Mesorhizobium oceanicum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5014 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3SN42|A0A1L3SN42_9RHIZ ABC transporter substrate-binding protein OS=Mesorhizobium oceanicum OX=1670800 GN=BSQ44_04520 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84IANTSTALALLMTLGAGPAFAAAEE26 pKa = 4.22WTFSDD31 pKa = 4.38IDD33 pKa = 3.4QDD35 pKa = 4.53GNLEE39 pKa = 4.02LSNKK43 pKa = 8.72EE44 pKa = 3.96FEE46 pKa = 4.46QVSQGAFRR54 pKa = 11.84SWDD57 pKa = 3.49TNQDD61 pKa = 2.93QRR63 pKa = 11.84LTSDD67 pKa = 3.13EE68 pKa = 4.39LDD70 pKa = 3.46RR71 pKa = 11.84GVYY74 pKa = 10.42ASWDD78 pKa = 3.44RR79 pKa = 11.84DD80 pKa = 3.22RR81 pKa = 11.84DD82 pKa = 3.78DD83 pKa = 4.67RR84 pKa = 11.84LSEE87 pKa = 4.34SEE89 pKa = 5.42FDD91 pKa = 4.02SGWLGWFGDD100 pKa = 4.41DD101 pKa = 5.03DD102 pKa = 3.84QVAYY106 pKa = 11.02SDD108 pKa = 4.31LDD110 pKa = 4.16RR111 pKa = 11.84NGDD114 pKa = 3.39AYY116 pKa = 11.24LDD118 pKa = 3.87EE119 pKa = 6.47DD120 pKa = 4.44EE121 pKa = 4.93FTAGLQQSAALDD133 pKa = 3.57DD134 pKa = 4.23WNVGDD139 pKa = 5.97EE140 pKa = 4.36GVDD143 pKa = 3.06WQNFHH148 pKa = 5.97TALYY152 pKa = 10.22GVYY155 pKa = 9.15DD156 pKa = 3.72TDD158 pKa = 3.67KK159 pKa = 11.15NAMVNQDD166 pKa = 3.11EE167 pKa = 4.49YY168 pKa = 11.71GAYY171 pKa = 10.23SDD173 pKa = 3.95TYY175 pKa = 10.97MMGDD179 pKa = 3.47MAQAGVGAEE188 pKa = 3.82EE189 pKa = 3.97TAAVGEE195 pKa = 4.6TITPQEE201 pKa = 4.6VISMSDD207 pKa = 2.82WRR209 pKa = 11.84SEE211 pKa = 3.73DD212 pKa = 3.68LYY214 pKa = 11.24LGGMSVDD221 pKa = 3.35EE222 pKa = 4.85MMDD225 pKa = 3.32DD226 pKa = 3.7MEE228 pKa = 5.09VYY230 pKa = 10.56GPTGEE235 pKa = 4.66EE236 pKa = 3.56IGSVEE241 pKa = 3.93NVVFSNDD248 pKa = 2.74GRR250 pKa = 11.84VLSLVAEE257 pKa = 4.43VGGFWDD263 pKa = 4.07MFDD266 pKa = 3.33THH268 pKa = 7.68VNVPWDD274 pKa = 3.4QVNYY278 pKa = 10.36SGEE281 pKa = 4.11DD282 pKa = 3.37KK283 pKa = 10.74IIIPVTEE290 pKa = 4.44EE291 pKa = 3.69NVEE294 pKa = 5.14DD295 pKa = 3.93YY296 pKa = 9.94STWKK300 pKa = 9.15TGYY303 pKa = 7.89LTPQDD308 pKa = 3.45AMGVEE313 pKa = 4.61VVDD316 pKa = 5.58DD317 pKa = 4.6DD318 pKa = 6.05LEE320 pKa = 4.34TSPGIFRR327 pKa = 11.84ATDD330 pKa = 3.17LMGDD334 pKa = 3.62YY335 pKa = 11.07ARR337 pKa = 11.84VKK339 pKa = 10.21GGEE342 pKa = 3.9ANGYY346 pKa = 9.54SNYY349 pKa = 10.51GYY351 pKa = 11.31VNDD354 pKa = 5.42LIIRR358 pKa = 11.84DD359 pKa = 3.96GQLQAVVVSPDD370 pKa = 2.91AGYY373 pKa = 10.73GISGPYY379 pKa = 9.52AYY381 pKa = 9.36PYY383 pKa = 10.49YY384 pKa = 10.56SRR386 pKa = 11.84GWSAGRR392 pKa = 11.84PYY394 pKa = 11.39YY395 pKa = 10.62DD396 pKa = 3.36MPYY399 pKa = 10.94DD400 pKa = 3.73RR401 pKa = 11.84ADD403 pKa = 3.49VVDD406 pKa = 5.41NEE408 pKa = 4.16PLDD411 pKa = 3.72YY412 pKa = 11.3DD413 pKa = 3.68RR414 pKa = 11.84FGVQQ418 pKa = 3.2

Molecular weight: 46.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3SVM4|A0A1L3SVM4_9RHIZ Voltage-gated sodium channel OS=Mesorhizobium oceanicum OX=1670800 GN=BSQ44_20185 PE=4 SV=1
MM1 pKa = 7.32NRR3 pKa = 11.84TTPLVLAVALFMEE16 pKa = 4.76QMDD19 pKa = 3.98STVIATSLPAIAADD33 pKa = 3.65IEE35 pKa = 4.62TNPIALKK42 pKa = 10.55LALTAYY48 pKa = 9.23LVSVAIFIPISAWMADD64 pKa = 3.17RR65 pKa = 11.84FGAKK69 pKa = 8.91RR70 pKa = 11.84VFCTAIGVFVLGSIACAMASSLGEE94 pKa = 3.73FVLARR99 pKa = 11.84FLQGMGGSMMTPVARR114 pKa = 11.84LLLVRR119 pKa = 11.84ATPRR123 pKa = 11.84SNLVSAMAWLTVPALVGPLVGPPVGGFITTYY154 pKa = 10.8VSWHH158 pKa = 6.57WIFLINVPIGITGICAAWLLLPEE181 pKa = 4.83LEE183 pKa = 4.3RR184 pKa = 11.84GIAGRR189 pKa = 11.84IDD191 pKa = 3.5FTGFALSGMAAAGIVFGLSVVSLPALPPAVGVATLALGICSGLLYY236 pKa = 10.17FRR238 pKa = 11.84HH239 pKa = 6.07ARR241 pKa = 11.84RR242 pKa = 11.84VARR245 pKa = 11.84PILDD249 pKa = 3.34LSLFRR254 pKa = 11.84NRR256 pKa = 11.84VFRR259 pKa = 11.84AAISGGSLFRR269 pKa = 11.84IGAGATPFLLPLMFQLGFGLTPFQSGMLTFAGAFGAIGMKK309 pKa = 10.15FFASTTLRR317 pKa = 11.84KK318 pKa = 10.16GGFRR322 pKa = 11.84TVLISTAIAGGIFIGVAAAFTAQTPAWAIIAVLVAGGFLRR362 pKa = 11.84SLFFTSANALVFADD376 pKa = 4.35IGDD379 pKa = 3.88EE380 pKa = 4.08RR381 pKa = 11.84AGQATAIAAVTQQISIALGVALAGGILEE409 pKa = 4.31GVAMFSGDD417 pKa = 3.5TLGASAFPIAFVLVGLVTTLAALPFLTLPPDD448 pKa = 3.77AGSAVSGHH456 pKa = 6.21RR457 pKa = 11.84VDD459 pKa = 4.37GVNSRR464 pKa = 11.84TQVVPAKK471 pKa = 10.57

Molecular weight: 48.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5014

0

5014

1537093

41

2417

306.6

33.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.288 ± 0.043

0.805 ± 0.008

5.779 ± 0.027

6.263 ± 0.037

3.905 ± 0.025

8.731 ± 0.031

1.977 ± 0.016

5.327 ± 0.026

3.228 ± 0.028

9.676 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.017

2.506 ± 0.017

5.063 ± 0.026

2.804 ± 0.02

7.241 ± 0.034

5.497 ± 0.029

5.134 ± 0.027

7.552 ± 0.028

1.335 ± 0.014

2.242 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski