Saccharomonospora viridis (strain ATCC 15386 / DSM 43017 / JCM 3036 / NBRC 12207 / P101)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharomonospora; Saccharomonospora viridis

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7MWE0|C7MWE0_SACVD Uncharacterized protein OS=Saccharomonospora viridis (strain ATCC 15386 / DSM 43017 / JCM 3036 / NBRC 12207 / P101) OX=471857 GN=Svir_07300 PE=4 SV=1
MM1 pKa = 7.64IGLCAALFSLWLAPAGASPGTGDD24 pKa = 4.26AVSTSAQRR32 pKa = 11.84PAQQQVLTWTADD44 pKa = 3.27DD45 pKa = 5.36RR46 pKa = 11.84MDD48 pKa = 4.1EE49 pKa = 4.34YY50 pKa = 11.74ASAPATAKK58 pKa = 10.13PGPATIVFEE67 pKa = 4.22NSEE70 pKa = 4.26NTGNTSGMSHH80 pKa = 6.96TLTFDD85 pKa = 3.31TSSPEE90 pKa = 3.99YY91 pKa = 11.17NNDD94 pKa = 2.92VQLNIVASPFDD105 pKa = 4.19ANGGRR110 pKa = 11.84YY111 pKa = 7.68EE112 pKa = 4.23VEE114 pKa = 4.19VTLSPGKK121 pKa = 10.13YY122 pKa = 8.78RR123 pKa = 11.84YY124 pKa = 8.66YY125 pKa = 10.61CAIPGHH131 pKa = 6.45GEE133 pKa = 3.8MQGVLVVTEE142 pKa = 4.45DD143 pKa = 3.38GGGEE147 pKa = 4.32EE148 pKa = 4.9DD149 pKa = 3.58TTPPEE154 pKa = 3.8VTAEE158 pKa = 4.06VTGDD162 pKa = 3.15QDD164 pKa = 4.45EE165 pKa = 4.4EE166 pKa = 4.55GNYY169 pKa = 9.69IGSATVKK176 pKa = 10.35ISAQDD181 pKa = 3.22SGSGVDD187 pKa = 3.44TVEE190 pKa = 4.01YY191 pKa = 10.83DD192 pKa = 3.17LDD194 pKa = 3.8EE195 pKa = 6.51AGFEE199 pKa = 4.72PYY201 pKa = 9.73TEE203 pKa = 4.37PLTINEE209 pKa = 4.67PGDD212 pKa = 3.56HH213 pKa = 5.49TVHH216 pKa = 6.59YY217 pKa = 9.78RR218 pKa = 11.84ATDD221 pKa = 3.41NAGNTSEE228 pKa = 4.24TGSVTFTVVEE238 pKa = 4.45GDD240 pKa = 4.03TEE242 pKa = 4.41DD243 pKa = 3.51TTPPEE248 pKa = 3.88VTAEE252 pKa = 4.06VTGDD256 pKa = 3.15QDD258 pKa = 4.45EE259 pKa = 4.4EE260 pKa = 4.55GNYY263 pKa = 9.69IGSATVKK270 pKa = 10.35ISAQDD275 pKa = 3.22SGSGVDD281 pKa = 3.44TVEE284 pKa = 4.01YY285 pKa = 10.83DD286 pKa = 3.17LDD288 pKa = 3.8EE289 pKa = 6.51AGFEE293 pKa = 4.72PYY295 pKa = 9.73TEE297 pKa = 4.37PLTINEE303 pKa = 4.67PGDD306 pKa = 3.56HH307 pKa = 5.49TVHH310 pKa = 6.59YY311 pKa = 9.78RR312 pKa = 11.84ATDD315 pKa = 3.41NAGNTSEE322 pKa = 4.24TGSVTFTVVEE332 pKa = 4.45GDD334 pKa = 4.03TEE336 pKa = 4.41DD337 pKa = 3.51TTPPEE342 pKa = 3.98VTVQLTGSQDD352 pKa = 3.53AQWNYY357 pKa = 10.25VDD359 pKa = 3.94SATVALSAHH368 pKa = 6.68DD369 pKa = 4.53PDD371 pKa = 4.94SGVHH375 pKa = 5.49FLRR378 pKa = 11.84YY379 pKa = 9.83SLDD382 pKa = 3.59GGSYY386 pKa = 8.24TAYY389 pKa = 10.53GEE391 pKa = 4.32PIVVNEE397 pKa = 4.28PGEE400 pKa = 4.34HH401 pKa = 4.72TVLYY405 pKa = 10.18HH406 pKa = 7.21AVDD409 pKa = 3.34HH410 pKa = 6.59AGNRR414 pKa = 11.84SEE416 pKa = 4.35DD417 pKa = 3.49GKK419 pKa = 9.78VTFTVVVAEE428 pKa = 4.67GDD430 pKa = 3.38ACPASDD436 pKa = 4.55IRR438 pKa = 11.84DD439 pKa = 3.58TLVIKK444 pKa = 10.71GHH446 pKa = 7.22DD447 pKa = 3.55STVANVDD454 pKa = 3.44TGNGCTLNDD463 pKa = 5.35LIDD466 pKa = 3.69EE467 pKa = 4.39HH468 pKa = 9.11AEE470 pKa = 4.05YY471 pKa = 9.98PNQGAFLAHH480 pKa = 4.95VTKK483 pKa = 9.95VTNDD487 pKa = 3.34LVADD491 pKa = 4.43GVISDD496 pKa = 3.87SEE498 pKa = 3.82QRR500 pKa = 11.84RR501 pKa = 11.84ILRR504 pKa = 11.84AAEE507 pKa = 3.7NSGVGEE513 pKa = 4.1

Molecular weight: 54.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7MSR6|C7MSR6_SACVD Uncharacterized protein OS=Saccharomonospora viridis (strain ATCC 15386 / DSM 43017 / JCM 3036 / NBRC 12207 / P101) OX=471857 GN=Svir_01940 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 5.69GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.24GRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84LSVV45 pKa = 2.85

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3828

0

3828

1244168

37

7310

325.0

35.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.125 ± 0.05

0.766 ± 0.011

6.226 ± 0.032

6.431 ± 0.037

2.851 ± 0.027

8.841 ± 0.04

2.242 ± 0.018

3.541 ± 0.03

2.168 ± 0.029

10.318 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.747 ± 0.018

1.956 ± 0.02

5.785 ± 0.032

2.698 ± 0.022

8.06 ± 0.05

5.338 ± 0.027

6.124 ± 0.027

9.259 ± 0.039

1.449 ± 0.015

2.076 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski