Streptomyces sp. PT12

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5724 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A365ZWD7|A0A365ZWD7_9ACTN GNAT family N-acetyltransferase OS=Streptomyces sp. PT12 OX=1510197 GN=DEH69_03005 PE=4 SV=1
PP1 pKa = 6.84GASVSTGANFTYY13 pKa = 10.34SGANAAPTAFSVNGVVCGDD32 pKa = 4.21DD33 pKa = 4.13GGPGPDD39 pKa = 4.85PDD41 pKa = 5.47PDD43 pKa = 4.97PDD45 pKa = 4.82PDD47 pKa = 4.05PPGDD51 pKa = 3.66RR52 pKa = 11.84VDD54 pKa = 4.65NPYY57 pKa = 10.82EE58 pKa = 4.09DD59 pKa = 3.46AGVYY63 pKa = 9.63VNPDD67 pKa = 2.61WSANAAAEE75 pKa = 4.21PGGDD79 pKa = 4.19AIADD83 pKa = 3.7EE84 pKa = 4.67PTAVWLDD91 pKa = 3.35RR92 pKa = 11.84RR93 pKa = 11.84AAITGGSAGNSTGLRR108 pKa = 11.84EE109 pKa = 4.31HH110 pKa = 7.37LDD112 pKa = 3.3NALIQAEE119 pKa = 4.16EE120 pKa = 3.87HH121 pKa = 7.19DD122 pKa = 3.82NFVFQVVVYY131 pKa = 8.6NLPGRR136 pKa = 11.84DD137 pKa = 3.48CAALASNGEE146 pKa = 4.12LAADD150 pKa = 4.45EE151 pKa = 3.99IDD153 pKa = 3.72VYY155 pKa = 11.07KK156 pKa = 10.94EE157 pKa = 3.77EE158 pKa = 5.06FIDD161 pKa = 5.08PIAEE165 pKa = 4.08ILGDD169 pKa = 3.59PAYY172 pKa = 10.66ADD174 pKa = 3.76LRR176 pKa = 11.84IVTIIEE182 pKa = 3.95IDD184 pKa = 3.68SLPNLITNVSPRR196 pKa = 11.84EE197 pKa = 4.02TATDD201 pKa = 3.16QCDD204 pKa = 3.17EE205 pKa = 4.03MLANGNYY212 pKa = 9.54VEE214 pKa = 4.41GVSYY218 pKa = 11.19ALDD221 pKa = 3.61TLGDD225 pKa = 3.48IPNVYY230 pKa = 10.54NYY232 pKa = 9.82IDD234 pKa = 3.8AGHH237 pKa = 6.33YY238 pKa = 10.09GWIGWDD244 pKa = 3.6DD245 pKa = 3.82NFGASAEE252 pKa = 4.44LFHH255 pKa = 6.54EE256 pKa = 4.56TATTGGASVDD266 pKa = 3.83DD267 pKa = 3.78VAGFIVNTANYY278 pKa = 8.33GPSVEE283 pKa = 4.08PHH285 pKa = 5.83YY286 pKa = 11.34SIDD289 pKa = 3.51DD290 pKa = 3.9TVGGRR295 pKa = 11.84SVRR298 pKa = 11.84EE299 pKa = 4.18SEE301 pKa = 3.85WVDD304 pKa = 2.72WNRR307 pKa = 11.84YY308 pKa = 8.3IDD310 pKa = 3.78NQSFALAFRR319 pKa = 11.84DD320 pKa = 3.67EE321 pKa = 4.52LVSQGFNDD329 pKa = 4.48EE330 pKa = 3.89IGMLIDD336 pKa = 3.57TSRR339 pKa = 11.84NGWGGPDD346 pKa = 3.52RR347 pKa = 11.84PTGPGPTTDD356 pKa = 2.68VNAYY360 pKa = 10.07VEE362 pKa = 4.39GSRR365 pKa = 11.84VDD367 pKa = 4.12GRR369 pKa = 11.84IQTGNWCNQSGAGLGEE385 pKa = 4.66RR386 pKa = 11.84PTASPVPNIDD396 pKa = 2.65AYY398 pKa = 10.94AWIKK402 pKa = 10.43PPGEE406 pKa = 4.03SDD408 pKa = 3.31GSSEE412 pKa = 5.19EE413 pKa = 4.0IPNDD417 pKa = 3.14EE418 pKa = 4.17GKK420 pKa = 11.07GFDD423 pKa = 3.72RR424 pKa = 11.84MCDD427 pKa = 3.22PTYY430 pKa = 10.84EE431 pKa = 4.29GNPRR435 pKa = 11.84NNNNMSGALPDD446 pKa = 4.3APLSGHH452 pKa = 6.02WFSAQFQEE460 pKa = 4.77LLANAYY466 pKa = 8.53PPLL469 pKa = 5.25

Molecular weight: 49.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A365YS73|A0A365YS73_9ACTN Site-specific integrase OS=Streptomyces sp. PT12 OX=1510197 GN=DEH69_28125 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVIANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.82GRR40 pKa = 11.84SRR42 pKa = 11.84LSAA45 pKa = 3.74

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5724

0

5724

1969865

22

6549

344.1

36.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.433 ± 0.061

0.77 ± 0.009

6.145 ± 0.029

6.032 ± 0.033

2.659 ± 0.017

9.772 ± 0.035

2.377 ± 0.016

3.033 ± 0.02

1.362 ± 0.021

10.462 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.597 ± 0.013

1.575 ± 0.017

6.448 ± 0.034

2.425 ± 0.018

8.754 ± 0.036

4.735 ± 0.022

5.8 ± 0.027

8.225 ± 0.027

1.535 ± 0.016

1.863 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski