Pseudomonas phage phiKMV

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; Phikmvvirus; Pseudomonas virus phiKMV

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7Y2C8|Q7Y2C8_BPKMV Internal core protein OS=Pseudomonas phage phiKMV OX=204270 GN=gp37 PE=4 SV=1
MM1 pKa = 7.52ARR3 pKa = 11.84FKK5 pKa = 11.05NPEE8 pKa = 4.31TIHH11 pKa = 6.06VADD14 pKa = 3.82GVEE17 pKa = 4.42AVFSLDD23 pKa = 3.36FPFLRR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 4.09DD31 pKa = 3.59VFVQVDD37 pKa = 4.16KK38 pKa = 11.22ILTTDD43 pKa = 3.65YY44 pKa = 10.74TWVDD48 pKa = 3.16NTNIQLAVVPKK59 pKa = 9.93KK60 pKa = 10.33DD61 pKa = 3.18QEE63 pKa = 4.01VRR65 pKa = 11.84IFRR68 pKa = 11.84DD69 pKa = 3.52TPAQVPDD76 pKa = 3.7TQFSQGIPFLPRR88 pKa = 11.84YY89 pKa = 9.44IDD91 pKa = 3.78ANNKK95 pKa = 7.73QLLYY99 pKa = 10.69AVQEE103 pKa = 4.83GINTANLALDD113 pKa = 4.02GVLDD117 pKa = 5.11AIRR120 pKa = 11.84IAEE123 pKa = 4.12EE124 pKa = 3.63ARR126 pKa = 11.84RR127 pKa = 11.84LAQEE131 pKa = 4.42ALDD134 pKa = 4.19AANEE138 pKa = 4.02ALRR141 pKa = 11.84RR142 pKa = 11.84ALGFAEE148 pKa = 3.77IRR150 pKa = 11.84TVTEE154 pKa = 4.95DD155 pKa = 3.95SDD157 pKa = 4.62IDD159 pKa = 4.09PSWRR163 pKa = 11.84GYY165 pKa = 7.33WNRR168 pKa = 11.84CITSEE173 pKa = 4.27QSLTLTMQMEE183 pKa = 4.91DD184 pKa = 3.93PDD186 pKa = 4.2EE187 pKa = 4.22PWIEE191 pKa = 3.83FSEE194 pKa = 4.42VHH196 pKa = 6.29FEE198 pKa = 3.83QAGIRR203 pKa = 11.84DD204 pKa = 4.21LNIVAGPGVTINRR217 pKa = 11.84LQNTTMQLYY226 pKa = 10.33GEE228 pKa = 4.7NGVCTLKK235 pKa = 10.97RR236 pKa = 11.84LGPNHH241 pKa = 7.1WIIFGAMEE249 pKa = 5.38DD250 pKa = 3.93DD251 pKa = 3.73

Molecular weight: 28.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q7Y2C0|ENLYS_BPKMV SAR-endolysin OS=Pseudomonas phage phiKMV OX=204270 GN=45 PE=1 SV=1
MM1 pKa = 7.72PRR3 pKa = 11.84TIVAILVLAVVALGASYY20 pKa = 11.15GFVQSYY26 pKa = 9.26RR27 pKa = 11.84ALGIAQGEE35 pKa = 4.76IKK37 pKa = 10.4RR38 pKa = 11.84QTARR42 pKa = 11.84AEE44 pKa = 4.03ALEE47 pKa = 4.04VRR49 pKa = 11.84YY50 pKa = 8.34ATLQRR55 pKa = 11.84HH56 pKa = 5.04VKK58 pKa = 9.62EE59 pKa = 4.09VAARR63 pKa = 11.84TNTQRR68 pKa = 11.84QEE70 pKa = 3.19VDD72 pKa = 2.73RR73 pKa = 11.84ALDD76 pKa = 3.59QNRR79 pKa = 11.84PWADD83 pKa = 3.33RR84 pKa = 11.84PVPAAVVDD92 pKa = 4.23SLCNRR97 pKa = 11.84PGARR101 pKa = 11.84CAVRR105 pKa = 11.84TPTDD109 pKa = 2.88

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12973

64

1337

264.8

29.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.192 ± 0.521

1.002 ± 0.171

5.874 ± 0.195

6.097 ± 0.242

3.191 ± 0.162

7.909 ± 0.306

2.104 ± 0.179

4.27 ± 0.228

4.679 ± 0.289

9.057 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.991 ± 0.143

3.577 ± 0.224

4.61 ± 0.384

4.679 ± 0.287

6.837 ± 0.315

4.887 ± 0.217

5.627 ± 0.283

6.83 ± 0.264

1.588 ± 0.12

2.999 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski