Flavobacterium sp. ALD4

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; unclassified Flavobacterium

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2796 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N1G3C0|A0A2N1G3C0_9FLAO Orotidine-5'-phosphate decarboxylase OS=Flavobacterium sp. ALD4 OX=2058314 GN=pyrF PE=4 SV=1
MM1 pKa = 7.0KK2 pKa = 10.21TNDD5 pKa = 3.37ILIGEE10 pKa = 4.79DD11 pKa = 3.1GCANLVVGSIVTYY24 pKa = 9.78TFTVTNPGNVSLYY37 pKa = 9.98NVTVADD43 pKa = 3.71PHH45 pKa = 7.25VGLSAIALLSGGDD58 pKa = 3.5ANTNNILEE66 pKa = 4.39VTEE69 pKa = 4.07TWTYY73 pKa = 7.65TATYY77 pKa = 8.98TVTQADD83 pKa = 3.43IDD85 pKa = 3.84EE86 pKa = 4.64GKK88 pKa = 8.39ITNQASVDD96 pKa = 3.5GFAPDD101 pKa = 3.45EE102 pKa = 4.51TKK104 pKa = 10.86VSDD107 pKa = 4.79LSGDD111 pKa = 3.71GANTNEE117 pKa = 4.16EE118 pKa = 4.11NVIPICSSPNIAIVKK133 pKa = 9.21TNDD136 pKa = 2.9ILIGEE141 pKa = 4.78DD142 pKa = 3.38GCVILALGDD151 pKa = 3.7IITYY155 pKa = 8.15TFTVTNPGNVSLHH168 pKa = 5.35NVAVQDD174 pKa = 3.75LHH176 pKa = 6.91PGLSSIALISGVDD189 pKa = 3.33ANTNNILEE197 pKa = 4.36VTEE200 pKa = 3.87TWIYY204 pKa = 9.29RR205 pKa = 11.84ATYY208 pKa = 10.55AVTQADD214 pKa = 3.26IDD216 pKa = 3.78AGKK219 pKa = 8.5ITNQASVDD227 pKa = 3.5GFAPDD232 pKa = 3.45EE233 pKa = 4.51TKK235 pKa = 10.84VSDD238 pKa = 3.87LSGSSEE244 pKa = 4.3TNNDD248 pKa = 2.97ADD250 pKa = 3.98VLIICTTPSITITKK264 pKa = 10.24DD265 pKa = 3.39GIFTDD270 pKa = 3.93STSPTGITNPGDD282 pKa = 3.62TITYY286 pKa = 7.76TFVIEE291 pKa = 4.01NTGNVTLTNVTIADD305 pKa = 4.35PLPGIVITGGPLTSMAAGASNSTTFIGTYY334 pKa = 9.72TITQADD340 pKa = 3.44INAGVVYY347 pKa = 10.76NLATVSGTQPSGTVVTATSTDD368 pKa = 3.55PTPCAICPPKK378 pKa = 9.96PDD380 pKa = 3.92CTGPDD385 pKa = 3.48CANYY389 pKa = 9.36TVTPLPNLKK398 pKa = 8.48VTKK401 pKa = 9.1TATVTTRR408 pKa = 11.84DD409 pKa = 3.88IIDD412 pKa = 3.49EE413 pKa = 4.41VYY415 pKa = 11.08SFVGDD420 pKa = 3.58VINYY424 pKa = 7.99NIRR427 pKa = 11.84VEE429 pKa = 4.07NTGYY433 pKa = 8.46ITILDD438 pKa = 3.8IVVQDD443 pKa = 4.54PLTGLNNTIATLAPGEE459 pKa = 4.14YY460 pKa = 9.68KK461 pKa = 10.68DD462 pKa = 4.5FSEE465 pKa = 4.36SHH467 pKa = 5.79TVTIVDD473 pKa = 4.4LEE475 pKa = 4.22VDD477 pKa = 3.82SVLNIATADD486 pKa = 3.59GFTQTGSPIHH496 pKa = 7.26AEE498 pKa = 3.85DD499 pKa = 3.61SLTVEE504 pKa = 4.32KK505 pKa = 10.89ASVLGCEE512 pKa = 4.86SILVHH517 pKa = 6.66NAFSPNGDD525 pKa = 4.16GINEE529 pKa = 4.04MFVIDD534 pKa = 5.13GIEE537 pKa = 3.75DD538 pKa = 4.3TICYY542 pKa = 9.49PEE544 pKa = 4.16NTVEE548 pKa = 4.81IYY550 pKa = 10.75NRR552 pKa = 11.84WGVLVFEE559 pKa = 4.49TQNYY563 pKa = 8.87NNQTNAFDD571 pKa = 4.4GYY573 pKa = 10.89SRR575 pKa = 11.84GRR577 pKa = 11.84TTIDD581 pKa = 3.17KK582 pKa = 11.04SSGLPTGTYY591 pKa = 9.94FYY593 pKa = 10.24ILKK596 pKa = 8.39YY597 pKa = 9.6TSVDD601 pKa = 3.74LNGNTQANQKK611 pKa = 10.45DD612 pKa = 3.91GYY614 pKa = 11.19LYY616 pKa = 9.48LTKK619 pKa = 10.77

Molecular weight: 65.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N1G7U7|A0A2N1G7U7_9FLAO Mannose-1-phosphate guanylyltransferase OS=Flavobacterium sp. ALD4 OX=2058314 GN=CXF59_06990 PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGYY21 pKa = 9.53DD22 pKa = 4.32AITTDD27 pKa = 3.14KK28 pKa = 10.95PEE30 pKa = 4.34RR31 pKa = 11.84SLIAPIKK38 pKa = 10.47NSGGRR43 pKa = 11.84NSQGKK48 pKa = 6.33MTMRR52 pKa = 11.84YY53 pKa = 7.39TGGGHH58 pKa = 4.86KK59 pKa = 9.44QRR61 pKa = 11.84YY62 pKa = 8.74RR63 pKa = 11.84IIDD66 pKa = 3.78FKK68 pKa = 10.31RR69 pKa = 11.84TKK71 pKa = 10.12EE72 pKa = 4.27GIPATVKK79 pKa = 10.68SIEE82 pKa = 4.0YY83 pKa = 10.15DD84 pKa = 3.43PNRR87 pKa = 11.84TAFIALLAYY96 pKa = 10.31ADD98 pKa = 3.92GEE100 pKa = 4.34KK101 pKa = 10.14TYY103 pKa = 11.26VIAQNGLKK111 pKa = 10.24VGQKK115 pKa = 10.04LLSGPEE121 pKa = 3.96SQPEE125 pKa = 3.92IGNTLPLSRR134 pKa = 11.84IPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAVIARR156 pKa = 11.84SAGTFAQLMARR167 pKa = 11.84DD168 pKa = 4.06GKK170 pKa = 10.75YY171 pKa = 9.05ATIKK175 pKa = 9.6MPSGEE180 pKa = 4.18TRR182 pKa = 11.84LILLTCAATIGAVSNSDD199 pKa = 3.15HH200 pKa = 6.2QLVVSGKK207 pKa = 9.95AGRR210 pKa = 11.84TRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.7PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.29GYY255 pKa = 7.29RR256 pKa = 11.84TRR258 pKa = 11.84SKK260 pKa = 10.85KK261 pKa = 10.4NPSNKK266 pKa = 10.11YY267 pKa = 8.41IVEE270 pKa = 4.07RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2796

0

2796

921015

23

2901

329.4

37.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.7 ± 0.045

0.724 ± 0.014

5.351 ± 0.032

6.361 ± 0.041

5.287 ± 0.037

6.422 ± 0.047

1.661 ± 0.019

8.304 ± 0.049

7.891 ± 0.044

9.31 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.025

6.006 ± 0.046

3.315 ± 0.024

3.295 ± 0.023

3.246 ± 0.027

6.565 ± 0.037

5.866 ± 0.043

6.414 ± 0.04

0.957 ± 0.013

3.962 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski