Microthlaspi erraticum

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Coluteocarpeae; Microthlaspi

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45482 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6D2JP36|A0A6D2JP36_9BRAS Uncharacterized protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS29973 PE=4 SV=1
MM1 pKa = 7.46EE2 pKa = 5.65PVLQVLQQSHH12 pKa = 5.81VLPPRR17 pKa = 11.84DD18 pKa = 4.21DD19 pKa = 3.87VFFDD23 pKa = 4.12DD24 pKa = 6.35DD25 pKa = 4.17EE26 pKa = 6.41VDD28 pKa = 3.66VLLKK32 pKa = 10.13EE33 pKa = 3.8IHH35 pKa = 6.34GALIFDD41 pKa = 4.77VYY43 pKa = 11.55DD44 pKa = 3.58DD45 pKa = 4.16TNPISGACVYY55 pKa = 11.05DD56 pKa = 3.45EE57 pKa = 5.15TIPILGVGDD66 pKa = 3.98GTYY69 pKa = 10.53LICDD73 pKa = 3.65EE74 pKa = 5.05GEE76 pKa = 3.97NANPVDD82 pKa = 4.47IISDD86 pKa = 4.1EE87 pKa = 3.75ISANVFIGVHH97 pKa = 4.48EE98 pKa = 4.17QEE100 pKa = 4.12IEE102 pKa = 4.2LIGVHH107 pKa = 6.04EE108 pKa = 4.53LLEE111 pKa = 4.37SRR113 pKa = 11.84NTNLFSRR120 pKa = 11.84SDD122 pKa = 3.46EE123 pKa = 4.11DD124 pKa = 4.1LSNNVCVTVVFDD136 pKa = 5.64SYY138 pKa = 11.93DD139 pKa = 3.8DD140 pKa = 5.04DD141 pKa = 5.66PIFDD145 pKa = 4.53FLLEE149 pKa = 4.19KK150 pKa = 10.82YY151 pKa = 9.67VLRR154 pKa = 11.84SLLTMRR160 pKa = 11.84VQSSIPGITKK170 pKa = 9.8IEE172 pKa = 3.98AMTYY176 pKa = 6.48TTQPTT181 pKa = 3.38

Molecular weight: 20.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6D2JYA7|A0A6D2JYA7_9BRAS Uncharacterized protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS32149 PE=4 SV=1
MM1 pKa = 7.42SWRR4 pKa = 11.84FAWRR8 pKa = 11.84LWVVSAVLWCLGSSGVSRR26 pKa = 11.84AWGAPVHH33 pKa = 6.62GRR35 pKa = 11.84FQRR38 pKa = 11.84MGSSQGAPMRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84LGQGSGAWGSGRR62 pKa = 11.84AQVRR66 pKa = 11.84GRR68 pKa = 11.84HH69 pKa = 5.09RR70 pKa = 11.84LGSGFTTIII79 pKa = 3.69

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45482

0

45482

16402413

10

5395

360.6

40.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.423 ± 0.009

1.832 ± 0.006

5.38 ± 0.008

6.821 ± 0.015

4.209 ± 0.007

6.331 ± 0.012

2.368 ± 0.005

5.044 ± 0.008

6.298 ± 0.012

9.42 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.455 ± 0.005

4.14 ± 0.008

5.11 ± 0.014

3.55 ± 0.009

5.893 ± 0.01

8.915 ± 0.013

5.138 ± 0.007

6.55 ± 0.009

1.367 ± 0.004

2.756 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski