Methyloglobulus morosus KoM1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methyloglobulus; Methyloglobulus morosus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5DYI1|V5DYI1_9GAMM CoA-binding domain-containing protein OS=Methyloglobulus morosus KoM1 OX=1116472 GN=MGMO_59c00010 PE=4 SV=1
MM1 pKa = 7.36AFVVTEE7 pKa = 3.89NCIKK11 pKa = 10.76CKK13 pKa = 9.68FTDD16 pKa = 4.08CVDD19 pKa = 3.58VCPVDD24 pKa = 5.32CFHH27 pKa = 6.91QGPNFLVIDD36 pKa = 4.26PDD38 pKa = 3.64EE39 pKa = 6.0CIDD42 pKa = 3.79CTLCEE47 pKa = 4.44PEE49 pKa = 4.96CPANAIFAEE58 pKa = 4.35DD59 pKa = 4.05EE60 pKa = 4.27VPEE63 pKa = 4.31GQEE66 pKa = 4.72QFIQLNAEE74 pKa = 4.3LSKK77 pKa = 10.36LWPVITEE84 pKa = 4.09VTKK87 pKa = 10.15PLPDD91 pKa = 4.33ADD93 pKa = 4.18DD94 pKa = 3.87WNGKK98 pKa = 8.22PGKK101 pKa = 10.49LEE103 pKa = 4.15LLEE106 pKa = 4.33KK107 pKa = 10.79

Molecular weight: 11.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5C8Z5|V5C8Z5_9GAMM Methyltransferase type 11 OS=Methyloglobulus morosus KoM1 OX=1116472 GN=MGMO_30c00130 PE=4 SV=1
MM1 pKa = 7.48FGRR4 pKa = 11.84SKK6 pKa = 11.1LLLCRR11 pKa = 11.84YY12 pKa = 5.55VTCRR16 pKa = 11.84RR17 pKa = 11.84PFIFEE22 pKa = 4.26TICEE26 pKa = 4.17VNMKK30 pKa = 10.27NVLSRR35 pKa = 11.84LLVSQAGLEE44 pKa = 4.03ALALRR49 pKa = 11.84LPVGLIRR56 pKa = 11.84AAHH59 pKa = 6.64GSQKK63 pKa = 10.76LFGGG67 pKa = 4.02

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3732

0

3732

1172009

29

4526

314.0

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.782 ± 0.045

1.059 ± 0.014

5.467 ± 0.027

5.807 ± 0.039

4.146 ± 0.026

7.116 ± 0.04

2.297 ± 0.021

6.597 ± 0.032

5.484 ± 0.036

10.608 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.02

4.359 ± 0.033

4.43 ± 0.026

4.258 ± 0.027

4.799 ± 0.032

6.102 ± 0.031

5.423 ± 0.036

6.611 ± 0.031

1.282 ± 0.017

2.987 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski