Panaeolus cyanescens

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Bolbitiaceae; Panaeolus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13419 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A409Y988|A0A409Y988_9AGAR Uncharacterized protein OS=Panaeolus cyanescens OX=181874 GN=CVT24_005410 PE=4 SV=1
MM1 pKa = 7.12YY2 pKa = 10.17LCLQASCDD10 pKa = 3.92PSDD13 pKa = 3.55MDD15 pKa = 3.68IAVQGVEE22 pKa = 4.75GICEE26 pKa = 3.7NAAISVTLGPADD38 pKa = 3.74VPLTSTSDD46 pKa = 3.45LVTSTDD52 pKa = 3.72TPSSTSSPPSTTTTSTTQSSKK73 pKa = 11.24NNSATSTTSHH83 pKa = 5.99ITSTSTTRR91 pKa = 11.84APSATSSRR99 pKa = 11.84GSARR103 pKa = 11.84GDD105 pKa = 3.08ADD107 pKa = 3.46MCPDD111 pKa = 4.07AMCDD115 pKa = 3.65SEE117 pKa = 6.06SVDD120 pKa = 3.45AANTAVEE127 pKa = 4.47EE128 pKa = 4.38PPPP131 pKa = 4.47

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A409VLR1|A0A409VLR1_9AGAR Uncharacterized protein OS=Panaeolus cyanescens OX=181874 GN=CVT24_011273 PE=4 SV=1
MM1 pKa = 8.75VMMMMGGRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84GRR13 pKa = 11.84TRR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.75GKK19 pKa = 8.43GTGMTTTMMMMKK31 pKa = 9.9RR32 pKa = 11.84RR33 pKa = 11.84GRR35 pKa = 11.84IRR37 pKa = 11.84GRR39 pKa = 11.84RR40 pKa = 11.84IKK42 pKa = 10.51MMIKK46 pKa = 9.98RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84KK50 pKa = 8.72AKK52 pKa = 7.1TVRR55 pKa = 11.84MIKK58 pKa = 8.89KK59 pKa = 6.93TRR61 pKa = 11.84TRR63 pKa = 11.84IRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 5.4TAKK70 pKa = 8.42VRR72 pKa = 11.84RR73 pKa = 11.84MIKK76 pKa = 9.94RR77 pKa = 11.84RR78 pKa = 11.84IKK80 pKa = 10.55RR81 pKa = 11.84MVKK84 pKa = 8.51IRR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84GRR90 pKa = 11.84IGTT93 pKa = 3.76

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13419

0

13419

6793608

16

4760

506.3

56.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.892 ± 0.018

1.171 ± 0.007

5.649 ± 0.015

5.796 ± 0.022

3.799 ± 0.015

6.275 ± 0.018

2.605 ± 0.011

5.137 ± 0.016

4.829 ± 0.017

8.987 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.071 ± 0.007

3.951 ± 0.01

6.594 ± 0.025

3.923 ± 0.012

5.841 ± 0.018

9.027 ± 0.028

6.265 ± 0.014

6.09 ± 0.014

1.373 ± 0.006

2.721 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski