Beihai tombus-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFX9|A0A1L3KFX9_9VIRU C2H2-type domain-containing protein OS=Beihai tombus-like virus 9 OX=1922730 PE=4 SV=1
MM1 pKa = 7.46NNSQSTFSPVHH12 pKa = 5.88KK13 pKa = 10.45CPVCGKK19 pKa = 9.6VFSSKK24 pKa = 10.46RR25 pKa = 11.84GLAQHH30 pKa = 6.49GQAKK34 pKa = 10.21HH35 pKa = 5.67GWTKK39 pKa = 8.41PQRR42 pKa = 11.84RR43 pKa = 11.84NRR45 pKa = 11.84SNDD48 pKa = 2.84QPTGGKK54 pKa = 10.29GLVPRR59 pKa = 11.84GNPGNTMGNKK69 pKa = 9.37KK70 pKa = 10.19RR71 pKa = 11.84VDD73 pKa = 3.84QIDD76 pKa = 5.55LITTPNWGYY85 pKa = 9.99CEE87 pKa = 4.51KK88 pKa = 10.69VFKK91 pKa = 10.86KK92 pKa = 10.4LISVGCTLDD101 pKa = 3.46GAAHH105 pKa = 5.84VVHH108 pKa = 6.92AVDD111 pKa = 4.07PACPEE116 pKa = 4.61GIPWNGIPDD125 pKa = 3.82EE126 pKa = 4.29NTTRR130 pKa = 11.84ILSRR134 pKa = 11.84RR135 pKa = 11.84IISINTITKK144 pKa = 9.19AHH146 pKa = 5.96SAVGNWDD153 pKa = 3.8FALMTCWSPHH163 pKa = 6.28AEE165 pKa = 3.82FVWFQQKK172 pKa = 8.76TGGDD176 pKa = 3.26WSGATQGQHH185 pKa = 5.46WDD187 pKa = 3.65CKK189 pKa = 10.11LSRR192 pKa = 11.84FSRR195 pKa = 11.84LDD197 pKa = 3.23WQSVTRR203 pKa = 11.84FRR205 pKa = 11.84QAFGSTTIHH214 pKa = 6.39MDD216 pKa = 3.4ASATTTEE223 pKa = 4.1GTVYY227 pKa = 10.97SMQKK231 pKa = 9.72SPEE234 pKa = 3.93RR235 pKa = 11.84YY236 pKa = 9.54LEE238 pKa = 3.96NEE240 pKa = 4.28YY241 pKa = 11.2KK242 pKa = 10.57SGLVNKK248 pKa = 8.09TVNKK252 pKa = 9.87PIATVKK258 pKa = 10.15IEE260 pKa = 4.31HH261 pKa = 7.32DD262 pKa = 3.99SDD264 pKa = 5.79SDD266 pKa = 4.09DD267 pKa = 3.45SWISAPTTSTATKK280 pKa = 9.79SKK282 pKa = 10.31VVSPTGADD290 pKa = 2.92EE291 pKa = 4.08VTARR295 pKa = 11.84LLCEE299 pKa = 4.14YY300 pKa = 10.06CGKK303 pKa = 7.86WTQADD308 pKa = 4.07MAFMDD313 pKa = 4.13PGFYY317 pKa = 9.62TAEE320 pKa = 4.09AFKK323 pKa = 10.9GVYY326 pKa = 8.9SVCRR330 pKa = 11.84LAGPRR335 pKa = 11.84VWSVGSWQDD344 pKa = 3.01EE345 pKa = 4.36GGVDD349 pKa = 3.32RR350 pKa = 11.84WRR352 pKa = 11.84VVAPLGTHH360 pKa = 6.8HH361 pKa = 7.57DD362 pKa = 3.91FAKK365 pKa = 10.62SKK367 pKa = 11.04SPLVSNDD374 pKa = 2.04IFQPFTNGWVIFTNLDD390 pKa = 3.25PKK392 pKa = 10.7AQLVIKK398 pKa = 7.18TVRR401 pKa = 11.84GYY403 pKa = 10.77EE404 pKa = 3.81MEE406 pKa = 4.31VNSEE410 pKa = 4.25SIAALDD416 pKa = 3.53THH418 pKa = 6.88FSPDD422 pKa = 3.59SDD424 pKa = 3.56PLALHH429 pKa = 7.04FEE431 pKa = 4.43TQLLRR436 pKa = 11.84GLQSGGPASDD446 pKa = 4.09NDD448 pKa = 3.72FGDD451 pKa = 3.89ILSGALDD458 pKa = 3.61VLLDD462 pKa = 3.75GLTATGGPVGAVLGPAGKK480 pKa = 9.94LLKK483 pKa = 9.59KK484 pKa = 8.71TFWRR488 pKa = 4.11

Molecular weight: 53.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFX9|A0A1L3KFX9_9VIRU C2H2-type domain-containing protein OS=Beihai tombus-like virus 9 OX=1922730 PE=4 SV=1
MM1 pKa = 6.73VWDD4 pKa = 3.69YY5 pKa = 11.61AINQLDD11 pKa = 3.51MFYY14 pKa = 11.11YY15 pKa = 10.36NDD17 pKa = 4.0SGNQHH22 pKa = 5.11TVGVQLNNQHH32 pKa = 6.06KK33 pKa = 7.67TSSMIIHH40 pKa = 7.25NIPKK44 pKa = 10.03HH45 pKa = 4.61EE46 pKa = 4.28THH48 pKa = 6.74FSLAGIDD55 pKa = 5.71KK56 pKa = 9.84KK57 pKa = 11.22FCWFGRR63 pKa = 11.84KK64 pKa = 8.69IPLRR68 pKa = 11.84LPVRR72 pKa = 11.84LIYY75 pKa = 10.57SDD77 pKa = 4.17GSFTKK82 pKa = 10.29TKK84 pKa = 9.94FKK86 pKa = 10.74KK87 pKa = 10.45INTKK91 pKa = 10.42KK92 pKa = 10.34YY93 pKa = 10.5DD94 pKa = 4.04FIVNYY99 pKa = 10.08QGGSEE104 pKa = 4.23YY105 pKa = 11.0EE106 pKa = 4.19HH107 pKa = 6.64VLPQPEE113 pKa = 4.23PQVPIEE119 pKa = 3.74PHH121 pKa = 5.96HH122 pKa = 6.61ANKK125 pKa = 10.4EE126 pKa = 4.27PTSHH130 pKa = 7.12KK131 pKa = 10.6EE132 pKa = 3.58VDD134 pKa = 3.85EE135 pKa = 4.38TGMAHH140 pKa = 6.13MDD142 pKa = 3.24KK143 pKa = 10.45TKK145 pKa = 10.7TMLNHH150 pKa = 6.08TQPKK154 pKa = 9.64LKK156 pKa = 10.02VRR158 pKa = 11.84QRR160 pKa = 11.84ARR162 pKa = 11.84TYY164 pKa = 10.56FEE166 pKa = 3.88HH167 pKa = 6.6AQAITEE173 pKa = 4.31EE174 pKa = 3.93IRR176 pKa = 11.84LNICNVQGYY185 pKa = 8.26PCDD188 pKa = 3.17ATYY191 pKa = 11.07QLGVAKK197 pKa = 10.39GIEE200 pKa = 3.91IAKK203 pKa = 9.67KK204 pKa = 10.0YY205 pKa = 8.55NWQRR209 pKa = 11.84FTQSEE214 pKa = 4.1RR215 pKa = 11.84TEE217 pKa = 4.35IIRR220 pKa = 11.84KK221 pKa = 8.58SAAAANVPDD230 pKa = 4.2ATDD233 pKa = 3.44DD234 pKa = 3.62QIRR237 pKa = 11.84NMFKK241 pKa = 9.12ITAKK245 pKa = 9.73WIKK248 pKa = 9.41KK249 pKa = 7.95QNEE252 pKa = 3.93FTTTGNAGNLNGINGKK268 pKa = 9.03VAVSLPTKK276 pKa = 10.29KK277 pKa = 10.29AGKK280 pKa = 9.75AA281 pKa = 3.24

Molecular weight: 32.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1264

281

495

421.3

47.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.12 ± 0.025

1.741 ± 0.204

5.696 ± 0.432

4.193 ± 0.372

4.193 ± 0.126

7.516 ± 0.749

3.56 ± 0.301

5.538 ± 0.742

7.674 ± 0.839

6.883 ± 0.874

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.292

4.984 ± 0.64

4.589 ± 0.469

3.877 ± 0.634

5.38 ± 0.812

5.854 ± 0.992

7.041 ± 0.742

6.171 ± 0.453

2.453 ± 0.38

3.244 ± 0.697
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski