Clostridium neonatale

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3965 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A7MIQ9|A0A2A7MIQ9_9CLOT Uracil permease OS=Clostridium neonatale OX=137838 GN=CQ394_07740 PE=3 SV=1
MM1 pKa = 7.48NKK3 pKa = 10.03NIKK6 pKa = 10.02KK7 pKa = 10.2IIAFSLATGTFATCGPLTNSNLFTTKK33 pKa = 10.24AYY35 pKa = 10.48ASSDD39 pKa = 3.76DD40 pKa = 3.92ADD42 pKa = 3.97EE43 pKa = 5.32LSDD46 pKa = 5.02IDD48 pKa = 5.66VEE50 pKa = 4.74DD51 pKa = 5.03ADD53 pKa = 4.86GDD55 pKa = 4.35TIDD58 pKa = 4.97LYY60 pKa = 11.25EE61 pKa = 5.17DD62 pKa = 3.41KK63 pKa = 10.87DD64 pKa = 3.97YY65 pKa = 11.54DD66 pKa = 4.12DD67 pKa = 5.83EE68 pKa = 5.62YY69 pKa = 11.58DD70 pKa = 3.66EE71 pKa = 5.02PEE73 pKa = 4.23EE74 pKa = 4.8GEE76 pKa = 4.41TYY78 pKa = 10.68YY79 pKa = 11.39GEE81 pKa = 4.74SDD83 pKa = 3.56TKK85 pKa = 10.88KK86 pKa = 9.17ITIDD90 pKa = 3.07VDD92 pKa = 3.83GADD95 pKa = 3.69EE96 pKa = 4.8DD97 pKa = 4.19YY98 pKa = 11.74VRR100 pKa = 11.84IFKK103 pKa = 10.83GSTEE107 pKa = 4.18YY108 pKa = 10.83EE109 pKa = 4.16VGDD112 pKa = 4.66SISLDD117 pKa = 3.42SSGTTTLTIRR127 pKa = 11.84VYY129 pKa = 10.69EE130 pKa = 4.15DD131 pKa = 4.37EE132 pKa = 4.25YY133 pKa = 11.74DD134 pKa = 3.76EE135 pKa = 5.97DD136 pKa = 3.98DD137 pKa = 3.99TYY139 pKa = 11.89SSSEE143 pKa = 3.75YY144 pKa = 10.3NQYY147 pKa = 10.6KK148 pKa = 10.49LKK150 pKa = 10.51IEE152 pKa = 4.25YY153 pKa = 10.5NDD155 pKa = 5.04DD156 pKa = 5.32DD157 pKa = 6.14DD158 pKa = 7.41DD159 pKa = 6.12EE160 pKa = 7.7DD161 pKa = 6.81DD162 pKa = 5.59DD163 pKa = 6.24DD164 pKa = 6.14NEE166 pKa = 4.04LTEE169 pKa = 4.21IQMEE173 pKa = 4.56DD174 pKa = 3.77EE175 pKa = 5.46DD176 pKa = 6.44GDD178 pKa = 5.73DD179 pKa = 3.3IDD181 pKa = 6.18LYY183 pKa = 11.46EE184 pKa = 6.32DD185 pKa = 4.72DD186 pKa = 6.26DD187 pKa = 5.78YY188 pKa = 12.11DD189 pKa = 6.38DD190 pKa = 4.4EE191 pKa = 6.93LEE193 pKa = 4.88DD194 pKa = 4.47EE195 pKa = 4.59PEE197 pKa = 3.9EE198 pKa = 4.97DD199 pKa = 2.83EE200 pKa = 4.42TYY202 pKa = 10.74YY203 pKa = 11.31AEE205 pKa = 4.41SDD207 pKa = 3.52TRR209 pKa = 11.84KK210 pKa = 10.52VKK212 pKa = 10.36IDD214 pKa = 3.16VDD216 pKa = 3.75ADD218 pKa = 3.61DD219 pKa = 5.06DD220 pKa = 4.1YY221 pKa = 12.34VRR223 pKa = 11.84IFKK226 pKa = 10.79GSTAYY231 pKa = 10.05EE232 pKa = 3.75VDD234 pKa = 4.89DD235 pKa = 5.26EE236 pKa = 4.67IKK238 pKa = 10.65LDD240 pKa = 4.18SGNTTLKK247 pKa = 10.2IRR249 pKa = 11.84VYY251 pKa = 10.82DD252 pKa = 3.76DD253 pKa = 4.93EE254 pKa = 5.55YY255 pKa = 11.68DD256 pKa = 3.56EE257 pKa = 6.44DD258 pKa = 4.27EE259 pKa = 5.56DD260 pKa = 5.21YY261 pKa = 11.61SSSDD265 pKa = 3.24YY266 pKa = 11.1KK267 pKa = 10.55EE268 pKa = 4.06YY269 pKa = 10.48KK270 pKa = 10.48VKK272 pKa = 10.88VEE274 pKa = 4.02YY275 pKa = 11.13NGDD278 pKa = 4.23DD279 pKa = 4.4DD280 pKa = 6.65DD281 pKa = 5.97EE282 pKa = 7.28DD283 pKa = 5.06DD284 pKa = 4.91NEE286 pKa = 4.33VALSSLSLAGASISFNKK303 pKa = 8.31NTTTYY308 pKa = 11.03NLGVGNTTSSLTVQAIPVDD327 pKa = 3.62SDD329 pKa = 3.37NTVTINGTTVSSSEE343 pKa = 4.13NYY345 pKa = 9.49KK346 pKa = 10.61KK347 pKa = 10.59AVNLNVGNNTVTVKK361 pKa = 10.73VQDD364 pKa = 3.51EE365 pKa = 4.28DD366 pKa = 5.08GEE368 pKa = 4.87SKK370 pKa = 9.92TYY372 pKa = 9.24TVAVNRR378 pKa = 11.84SAQTVYY384 pKa = 7.59TTPGTTSNTTADD396 pKa = 3.47TTTATSKK403 pKa = 11.17YY404 pKa = 9.51GWQLVNNTWYY414 pKa = 8.67YY415 pKa = 10.11TYY417 pKa = 11.59SNGTKK422 pKa = 8.99ATGWQYY428 pKa = 11.23IGGEE432 pKa = 4.06WYY434 pKa = 10.88YY435 pKa = 11.14LDD437 pKa = 3.22SAGRR441 pKa = 11.84MKK443 pKa = 10.55TGWFKK448 pKa = 10.94DD449 pKa = 3.3TDD451 pKa = 3.72GRR453 pKa = 11.84WYY455 pKa = 10.58YY456 pKa = 10.29LQSSGAMAKK465 pKa = 8.26NTTIGGYY472 pKa = 10.34KK473 pKa = 10.03LGSNGAWIRR482 pKa = 3.62

Molecular weight: 54.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A7MKU1|A0A2A7MKU1_9CLOT Saccharopine dehydrogenase OS=Clostridium neonatale OX=137838 GN=CQ394_11560 PE=4 SV=1
MM1 pKa = 7.34NPLINMLLGSLLGGMGNNNMMGGNPLLNMLMGNMGGGMNNNMMNGNPLMNMFMGNQPNNQMGNMNNGMNNMNNSMNNMNNGMNNIPNNNMGMNPLLSMLMGNMNRR106 pKa = 11.84GMPNNNTNNNMMSNQNMMNNQNMMRR131 pKa = 11.84NNMMNNQNMNSRR143 pKa = 11.84RR144 pKa = 11.84GRR146 pKa = 3.45

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3965

0

3965

1262054

27

2958

318.3

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.523 ± 0.038

1.249 ± 0.015

5.822 ± 0.037

7.641 ± 0.052

4.342 ± 0.03

6.297 ± 0.036

1.318 ± 0.014

10.073 ± 0.049

8.968 ± 0.038

8.832 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.018

6.721 ± 0.041

2.681 ± 0.021

2.228 ± 0.015

3.127 ± 0.026

6.421 ± 0.038

4.9 ± 0.027

6.215 ± 0.036

0.724 ± 0.014

4.304 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski