Lactococcus phage SK1 (Lactococcus lactis bacteriophage SK1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O21878|TTTP_BPLSK Probable tail terminator protein OS=Lactococcus phage SK1 OX=31532 PE=3 SV=1
MM1 pKa = 7.56EE2 pKa = 5.5FDD4 pKa = 6.13SYY6 pKa = 11.42IDD8 pKa = 3.32WYY10 pKa = 11.58NNLLTMPLNDD20 pKa = 3.86VILGVKK26 pKa = 8.96DD27 pKa = 3.57TIEE30 pKa = 4.55DD31 pKa = 3.52KK32 pKa = 10.66TVYY35 pKa = 10.62LSLSDD40 pKa = 3.67SKK42 pKa = 11.01VIKK45 pKa = 9.72MDD47 pKa = 3.07NTSFVMGYY55 pKa = 9.14YY56 pKa = 10.49

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O21891|O21891_BPLSK Uncharacterized protein OS=Lactococcus phage SK1 OX=31532 PE=4 SV=1
MM1 pKa = 7.67ILHH4 pKa = 7.18KK5 pKa = 8.45YY6 pKa = 6.14TRR8 pKa = 11.84KK9 pKa = 9.83INSSKK14 pKa = 10.47YY15 pKa = 8.74PRR17 pKa = 11.84STARR21 pKa = 11.84KK22 pKa = 8.61IANDD26 pKa = 3.76LNKK29 pKa = 10.13NDD31 pKa = 4.17PFNNYY36 pKa = 8.99LVSLEE41 pKa = 4.65LGSKK45 pKa = 10.05RR46 pKa = 11.84YY47 pKa = 9.75IIEE50 pKa = 3.88KK51 pKa = 10.63FEE53 pKa = 4.11IKK55 pKa = 10.42GMNRR59 pKa = 3.04

Molecular weight: 7.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

8449

39

999

156.5

17.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.001 ± 0.577

0.746 ± 0.203

5.622 ± 0.266

7.93 ± 0.735

4.45 ± 0.3

6.249 ± 0.753

1.148 ± 0.178

7.042 ± 0.29

9.054 ± 0.497

8.687 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.215

6.32 ± 0.346

2.083 ± 0.261

3.539 ± 0.223

3.527 ± 0.295

6.486 ± 0.473

6.439 ± 0.28

6.261 ± 0.392

1.444 ± 0.176

4.367 ± 0.424
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski