Ochrobactrum intermedium 229E

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Brucellaceae; Brucella/Ochrobactrum group; Brucella; Brucella intermedia

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U4V7R8|U4V7R8_9RHIZ Dihydroxy-acid dehydratase OS=Ochrobactrum intermedium 229E OX=1337887 GN=Q644_25235 PE=3 SV=1
SS1 pKa = 7.36DD2 pKa = 3.89AATTTVNVLVDD13 pKa = 4.24GVPYY17 pKa = 10.53AAVSNGDD24 pKa = 3.82GTWTASVPGSALAGAATPTVTAEE47 pKa = 4.19VVFADD52 pKa = 4.68AAGNDD57 pKa = 3.89AAPVTSTQTYY67 pKa = 7.88TVDD70 pKa = 3.28VTPPTTDD77 pKa = 2.97VAITDD82 pKa = 4.04PEE84 pKa = 4.95GDD86 pKa = 4.16NIPTASGTTEE96 pKa = 4.06PGSTVVVTWPDD107 pKa = 3.14GTTSQVTADD116 pKa = 3.74GTTGAWTATAVLAQPAGTVSVAVTDD141 pKa = 3.94PAGNPGSGSGGWTPSTTTPGAADD164 pKa = 3.95DD165 pKa = 4.3LAAASVDD172 pKa = 3.76VVPTEE177 pKa = 4.07TAVDD181 pKa = 3.73NGDD184 pKa = 3.1ATYY187 pKa = 11.41LLGIGIGLGIIDD199 pKa = 4.68LQATVLGVPSVGFTITEE216 pKa = 4.08GHH218 pKa = 5.52TQDD221 pKa = 3.28LTFAFGGLANVGVLGDD237 pKa = 3.83YY238 pKa = 10.46QVVVQKK244 pKa = 10.2WDD246 pKa = 3.3ALTGQWTSVDD256 pKa = 3.36GSATGGSILNIGLLDD271 pKa = 3.97GGANGVVIPDD281 pKa = 3.67MDD283 pKa = 4.29AGQYY287 pKa = 9.26RR288 pKa = 11.84AFMVYY293 pKa = 10.29NGVGLSVLTTMTVSGLDD310 pKa = 3.0HH311 pKa = 7.63DD312 pKa = 5.48YY313 pKa = 11.24INTSIVTGEE322 pKa = 3.84ATGNVLDD329 pKa = 4.49NDD331 pKa = 4.19GGAAAAGHH339 pKa = 6.48IVTSVNFNGTDD350 pKa = 3.54YY351 pKa = 11.0PVAAGPAGTTIIGQYY366 pKa = 8.35GQLVIHH372 pKa = 6.45QDD374 pKa = 2.76GSYY377 pKa = 9.95TYY379 pKa = 9.99TPNADD384 pKa = 2.75GTAIGKK390 pKa = 9.2VDD392 pKa = 3.34QFTYY396 pKa = 10.15TLHH399 pKa = 7.55DD400 pKa = 3.7PVANTNTQAILYY412 pKa = 9.18VRR414 pKa = 11.84IDD416 pKa = 3.21SDD418 pKa = 4.12GQGGLVWNDD427 pKa = 2.86ADD429 pKa = 4.25LGADD433 pKa = 3.21ATYY436 pKa = 11.1SVAATNDD443 pKa = 2.99ATEE446 pKa = 5.42IDD448 pKa = 3.92ITWINPTDD456 pKa = 3.26TAYY459 pKa = 10.77FDD461 pKa = 3.5QAQPLIGGALGTVTANSNQFTIGSNMDD488 pKa = 3.03ATGSVVVSVTAAALASGTVTIQKK511 pKa = 10.18LVGSVWQDD519 pKa = 3.11VNTDD523 pKa = 3.01NAFNVAVGVLGNVKK537 pKa = 9.72TIDD540 pKa = 3.55IGSLDD545 pKa = 4.5LDD547 pKa = 3.85AGTYY551 pKa = 9.28RR552 pKa = 11.84VHH554 pKa = 5.79TTLSGALAAISITTDD569 pKa = 3.08VNVTHH574 pKa = 7.47LDD576 pKa = 3.25QHH578 pKa = 6.89VISGDD583 pKa = 3.57PSISGSLVANDD594 pKa = 3.72GVVPEE599 pKa = 4.44GSRR602 pKa = 11.84VVVSDD607 pKa = 3.4GGTFVDD613 pKa = 5.34ASAAGTVIHH622 pKa = 6.66GVHH625 pKa = 6.88GDD627 pKa = 3.31LTVYY631 pKa = 11.23SNGTYY636 pKa = 9.87KK637 pKa = 10.75YY638 pKa = 10.47EE639 pKa = 4.35PLDD642 pKa = 3.44TLAYY646 pKa = 10.03ADD648 pKa = 4.86RR649 pKa = 11.84EE650 pKa = 4.57GTDD653 pKa = 2.86SFTYY657 pKa = 10.27KK658 pKa = 9.98IVLPGGYY665 pKa = 7.54EE666 pKa = 3.87TTAEE670 pKa = 4.31LVVQLHH676 pKa = 7.13DD677 pKa = 3.7GAAVTFTTEE686 pKa = 3.46TADD689 pKa = 3.64PVSAEE694 pKa = 4.25SFSADD699 pKa = 3.0TSGDD703 pKa = 3.56VVALGGLAGSDD714 pKa = 3.76ASDD717 pKa = 3.73SSPEE721 pKa = 3.91PAQHH725 pKa = 6.31NLSEE729 pKa = 4.86ALLLDD734 pKa = 4.41DD735 pKa = 5.08GGGEE739 pKa = 4.05IVLPSSDD746 pKa = 3.93SEE748 pKa = 4.53TGGGSSADD756 pKa = 3.4AAVSSTDD763 pKa = 2.98TDD765 pKa = 5.18LITVDD770 pKa = 3.81EE771 pKa = 5.05GSTVIDD777 pKa = 3.64PLAYY781 pKa = 8.42LTPDD785 pKa = 3.96PLRR788 pKa = 11.84QEE790 pKa = 4.9DD791 pKa = 4.57PPPHH795 pKa = 6.25SAHH798 pKa = 5.81MVV800 pKa = 3.15

Molecular weight: 80.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U4V474|U4V474_9RHIZ Uncharacterized protein OS=Ochrobactrum intermedium 229E OX=1337887 GN=Q644_24875 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.8IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATTGGRR28 pKa = 11.84KK29 pKa = 8.98VLAARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.96RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4249

0

4249

1077194

29

1622

253.5

27.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.356 ± 0.045

0.812 ± 0.012

5.563 ± 0.032

5.847 ± 0.041

3.979 ± 0.028

8.367 ± 0.04

1.989 ± 0.018

5.849 ± 0.031

4.032 ± 0.032

9.851 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.015

3.074 ± 0.022

4.943 ± 0.026

3.222 ± 0.02

6.577 ± 0.044

5.804 ± 0.028

5.224 ± 0.024

7.219 ± 0.033

1.278 ± 0.016

2.379 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski