Ryegrass mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9E965|Q9E965_9VIRU p1 OS=Ryegrass mottle virus OX=119910 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.46PSVVIEE7 pKa = 4.09VCSYY11 pKa = 11.46DD12 pKa = 4.55EE13 pKa = 4.13EE14 pKa = 4.82TGDD17 pKa = 5.05CEE19 pKa = 5.52LEE21 pKa = 4.14STSKK25 pKa = 10.52IFTSNFDD32 pKa = 3.39GTYY35 pKa = 10.76VLYY38 pKa = 9.49THH40 pKa = 7.1SSGPKK45 pKa = 7.92YY46 pKa = 10.51AGSTVTLVCPHH57 pKa = 6.79CGVSEE62 pKa = 3.97QATFPTRR69 pKa = 11.84GLSSNWGHH77 pKa = 6.86HH78 pKa = 5.92GPHH81 pKa = 7.54DD82 pKa = 4.9LRR84 pKa = 11.84LDD86 pKa = 3.57CKK88 pKa = 10.38ARR90 pKa = 11.84HH91 pKa = 4.31WHH93 pKa = 5.37GTCEE97 pKa = 4.25VIPSSEE103 pKa = 3.96QEE105 pKa = 3.91SRR107 pKa = 11.84VASDD111 pKa = 4.14GVFEE115 pKa = 5.3PGLTHH120 pKa = 6.3ITTTRR125 pKa = 11.84GQSWTRR131 pKa = 11.84HH132 pKa = 4.41HH133 pKa = 6.93

Molecular weight: 14.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9E965|Q9E965_9VIRU p1 OS=Ryegrass mottle virus OX=119910 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84KK4 pKa = 9.23KK5 pKa = 10.62GKK7 pKa = 9.63SASQVIVLKK16 pKa = 10.56EE17 pKa = 3.66KK18 pKa = 10.56SRR20 pKa = 11.84KK21 pKa = 9.06KK22 pKa = 8.72RR23 pKa = 11.84QKK25 pKa = 10.76SRR27 pKa = 11.84GQQPTRR33 pKa = 11.84QVTPVSAPAAMGTQITYY50 pKa = 10.31RR51 pKa = 11.84GPQVVTQYY59 pKa = 11.97GDD61 pKa = 3.01ITPAKK66 pKa = 10.0NSGSLVRR73 pKa = 11.84VTSSATAGTEE83 pKa = 3.78VSGTVLFNVRR93 pKa = 11.84NATEE97 pKa = 4.5LPWLSGQGSRR107 pKa = 11.84YY108 pKa = 8.08SKK110 pKa = 9.93YY111 pKa = 9.22RR112 pKa = 11.84VRR114 pKa = 11.84YY115 pKa = 9.35AHH117 pKa = 6.1FTWEE121 pKa = 4.77PIVGSNTNGEE131 pKa = 4.21VAMAMLYY138 pKa = 10.52DD139 pKa = 3.82VADD142 pKa = 3.76VTSITIEE149 pKa = 3.74RR150 pKa = 11.84LMQTRR155 pKa = 11.84GGTWGPIWSPTRR167 pKa = 11.84KK168 pKa = 9.5RR169 pKa = 11.84LSYY172 pKa = 10.72DD173 pKa = 3.31PEE175 pKa = 4.49HH176 pKa = 7.25ASLPWYY182 pKa = 10.51LSGVSSGAAAGNIQTPFQIAWAAQSSLVSTTLGRR216 pKa = 11.84IMAEE220 pKa = 3.89YY221 pKa = 10.11LVEE224 pKa = 4.1LTDD227 pKa = 4.37PVDD230 pKa = 3.5VTINQQ235 pKa = 3.25

Molecular weight: 25.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1868

133

947

467.0

50.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.137 ± 0.592

2.141 ± 0.302

4.818 ± 0.45

6.692 ± 0.448

2.141 ± 0.324

8.244 ± 0.078

1.874 ± 0.567

4.39 ± 0.208

4.979 ± 0.505

8.244 ± 0.775

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.767 ± 0.179

2.57 ± 0.113

5.407 ± 0.234

3.64 ± 0.327

6.049 ± 0.47

10.439 ± 0.574

6.263 ± 1.017

7.441 ± 0.473

2.409 ± 0.174

2.355 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski