Nestor notabilis (Kea)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091RW30|A0A091RW30_NESNO Protein disulfide-isomerase A5 (Fragment) OS=Nestor notabilis OX=176057 GN=N333_13495 PE=4 SV=1
VV1 pKa = 6.12TPGDD5 pKa = 3.62VLEE8 pKa = 4.3PHH10 pKa = 7.37AAPEE14 pKa = 4.06LQLDD18 pKa = 4.08SLYY21 pKa = 10.77LQCKK25 pKa = 9.33DD26 pKa = 3.07SHH28 pKa = 6.48KK29 pKa = 11.18ADD31 pKa = 3.59VSDD34 pKa = 3.89VLPKK38 pKa = 10.39QEE40 pKa = 4.28MQSSQCQAAASDD52 pKa = 4.13AEE54 pKa = 4.33VLHH57 pKa = 6.12TSRR60 pKa = 11.84GFLEE64 pKa = 5.74HH65 pKa = 6.2YY66 pKa = 6.43TTPEE70 pKa = 3.98VPSAKK75 pKa = 10.26APQTDD80 pKa = 3.22ATEE83 pKa = 4.63CEE85 pKa = 4.33VGLPDD90 pKa = 4.38TYY92 pKa = 11.28LSPTADD98 pKa = 3.12SCEE101 pKa = 4.25NISLATADD109 pKa = 4.43KK110 pKa = 11.09DD111 pKa = 4.1DD112 pKa = 3.96VPHH115 pKa = 6.96SIVYY119 pKa = 10.05QNEE122 pKa = 3.61EE123 pKa = 4.41GKK125 pKa = 9.57WVTDD129 pKa = 3.51LAYY132 pKa = 9.07YY133 pKa = 10.17TSFDD137 pKa = 3.77EE138 pKa = 4.71EE139 pKa = 3.89QDD141 pKa = 3.93LNLSEE146 pKa = 4.54DD147 pKa = 3.65DD148 pKa = 3.8KK149 pKa = 11.88VNEE152 pKa = 4.1EE153 pKa = 4.86FITT156 pKa = 3.8

Molecular weight: 17.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091S7N9|A0A091S7N9_NESNO Disintegrin and metalloproteinase domain-containing protein 9 (Fragment) OS=Nestor notabilis OX=176057 GN=N333_12313 PE=4 SV=1
FF1 pKa = 7.85SPIRR5 pKa = 11.84PQFNPVQPHH14 pKa = 4.51QTPVSPSLTQFSPIRR29 pKa = 11.84PQFNPVQPHH38 pKa = 4.51QTPVSPSLTQFSPIRR53 pKa = 11.84PQFNPVQPHH62 pKa = 4.51QTPVSPSLTQFSPIRR77 pKa = 11.84PQFNPVQPHH86 pKa = 4.51QTPVSPSLTQFSPIRR101 pKa = 11.84PQFNPVQPHH110 pKa = 4.51QTPVSPSLTQFSPIRR125 pKa = 11.84PQFNPVQPHH134 pKa = 4.51QTPVSPSLTQFSPIRR149 pKa = 11.84PQFNPVRR156 pKa = 11.84PP157 pKa = 3.75

Molecular weight: 17.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9137

0

9137

4004254

32

5235

438.2

49.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.355 ± 0.019

2.258 ± 0.023

5.07 ± 0.018

7.187 ± 0.032

3.928 ± 0.019

5.821 ± 0.032

2.532 ± 0.012

5.042 ± 0.022

6.457 ± 0.03

9.714 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.01

4.162 ± 0.016

5.248 ± 0.029

4.678 ± 0.023

5.129 ± 0.02

8.235 ± 0.036

5.416 ± 0.018

6.283 ± 0.022

1.19 ± 0.008

3.016 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski