Grapevine leafroll-associated virus 13

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160NTL8|A0A160NTL8_9CLOS 6 kDa protein OS=Grapevine leafroll-associated virus 13 OX=1815581 GN=p6 PE=4 SV=1
MM1 pKa = 7.45EE2 pKa = 5.04AFIVNFLSMFAAGLYY17 pKa = 9.04STDD20 pKa = 2.82SSFNISEE27 pKa = 4.12MTTLVGIMLPMMASEE42 pKa = 4.49TMRR45 pKa = 11.84SYY47 pKa = 11.45LVLYY51 pKa = 10.13KK52 pKa = 10.41FNKK55 pKa = 8.87LMKK58 pKa = 9.4RR59 pKa = 11.84VSASIPRR66 pKa = 11.84EE67 pKa = 3.78IVEE70 pKa = 4.22SMARR74 pKa = 11.84SIDD77 pKa = 3.54YY78 pKa = 10.5EE79 pKa = 4.29RR80 pKa = 11.84ACNAATHH87 pKa = 6.19HH88 pKa = 6.35FVLGCDD94 pKa = 3.53AISRR98 pKa = 11.84KK99 pKa = 9.37ILLSSTDD106 pKa = 3.56MLSTAVEE113 pKa = 4.53SFTMTSQGLPDD124 pKa = 4.23YY125 pKa = 10.89DD126 pKa = 4.73SLFQTVRR133 pKa = 11.84QTFGLNLEE141 pKa = 4.35PSANLMSLCMQLAEE155 pKa = 4.31DD156 pKa = 3.71VSNNRR161 pKa = 11.84RR162 pKa = 11.84SHH164 pKa = 6.32IAQTRR169 pKa = 11.84NEE171 pKa = 3.73IRR173 pKa = 11.84QCLNEE178 pKa = 4.09RR179 pKa = 11.84NINNHH184 pKa = 4.93ISYY187 pKa = 10.09IGMDD191 pKa = 3.21ASVYY195 pKa = 10.89AEE197 pKa = 3.96WLVDD201 pKa = 3.65LL202 pKa = 5.1

Molecular weight: 22.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A169MUR8|A0A169MUR8_9CLOS Isoform of A0A160NTM9 Putative transmembrane protein OS=Grapevine leafroll-associated virus 13 OX=1815581 GN=p7 PE=4 SV=1
MM1 pKa = 7.31NRR3 pKa = 11.84RR4 pKa = 11.84AFDD7 pKa = 3.36SDD9 pKa = 3.09LRR11 pKa = 11.84AIAVFLIAVASVGLISGGIFLIYY34 pKa = 10.43KK35 pKa = 9.75LRR37 pKa = 11.84NKK39 pKa = 10.3RR40 pKa = 11.84LVDD43 pKa = 3.55KK44 pKa = 10.68EE45 pKa = 3.99SSDD48 pKa = 3.43RR49 pKa = 11.84SKK51 pKa = 11.1SGNVYY56 pKa = 10.27RR57 pKa = 11.84QFPVV61 pKa = 2.71

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

1

12

5171

53

2194

430.9

47.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.175 ± 0.403

1.586 ± 0.203

5.434 ± 0.552

5.763 ± 0.403

4.042 ± 0.328

6.362 ± 0.41

1.682 ± 0.245

6.014 ± 0.425

6.13 ± 0.385

8.528 ± 0.779

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.275

4.738 ± 0.28

3.771 ± 0.269

2.707 ± 0.271

5.86 ± 0.288

9.012 ± 0.247

6.053 ± 0.264

8.026 ± 0.358

0.812 ± 0.076

3.79 ± 0.294
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski