Gemmataceae bacterium

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Gemmatales; Gemmataceae; unclassified Gemmataceae

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6468 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4V0I0X3|A0A4V0I0X3_9BACT Uncharacterized protein OS=Gemmataceae bacterium OX=2052164 GN=GCJUQL4_07910 PE=4 SV=1
MM1 pKa = 7.52PRR3 pKa = 11.84TRR5 pKa = 11.84RR6 pKa = 11.84DD7 pKa = 3.4EE8 pKa = 4.36PDD10 pKa = 3.27EE11 pKa = 5.93DD12 pKa = 4.42EE13 pKa = 5.15EE14 pKa = 5.09DD15 pKa = 3.46WDD17 pKa = 6.03DD18 pKa = 4.62GDD20 pKa = 6.08DD21 pKa = 4.26YY22 pKa = 11.87DD23 pKa = 5.79PEE25 pKa = 5.52DD26 pKa = 4.73PEE28 pKa = 4.51TYY30 pKa = 9.9PAGLYY35 pKa = 10.68DD36 pKa = 5.08DD37 pKa = 6.08DD38 pKa = 5.98GPATVPCPHH47 pKa = 6.71CRR49 pKa = 11.84AEE51 pKa = 3.88ILEE54 pKa = 4.31DD55 pKa = 3.67SEE57 pKa = 4.36RR58 pKa = 11.84CPRR61 pKa = 11.84CGEE64 pKa = 4.02YY65 pKa = 10.66LSAEE69 pKa = 4.06DD70 pKa = 5.41APRR73 pKa = 11.84QLSGTAWVVLVLMLVAILLTVLGIGG98 pKa = 3.81

Molecular weight:
10.92 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4V6M2D1|A0A4V6M2D1_9BACT Uncharacterized protein OS=Gemmataceae bacterium OX=2052164 GN=GCJUQL4_57730 PE=4 SV=1
MM1 pKa = 7.36NPHH4 pKa = 7.03RR5 pKa = 11.84LPWTALAARR14 pKa = 11.84FRR16 pKa = 11.84AAAPRR21 pKa = 11.84SAAPRR26 pKa = 11.84AARR29 pKa = 11.84APRR32 pKa = 11.84AAGGHH37 pKa = 6.16PGRR40 pKa = 11.84RR41 pKa = 11.84PLNQLFAPAAAEE53 pKa = 4.15PPAAPATSATGPTASTVAA71 pKa = 3.68

Molecular weight:
7.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6468

0

6468

2271994

29

3244

351.3

37.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.887 ± 0.047

1.046 ± 0.012

5.86 ± 0.02

5.465 ± 0.025

3.541 ± 0.018

8.744 ± 0.032

2.052 ± 0.015

3.112 ± 0.02

4.089 ± 0.042

9.778 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.698 ± 0.014

2.473 ± 0.019

6.7 ± 0.037

2.733 ± 0.017

7.32 ± 0.034

4.443 ± 0.022

5.725 ± 0.035

8.583 ± 0.028

1.512 ± 0.012

2.235 ± 0.014

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski