Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Micrandreae;

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43024 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A6MZS7|A0A6A6MZS7_HEVBR AAA domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_041282 PE=3 SV=1
MM1 pKa = 7.63GLQFDD6 pKa = 4.34VRR8 pKa = 11.84CSCVDD13 pKa = 3.45CLNVSIYY20 pKa = 10.4SQVVVLDD27 pKa = 3.63HH28 pKa = 7.06LLLKK32 pKa = 10.57GINKK36 pKa = 9.46FYY38 pKa = 10.59TRR40 pKa = 11.84WIHH43 pKa = 5.54YY44 pKa = 9.88KK45 pKa = 9.8EE46 pKa = 4.35APEE49 pKa = 4.68HH50 pKa = 5.15EE51 pKa = 4.64TSGGMLHH58 pKa = 7.22DD59 pKa = 6.03AIVDD63 pKa = 3.93MEE65 pKa = 4.3DD66 pKa = 3.62HH67 pKa = 6.51EE68 pKa = 4.98LAPFDD73 pKa = 4.42RR74 pKa = 11.84VDD76 pKa = 3.33VEE78 pKa = 4.35LNIVNVEE85 pKa = 4.21QVDD88 pKa = 3.87EE89 pKa = 4.34TLDD92 pKa = 3.33QQGRR96 pKa = 11.84DD97 pKa = 3.66FVVDD101 pKa = 3.79EE102 pKa = 4.0LHH104 pKa = 7.12EE105 pKa = 4.69RR106 pKa = 11.84DD107 pKa = 3.6EE108 pKa = 4.62TLDD111 pKa = 3.7EE112 pKa = 4.28YY113 pKa = 11.49CIDD116 pKa = 5.39EE117 pKa = 5.67DD118 pKa = 5.72DD119 pKa = 5.52LYY121 pKa = 11.67CNDD124 pKa = 5.41DD125 pKa = 4.24GEE127 pKa = 6.23DD128 pKa = 3.57DD129 pKa = 3.58

Molecular weight: 14.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A6NIL0|A0A6A6NIL0_HEVBR GRAS domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_022662 PE=3 SV=1
MM1 pKa = 7.55TSQLLRR7 pKa = 11.84KK8 pKa = 8.32WGSWDD13 pKa = 3.53LLRR16 pKa = 11.84AKK18 pKa = 10.49LGALPTRR25 pKa = 11.84ISQKK29 pKa = 9.98LGTRR33 pKa = 11.84HH34 pKa = 5.08VPVVRR39 pKa = 11.84GDD41 pKa = 4.1SGWDD45 pKa = 3.33AAGLSADD52 pKa = 3.86PEE54 pKa = 4.2TFARR58 pKa = 11.84IRR60 pKa = 11.84EE61 pKa = 4.29LEE63 pKa = 4.3VIHH66 pKa = 6.46CRR68 pKa = 11.84WAMLGALGCLFPEE81 pKa = 4.33HH82 pKa = 7.01LARR85 pKa = 11.84TEE87 pKa = 3.83LSLAKK92 pKa = 10.06QCFSRR97 pKa = 11.84LVLKK101 pKa = 10.15FSATLILTTWGFPASSMGYY120 pKa = 9.05QVVLMYY126 pKa = 10.48RR127 pKa = 11.84IAGEE131 pKa = 3.83PLVRR135 pKa = 11.84SLTRR139 pKa = 11.84YY140 pKa = 8.61LVPRR144 pKa = 11.84RR145 pKa = 11.84NLL147 pKa = 3.21

Molecular weight: 16.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43024

0

43024

16386060

49

3639

380.9

42.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.757 ± 0.011

1.867 ± 0.005

5.327 ± 0.008

6.592 ± 0.012

4.174 ± 0.007

6.624 ± 0.011

2.337 ± 0.005

5.479 ± 0.008

6.13 ± 0.011

9.854 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.005

4.586 ± 0.008

4.719 ± 0.01

3.713 ± 0.008

5.185 ± 0.009

8.778 ± 0.014

4.749 ± 0.006

6.503 ± 0.008

1.331 ± 0.004

2.797 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski