Helicobacter sp. MIT 14-3879

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; unclassified Helicobacter

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2319 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8I5H4|A0A3D8I5H4_9HELI Flagellar hook-associated protein FlgL OS=Helicobacter sp. MIT 14-3879 OX=2040649 GN=CQA44_10565 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84ISFIDD7 pKa = 3.8ANGHH11 pKa = 3.93TTLWDD16 pKa = 3.17KK17 pKa = 11.9DD18 pKa = 3.97NFVDD22 pKa = 3.75TALGVSANYY31 pKa = 10.43LNEE34 pKa = 3.98TQYY37 pKa = 10.91IIQDD41 pKa = 3.95LYY43 pKa = 10.81FWDD46 pKa = 5.18LIGG49 pKa = 4.91

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8IJW1|A0A3D8IJW1_9HELI Flagellar basal-body rod protein FlgG OS=Helicobacter sp. MIT 14-3879 OX=2040649 GN=flgG PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.07QPHH8 pKa = 5.72NKK10 pKa = 8.48PRR12 pKa = 11.84KK13 pKa = 7.33STHH16 pKa = 4.76GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.77TKK25 pKa = 10.06NGQRR29 pKa = 11.84VIRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.63GRR39 pKa = 11.84KK40 pKa = 8.69RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2319

0

2319

715090

25

2887

308.4

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.698 ± 0.051

1.178 ± 0.019

5.323 ± 0.037

6.12 ± 0.048

5.419 ± 0.049

5.497 ± 0.048

1.637 ± 0.021

10.5 ± 0.063

8.814 ± 0.059

9.957 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.172 ± 0.023

7.227 ± 0.063

2.806 ± 0.028

3.085 ± 0.031

3.317 ± 0.029

7.329 ± 0.039

4.276 ± 0.03

4.87 ± 0.043

0.654 ± 0.016

4.12 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski