Negativicoccus succinicivorans DORA_17_25

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Negativicoccus; Negativicoccus succinicivorans

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W1U8X1|W1U8X1_9FIRM Uncharacterized protein OS=Negativicoccus succinicivorans DORA_17_25 OX=1403945 GN=Q612_NSC00075G0002 PE=4 SV=1
MM1 pKa = 7.45ANIAIVYY8 pKa = 6.07WTGSGNTEE16 pKa = 3.59AMANAIDD23 pKa = 4.34EE24 pKa = 4.46GAKK27 pKa = 10.34AAGAEE32 pKa = 4.3TQLSFVTDD40 pKa = 3.28VTADD44 pKa = 4.06DD45 pKa = 4.07IAAFDD50 pKa = 4.69YY51 pKa = 10.9IALGCPAMGSEE62 pKa = 4.19QLEE65 pKa = 4.42EE66 pKa = 4.17YY67 pKa = 10.61DD68 pKa = 4.47FEE70 pKa = 4.53PFYY73 pKa = 11.48EE74 pKa = 4.23EE75 pKa = 4.98LAGHH79 pKa = 7.11LAGKK83 pKa = 10.03KK84 pKa = 8.96VVLFGSYY91 pKa = 8.9AWNDD95 pKa = 4.11GQWMDD100 pKa = 3.46DD101 pKa = 2.95WAGRR105 pKa = 11.84VAEE108 pKa = 4.34TGADD112 pKa = 3.1IVADD116 pKa = 3.8PVKK119 pKa = 10.57AYY121 pKa = 10.11AYY123 pKa = 9.44PDD125 pKa = 4.03DD126 pKa = 5.49DD127 pKa = 4.49ALEE130 pKa = 4.36ACRR133 pKa = 11.84QLGAALANAA142 pKa = 4.74

Molecular weight: 15.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W1U2C2|W1U2C2_9FIRM ABC transporter OS=Negativicoccus succinicivorans DORA_17_25 OX=1403945 GN=Q612_NSC00277G0001 PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 10.27FMKK5 pKa = 9.71LTTFKK10 pKa = 10.59VKK12 pKa = 10.38YY13 pKa = 8.49YY14 pKa = 10.56NVVEE18 pKa = 4.36RR19 pKa = 11.84WVKK22 pKa = 9.99AANTRR27 pKa = 11.84VARR30 pKa = 11.84SDD32 pKa = 3.54VFLHH36 pKa = 6.04LRR38 pKa = 11.84WSEE41 pKa = 3.8LDD43 pKa = 3.7NEE45 pKa = 4.59PGQNRR50 pKa = 11.84STICRR55 pKa = 11.84ANANRR60 pKa = 11.84RR61 pKa = 11.84VGKK64 pKa = 8.99PIRR67 pKa = 11.84QADD70 pKa = 3.66VHH72 pKa = 6.31HH73 pKa = 6.98RR74 pKa = 11.84CANAVFLWLL83 pKa = 4.11

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1679

0

1679

444484

20

2944

264.7

29.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.684 ± 0.094

0.931 ± 0.024

5.409 ± 0.052

6.731 ± 0.069

3.661 ± 0.046

7.166 ± 0.059

2.051 ± 0.029

6.635 ± 0.067

5.413 ± 0.085

9.761 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.031

3.786 ± 0.082

3.933 ± 0.047

3.842 ± 0.048

5.548 ± 0.071

4.858 ± 0.046

5.884 ± 0.054

7.342 ± 0.052

1.071 ± 0.024

3.55 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski