Proteome-pI 2.0 - Proteome Isoelectric Point Database

Streptomyces cattleya (strain ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces cattleya

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 7537 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|F8K2P5|F8K2P5_STREN Putative glycine betaine ABC transporter substrate-binding and permease component OS=Streptomyces cattleya (strain ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057) OX=1003195 GN=SCATT_51840 PE=3 SV=1

MM1 pKa = 7.67SDD3 pKa = 2.94ATGEE7 pKa = 4.04EE8 pKa = 4.39LEE10 pKa = 4.27VWIDD14 pKa = 3.43QDD16 pKa = 3.99LCTGDD21 pKa = 5.01GICAQYY27 pKa = 10.9APEE30 pKa = 4.23VFEE33 pKa = 5.9LDD35 pKa = 3.05IDD37 pKa = 3.65GLAYY41 pKa = 10.47VKK43 pKa = 10.82APAEE47 pKa = 4.2PGEE50 pKa = 4.19EE51 pKa = 4.44GEE53 pKa = 5.26LRR55 pKa = 11.84TEE57 pKa = 4.57PGATAPVPLPLLHH70 pKa = 7.33DD71 pKa = 4.03VVDD74 pKa = 4.24SAKK77 pKa = 9.86EE78 pKa = 3.93CPGEE82 pKa = 4.38CIHH85 pKa = 6.4VRR87 pKa = 11.84RR88 pKa = 11.84VSDD91 pKa = 3.27KK92 pKa = 10.85VEE94 pKa = 4.17VYY96 pKa = 10.9GPDD99 pKa = 3.2AAA101 pKa = 5.72

Molecular weight: 10.78 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|F8JSK5|F8JSK5_STREN Histidine kinase OS=Streptomyces cattleya (strain ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057) OX=1003195 GN=SCATT_43600 PE=4 SV=1

MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
7537	0	7537	2358060	37	14231	312.9	33.39

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
14.437 ± 0.047	0.853 ± 0.009	5.851 ± 0.027	5.306 ± 0.033	2.596 ± 0.015	9.668 ± 0.031	2.535 ± 0.014	2.772 ± 0.02	1.694 ± 0.024	10.171 ± 0.04

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.638 ± 0.013	1.558 ± 0.015	6.717 ± 0.031	2.549 ± 0.018	8.818 ± 0.038	4.512 ± 0.02	6.202 ± 0.031	8.619 ± 0.031	1.503 ± 0.011	1.999 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski