

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2632 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0W1J0|A0A1I0W1J0_9FIRM Ribosomal 50S subunit-recycling heat shock protein contains S4 domain OS=Acetitomaculum ruminis DSM 5522 OX=1120918 GN=SAMN05216249_10327 PE=4 SV=1
NN1 pKa = 7.73DD2 pKa = 3.93KK3 pKa = 10.85LYY5 pKa = 11.28VGIVDD10 pKa = 4.21EE11 pKa = 4.89EE12 pKa = 4.3NLTEE16 pKa = 4.6DD17 pKa = 3.67GKK19 pKa = 10.94IIEE22 pKa = 4.45VDD24 pKa = 3.6MEE26 pKa = 4.63NDD28 pKa = 4.03TVLHH32 pKa = 6.17TYY34 pKa = 10.76KK35 pKa = 10.56LDD37 pKa = 3.78LLITEE42 pKa = 5.18FVVQDD47 pKa = 4.12DD48 pKa = 4.14YY49 pKa = 12.13LYY51 pKa = 10.94LYY53 pKa = 10.58DD54 pKa = 5.41DD55 pKa = 3.99NNVVYY60 pKa = 9.82KK61 pKa = 10.94YY62 pKa = 11.25KK63 pKa = 10.03MDD65 pKa = 3.93GDD67 pKa = 4.3KK68 pKa = 11.27LDD70 pKa = 5.49LICSHH75 pKa = 7.41DD76 pKa = 4.45LLNDD80 pKa = 4.37GYY82 pKa = 11.06NFVSGIFTKK91 pKa = 10.75

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1A9I5|A0A1I1A9I5_9FIRM Energy-coupling factor transport system permease protein OS=Acetitomaculum ruminis DSM 5522 OX=1120918 GN=SAMN05216249_12320 PE=4 SV=1
MM1 pKa = 8.19PYY3 pKa = 10.61NKK5 pKa = 9.53NDD7 pKa = 2.83SSMKK11 pKa = 9.65RR12 pKa = 11.84RR13 pKa = 11.84GGRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.27KK20 pKa = 9.73VCVFCGKK27 pKa = 10.59DD28 pKa = 3.18NVIDD32 pKa = 3.9YY33 pKa = 11.17KK34 pKa = 10.78DD35 pKa = 3.45TNKK38 pKa = 10.31LKK40 pKa = 10.49RR41 pKa = 11.84YY42 pKa = 8.14ISEE45 pKa = 4.0RR46 pKa = 11.84GKK48 pKa = 10.09ILPRR52 pKa = 11.84RR53 pKa = 11.84ITGNCAKK60 pKa = 9.81HH61 pKa = 5.44QRR63 pKa = 11.84ALTVAIKK70 pKa = 10.29RR71 pKa = 11.84ARR73 pKa = 11.84HH74 pKa = 4.72VALMPYY80 pKa = 9.6VAEE83 pKa = 4.23

Molecular weight: 9.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2632

0

2632

888260

26

2970

337.5

38.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.469 ± 0.052

1.352 ± 0.019

6.168 ± 0.034

7.621 ± 0.055

4.359 ± 0.038

6.407 ± 0.043

1.395 ± 0.021

8.534 ± 0.053

8.886 ± 0.048

8.57 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.028

5.68 ± 0.041

2.725 ± 0.025

2.388 ± 0.021

3.394 ± 0.037

6.445 ± 0.044

5.214 ± 0.048

6.578 ± 0.036

0.687 ± 0.013

4.377 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski