Aeromonas phage PVN02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Chaseviridae; Nefertitivirinae; Pahsextavirus; unclassified Pahsextavirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L1C7Q2|A0A6L1C7Q2_9CAUD Peptidase m15a OS=Aeromonas phage PVN02 OX=2742446 GN=PVN02_00053 PE=4 SV=1
MM1 pKa = 7.56SNLNITAVKK10 pKa = 10.0PEE12 pKa = 4.24KK13 pKa = 10.76LCVQAVDD20 pKa = 4.95LRR22 pKa = 11.84LGDD25 pKa = 3.64NVRR28 pKa = 11.84AIVLDD33 pKa = 3.36QLQNIGGDD41 pKa = 3.3QVVAVVAGGAARR53 pKa = 11.84DD54 pKa = 3.58YY55 pKa = 10.02WAATMYY61 pKa = 11.18GLDD64 pKa = 3.85LQGSGDD70 pKa = 3.34IDD72 pKa = 3.48ICVLGFDD79 pKa = 3.86VFDD82 pKa = 4.81FEE84 pKa = 6.78SFDD87 pKa = 4.13ADD89 pKa = 3.83CLLVDD94 pKa = 3.79VANNLRR100 pKa = 11.84AHH102 pKa = 7.0GFNVDD107 pKa = 3.34KK108 pKa = 10.98AQHH111 pKa = 5.59HH112 pKa = 5.73WNIEE116 pKa = 3.98YY117 pKa = 10.26EE118 pKa = 4.23HH119 pKa = 5.87QQDD122 pKa = 3.25RR123 pKa = 11.84LALVMQMEE131 pKa = 4.72VNGLDD136 pKa = 3.33IDD138 pKa = 4.19FLFYY142 pKa = 10.89APEE145 pKa = 4.23IATLPEE151 pKa = 3.76MFEE154 pKa = 4.55TFNSPLNKK162 pKa = 9.56FALSSVVHH170 pKa = 7.03DD171 pKa = 4.09EE172 pKa = 5.19LIVEE176 pKa = 4.44NFGFCPKK183 pKa = 10.42DD184 pKa = 3.08PNYY187 pKa = 10.28IYY189 pKa = 10.23NAPVGTDD196 pKa = 2.99ALMEE200 pKa = 4.56RR201 pKa = 11.84ADD203 pKa = 4.1KK204 pKa = 10.31MYY206 pKa = 10.65QRR208 pKa = 11.84WQAVRR213 pKa = 11.84AAHH216 pKa = 7.16IGRR219 pKa = 11.84AAHH222 pKa = 6.91PYY224 pKa = 9.76ANIVEE229 pKa = 4.52PVV231 pKa = 2.9

Molecular weight: 25.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L1C705|A0A6L1C705_9CAUD DNA ligase OS=Aeromonas phage PVN02 OX=2742446 GN=PVN02_00020 PE=4 SV=1
MM1 pKa = 7.25YY2 pKa = 9.55PRR4 pKa = 11.84EE5 pKa = 4.4RR6 pKa = 11.84KK7 pKa = 7.92PQVYY11 pKa = 8.96TISYY15 pKa = 9.04RR16 pKa = 11.84LGGVWCSSQVRR27 pKa = 11.84AGSRR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 10.17AEE35 pKa = 4.03EE36 pKa = 3.67LLKK39 pKa = 11.02RR40 pKa = 11.84MLPNAEE46 pKa = 4.01QVGLSPKK53 pKa = 10.07RR54 pKa = 11.84ARR56 pKa = 11.84EE57 pKa = 3.57VDD59 pKa = 3.11

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

15473

46

1229

241.8

26.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.216 ± 0.338

1.293 ± 0.178

6.004 ± 0.251

6.799 ± 0.225

3.419 ± 0.177

7.426 ± 0.392

2.204 ± 0.107

5.468 ± 0.242

5.901 ± 0.236

7.762 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.947 ± 0.14

4.272 ± 0.167

4.466 ± 0.183

3.916 ± 0.144

5.674 ± 0.193

5.519 ± 0.225

5.726 ± 0.244

7.245 ± 0.31

1.486 ± 0.132

3.257 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski