Flavobacterium aciduliphilum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2535 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328Y7N1|A0A328Y7N1_9FLAO Uncharacterized protein OS=Flavobacterium aciduliphilum OX=1101402 GN=CLV55_1123 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 9.79FQPIVPMIWTNEE14 pKa = 3.89LQEE17 pKa = 4.43TVSFYY22 pKa = 11.23CDD24 pKa = 3.08VLGFSCGNYY33 pKa = 8.59NDD35 pKa = 3.31EE36 pKa = 4.6WGWAAINKK44 pKa = 9.03DD45 pKa = 3.77DD46 pKa = 4.92CEE48 pKa = 4.17MMIAKK53 pKa = 9.76PNMHH57 pKa = 6.78ISFDD61 pKa = 3.65KK62 pKa = 10.98PLFTGSFYY70 pKa = 10.82IKK72 pKa = 10.42VDD74 pKa = 3.7DD75 pKa = 6.35VEE77 pKa = 4.66TLWNTVKK84 pKa = 10.97DD85 pKa = 3.97KK86 pKa = 11.79VNIVYY91 pKa = 10.41DD92 pKa = 4.54LEE94 pKa = 5.48DD95 pKa = 4.45FDD97 pKa = 3.39WGMRR101 pKa = 11.84EE102 pKa = 4.04FAIYY106 pKa = 10.59DD107 pKa = 3.55NNSYY111 pKa = 9.92MIQFGQDD118 pKa = 2.76LL119 pKa = 3.79

Molecular weight: 14.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328YP46|A0A328YP46_9FLAO Multifunctional fusion protein OS=Flavobacterium aciduliphilum OX=1101402 GN=fabZ PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2535

0

2535

829962

29

4240

327.4

36.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.294 ± 0.055

0.9 ± 0.019

5.129 ± 0.04

6.266 ± 0.074

5.432 ± 0.05

6.122 ± 0.055

1.892 ± 0.026

8.064 ± 0.053

8.001 ± 0.082

9.099 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.026

6.254 ± 0.049

3.393 ± 0.033

3.572 ± 0.029

3.117 ± 0.035

6.545 ± 0.067

6.194 ± 0.108

6.239 ± 0.038

0.993 ± 0.018

4.215 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski