Natrinema pellirubrum (strain DSM 15624 / CIP 106293 / JCM 10476 / NCIMB 786 / 157)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Natrinema; Natrinema pellirubrum

Average proteome isoelectric point is 4.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0JP37|L0JP37_NATP1 Short-chain dehydrogenase/reductase SDR OS=Natrinema pellirubrum (strain DSM 15624 / CIP 106293 / JCM 10476 / NCIMB 786 / 157) OX=797303 GN=Natpe_2819 PE=3 SV=1
MM1 pKa = 7.18TRR3 pKa = 11.84LTRR6 pKa = 11.84RR7 pKa = 11.84STLKK11 pKa = 10.68VAGASLAAATVPATAAAAEE30 pKa = 4.49SDD32 pKa = 4.15DD33 pKa = 3.15GWTVVEE39 pKa = 4.56TPVDD43 pKa = 3.88STLHH47 pKa = 5.97DD48 pKa = 3.61VAYY51 pKa = 8.44TATNPHH57 pKa = 6.12AVAGGGLVIEE67 pKa = 4.73RR68 pKa = 11.84TATGWEE74 pKa = 4.28VVLQGGPTGNGNDD87 pKa = 4.05LYY89 pKa = 11.45GVDD92 pKa = 3.64TTDD95 pKa = 3.47DD96 pKa = 4.38GEE98 pKa = 4.36RR99 pKa = 11.84LWIVGSSGAIGEE111 pKa = 4.31YY112 pKa = 10.65DD113 pKa = 3.62VTTGNLVDD121 pKa = 4.9RR122 pKa = 11.84SAPNDD127 pKa = 3.21YY128 pKa = 8.56TANFNDD134 pKa = 3.71VSVTGPAGEE143 pKa = 4.17ADD145 pKa = 4.12VYY147 pKa = 11.34VADD150 pKa = 4.89DD151 pKa = 4.03SGSIHH156 pKa = 6.94YY157 pKa = 10.87SFDD160 pKa = 3.3NGKK163 pKa = 9.97EE164 pKa = 4.2GTWNYY169 pKa = 8.14EE170 pKa = 4.02VPGSGSGLNAIEE182 pKa = 5.14CYY184 pKa = 10.62ADD186 pKa = 3.66GAGHH190 pKa = 7.34VIDD193 pKa = 4.17TNGKK197 pKa = 9.1VFATDD202 pKa = 5.9DD203 pKa = 3.91GVTWNPIGIEE213 pKa = 4.14DD214 pKa = 4.96AGVTYY219 pKa = 10.92YY220 pKa = 11.4GLDD223 pKa = 3.33SDD225 pKa = 5.77AEE227 pKa = 4.31DD228 pKa = 3.69DD229 pKa = 4.47VYY231 pKa = 11.91VSGGNASVFDD241 pKa = 4.05YY242 pKa = 10.64TGSRR246 pKa = 11.84WIPEE250 pKa = 4.29SLGDD254 pKa = 3.62AALFDD259 pKa = 4.35IEE261 pKa = 4.59TEE263 pKa = 3.83AGTGYY268 pKa = 8.83TVGSGGAVFEE278 pKa = 5.0LEE280 pKa = 4.37DD281 pKa = 4.44GEE283 pKa = 4.61WVQNTTPVGEE293 pKa = 4.25NLRR296 pKa = 11.84AVATGDD302 pKa = 3.02VDD304 pKa = 3.75IVVGSSGSLLEE315 pKa = 4.16RR316 pKa = 4.73

Molecular weight: 32.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0JPU9|L0JPU9_NATP1 Cobalamin B12-binding domain protein OS=Natrinema pellirubrum (strain DSM 15624 / CIP 106293 / JCM 10476 / NCIMB 786 / 157) OX=797303 GN=Natpe_2838 PE=3 SV=1
MM1 pKa = 6.85CTRR4 pKa = 11.84RR5 pKa = 11.84VRR7 pKa = 11.84RR8 pKa = 11.84SAPPLDD14 pKa = 3.73KK15 pKa = 11.05SDD17 pKa = 2.86WFHH20 pKa = 8.5RR21 pKa = 11.84MGTKK25 pKa = 9.99PGQITRR31 pKa = 11.84LLQAFLQNPSHH42 pKa = 7.08LLPVNDD48 pKa = 4.2SSPRR52 pKa = 11.84GYY54 pKa = 9.77WSPVDD59 pKa = 3.27TCRR62 pKa = 11.84RR63 pKa = 11.84FPVEE67 pKa = 3.34EE68 pKa = 4.33SEE70 pKa = 4.5PEE72 pKa = 3.48RR73 pKa = 11.84HH74 pKa = 5.3QPVVVGDD81 pKa = 4.48DD82 pKa = 2.82IDD84 pKa = 4.24RR85 pKa = 11.84PVRR88 pKa = 11.84YY89 pKa = 9.3RR90 pKa = 11.84QDD92 pKa = 2.8HH93 pKa = 6.11SRR95 pKa = 11.84TKK97 pKa = 10.43QEE99 pKa = 3.96EE100 pKa = 4.09QHH102 pKa = 6.9DD103 pKa = 4.16EE104 pKa = 3.97RR105 pKa = 11.84DD106 pKa = 4.23DD107 pKa = 3.47IRR109 pKa = 11.84EE110 pKa = 3.97TLGVHH115 pKa = 5.14TTRR118 pKa = 11.84SATLRR123 pKa = 11.84QKK125 pKa = 10.71RR126 pKa = 11.84GVDD129 pKa = 3.84PVPGSPWGHH138 pKa = 6.79PAHH141 pKa = 6.89LGHH144 pKa = 6.72SGRR147 pKa = 11.84SSDD150 pKa = 3.5PVIFGTVGLEE160 pKa = 4.18TPSKK164 pKa = 10.15RR165 pKa = 11.84FSHH168 pKa = 6.21EE169 pKa = 3.77ARR171 pKa = 11.84PGSAGVAVAIGRR183 pKa = 11.84IHH185 pKa = 6.75GLVHH189 pKa = 6.53TPPKK193 pKa = 9.86SACFRR198 pKa = 11.84LTEE201 pKa = 4.22RR202 pKa = 11.84PRR204 pKa = 11.84FSPTTTGPCGPVVQNSVSSGGSARR228 pKa = 11.84EE229 pKa = 3.57LHH231 pKa = 5.47TRR233 pKa = 11.84CRR235 pKa = 11.84DD236 pKa = 3.08SCVYY240 pKa = 10.58SGIGFWRR247 pKa = 11.84RR248 pKa = 11.84TSSGRR253 pKa = 11.84RR254 pKa = 11.84KK255 pKa = 9.8HH256 pKa = 5.9RR257 pKa = 3.63

Molecular weight: 28.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4138

0

4138

1179910

29

1720

285.1

31.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.877 ± 0.06

0.781 ± 0.013

8.657 ± 0.049

8.869 ± 0.055

3.176 ± 0.025

8.331 ± 0.037

1.946 ± 0.019

4.353 ± 0.029

1.795 ± 0.026

8.954 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.694 ± 0.014

2.284 ± 0.022

4.672 ± 0.026

2.448 ± 0.027

6.651 ± 0.036

5.504 ± 0.028

6.531 ± 0.028

8.604 ± 0.039

1.139 ± 0.015

2.735 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski