Emergencia timonensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XIII. Incertae Sedis; Emergencia

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3841 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A415E5X4|A0A415E5X4_9FIRM Uncharacterized protein OS=Emergencia timonensis OX=1776384 GN=DW099_00260 PE=4 SV=1
MM1 pKa = 7.53AKK3 pKa = 10.01VINMPKK9 pKa = 9.26MGEE12 pKa = 3.94AMEE15 pKa = 4.63EE16 pKa = 4.23GQIVSWMYY24 pKa = 11.07EE25 pKa = 3.62EE26 pKa = 4.79GQTVEE31 pKa = 3.8KK32 pKa = 10.84GEE34 pKa = 4.08PLFEE38 pKa = 5.5IMTDD42 pKa = 3.01KK43 pKa = 10.77STMEE47 pKa = 4.2FPCPEE52 pKa = 4.4SGTLLKK58 pKa = 10.45ILVEE62 pKa = 4.23CNEE65 pKa = 5.15DD66 pKa = 3.81YY67 pKa = 11.39DD68 pKa = 4.85CGTPICIIGAAGEE81 pKa = 4.89DD82 pKa = 3.47ISDD85 pKa = 5.08LII87 pKa = 5.05

Molecular weight: 9.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A415E175|A0A415E175_9FIRM Uncharacterized protein OS=Emergencia timonensis OX=1776384 GN=DW099_11915 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.39KK3 pKa = 10.17PEE5 pKa = 4.34SPCARR10 pKa = 11.84CTEE13 pKa = 4.11RR14 pKa = 11.84TLRR17 pKa = 11.84CHH19 pKa = 6.36GRR21 pKa = 11.84CEE23 pKa = 4.05RR24 pKa = 11.84YY25 pKa = 8.44MAFQQQNRR33 pKa = 11.84AYY35 pKa = 9.98NRR37 pKa = 11.84LVAANTGQEE46 pKa = 3.85NRR48 pKa = 11.84VKK50 pKa = 10.67YY51 pKa = 10.63SKK53 pKa = 10.77SRR55 pKa = 11.84LNRR58 pKa = 11.84MYY60 pKa = 10.85KK61 pKa = 10.21

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3841

0

3841

1242610

17

2973

323.5

36.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.004 ± 0.039

1.601 ± 0.016

5.635 ± 0.033

7.21 ± 0.048

4.246 ± 0.027

7.401 ± 0.034

1.643 ± 0.018

7.781 ± 0.037

6.778 ± 0.036

9.14 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.206 ± 0.022

4.058 ± 0.027

3.302 ± 0.019

3.014 ± 0.021

4.182 ± 0.03

5.728 ± 0.031

5.42 ± 0.033

6.877 ± 0.03

0.864 ± 0.012

3.912 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski