Rhodonellum psychrophilum GCM71 = DSM 17998

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Rhodonellum; Rhodonellum psychrophilum

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4904 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5C4Q4|U5C4Q4_9BACT DUF1232 domain-containing protein OS=Rhodonellum psychrophilum GCM71 = DSM 17998 OX=1123057 GN=P872_01105 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.48SPYY5 pKa = 10.71NNINFALIFVGICLISCFININKK28 pKa = 9.67SFSQGFNNNEE38 pKa = 3.78WVFGYY43 pKa = 10.39CGPNDD48 pKa = 3.88PNNYY52 pKa = 10.01LSFGKK57 pKa = 10.44SGDD60 pKa = 3.85PIVRR64 pKa = 11.84SLPGSIVVGANNNALAIDD82 pKa = 5.65PITGQKK88 pKa = 10.71LFFSNGEE95 pKa = 3.75LVYY98 pKa = 10.97NYY100 pKa = 10.83DD101 pKa = 3.71NVPLQGAPNGFNGDD115 pKa = 2.97FDD117 pKa = 3.98GRR119 pKa = 11.84QTVAIGALDD128 pKa = 3.93YY129 pKa = 11.51DD130 pKa = 4.26PDD132 pKa = 4.29GEE134 pKa = 4.25KK135 pKa = 10.65LFYY138 pKa = 10.77AFYY141 pKa = 10.28ISPSGQLLYY150 pKa = 11.07SVVDD154 pKa = 3.6MNAPGGASANQPPLGAVTALDD175 pKa = 3.79QPIGPASGAIAVVKK189 pKa = 8.91TAASPSYY196 pKa = 10.31LISFAGGNLISRR208 pKa = 11.84SIEE211 pKa = 3.83STQGDD216 pKa = 3.96FTLTATQGIPFTPKK230 pKa = 9.96AIVFNEE236 pKa = 4.14STGQLILIPEE246 pKa = 4.13NAIEE250 pKa = 5.12DD251 pKa = 4.22LLLVDD256 pKa = 5.98FDD258 pKa = 4.31TATGSFGAVTPISQSGSAEE277 pKa = 4.16EE278 pKa = 4.08IEE280 pKa = 4.57GASFSPDD287 pKa = 2.63GVFVYY292 pKa = 8.36FSRR295 pKa = 11.84GDD297 pKa = 3.31EE298 pKa = 4.11LLRR301 pKa = 11.84VPVADD306 pKa = 5.11LGATPSVIPFGNDD319 pKa = 2.06IFKK322 pKa = 11.1VYY324 pKa = 10.23DD325 pKa = 3.9LKK327 pKa = 10.91IGPDD331 pKa = 3.09GKK333 pKa = 10.75LYY335 pKa = 10.51YY336 pKa = 10.43LYY338 pKa = 10.7EE339 pKa = 4.26EE340 pKa = 4.56VLGGPQLIGVVNNPNEE356 pKa = 4.11SDD358 pKa = 3.94LDD360 pKa = 4.05LLEE363 pKa = 6.07LDD365 pKa = 4.13EE366 pKa = 6.3DD367 pKa = 4.52PFNGTDD373 pKa = 3.4FCGRR377 pKa = 11.84VFPQFAPNQDD387 pKa = 3.1INPTVDD393 pKa = 4.74FEE395 pKa = 4.91WNPEE399 pKa = 4.16EE400 pKa = 4.39PCSNNPVQLTSLITPEE416 pKa = 3.69NYY418 pKa = 9.92RR419 pKa = 11.84PVSFVWTFEE428 pKa = 3.96PALTDD433 pKa = 3.45EE434 pKa = 5.49DD435 pKa = 4.34GQPVDD440 pKa = 3.07IDD442 pKa = 3.89FNQEE446 pKa = 3.34HH447 pKa = 6.79LLIPEE452 pKa = 4.53EE453 pKa = 4.07ATADD457 pKa = 3.48QSITVTLTVTFADD470 pKa = 4.5GSVVPVTKK478 pKa = 10.32TITLKK483 pKa = 11.12EE484 pKa = 3.93NDD486 pKa = 3.55LEE488 pKa = 4.85ANFSAQDD495 pKa = 3.4TTVCEE500 pKa = 4.58GACVDD505 pKa = 3.6IGSLLEE511 pKa = 3.96VQKK514 pKa = 11.33GGGGDD519 pKa = 3.7GEE521 pKa = 4.39NPNVGGGGGGQQEE534 pKa = 4.42NYY536 pKa = 10.43EE537 pKa = 4.42YY538 pKa = 10.51FWSNRR543 pKa = 11.84RR544 pKa = 11.84GEE546 pKa = 4.28GWVKK550 pKa = 10.86DD551 pKa = 3.68KK552 pKa = 10.97EE553 pKa = 4.41NCVLLPGLYY562 pKa = 8.29WVLVRR567 pKa = 11.84EE568 pKa = 4.43EE569 pKa = 5.13GSDD572 pKa = 3.61CYY574 pKa = 10.94AYY576 pKa = 10.67ASIRR580 pKa = 11.84VKK582 pKa = 10.29IWDD585 pKa = 5.32LPDD588 pKa = 3.02QSNNVWYY595 pKa = 10.11FGDD598 pKa = 4.18GAGLDD603 pKa = 4.31FNPDD607 pKa = 3.68PNDD610 pKa = 3.43PNAPVPRR617 pKa = 11.84PVSHH621 pKa = 7.09PTNIPAGTTTISDD634 pKa = 3.26EE635 pKa = 4.2TGQVLFYY642 pKa = 10.88TDD644 pKa = 5.37GEE646 pKa = 4.97TVWDD650 pKa = 4.2LNGDD654 pKa = 3.65TMANGDD660 pKa = 4.39SIGGSNLATQSVVAVPLPQDD680 pKa = 3.08EE681 pKa = 4.94TIFYY685 pKa = 10.94LFTTQKK691 pKa = 11.15AEE693 pKa = 4.45DD694 pKa = 3.73GTNQVKK700 pKa = 10.21FSLVDD705 pKa = 3.33IKK707 pKa = 11.25VDD709 pKa = 3.31NPTGVGNVVSKK720 pKa = 11.44DD721 pKa = 3.27NFLFSPSTEE730 pKa = 3.65HH731 pKa = 7.25SAGLNSGDD739 pKa = 3.71TTWVIFHH746 pKa = 6.87EE747 pKa = 4.64LGNNTFRR754 pKa = 11.84TYY756 pKa = 10.84PVTTFGIGPPVLSSVGSAHH775 pKa = 6.09TFGSGVGSMKK785 pKa = 10.49FSPDD789 pKa = 2.99GSKK792 pKa = 10.73LAVTIQNGSCSRR804 pKa = 11.84LEE806 pKa = 3.75IFDD809 pKa = 4.55FNQTTGKK816 pKa = 8.1LTEE819 pKa = 4.07YY820 pKa = 11.22ALLEE824 pKa = 4.17LSCSGEE830 pKa = 3.92DD831 pKa = 3.61VYY833 pKa = 11.69GLEE836 pKa = 5.53FSDD839 pKa = 3.72DD840 pKa = 3.73SNRR843 pKa = 11.84VFVSYY848 pKa = 10.11TGGGGKK854 pKa = 8.85IEE856 pKa = 4.27EE857 pKa = 4.25FLIKK861 pKa = 10.94NPDD864 pKa = 3.31AQGAATPSACATCFEE879 pKa = 4.33NAGDD883 pKa = 3.74KK884 pKa = 10.94AAVEE888 pKa = 4.36SCILGTRR895 pKa = 11.84NTLSSAGPFGALQIGPDD912 pKa = 3.55GQIYY916 pKa = 8.59VARR919 pKa = 11.84PGQNVLGTINAGQGCANSAYY939 pKa = 10.72VEE941 pKa = 4.4MGTSTLTGTTNLGLPSFVQNSGSSIPEE968 pKa = 3.9PTLGGPDD975 pKa = 3.94RR976 pKa = 11.84LCLDD980 pKa = 4.05PEE982 pKa = 4.61NGTEE986 pKa = 4.07ALFEE990 pKa = 4.78GGGEE994 pKa = 4.04PDD996 pKa = 2.93IDD998 pKa = 3.99SYY1000 pKa = 11.65FWTIVSEE1007 pKa = 4.0NGQAVLTNFGGPGEE1021 pKa = 4.12EE1022 pKa = 4.4FQTLTHH1028 pKa = 6.38VFQEE1032 pKa = 4.7DD1033 pKa = 4.14GTFTVSLRR1041 pKa = 11.84VDD1043 pKa = 3.03RR1044 pKa = 11.84CGDD1047 pKa = 3.27ANYY1050 pKa = 10.31FEE1052 pKa = 6.26ASMEE1056 pKa = 4.38VVVVAPPPITLVDD1069 pKa = 4.11DD1070 pKa = 3.71VTLCSGTPVTLVAIDD1085 pKa = 4.76GYY1087 pKa = 11.29DD1088 pKa = 3.73PNEE1091 pKa = 3.89GLYY1094 pKa = 10.92DD1095 pKa = 3.87FEE1097 pKa = 4.43WRR1099 pKa = 11.84NAAGQLIGDD1108 pKa = 4.21EE1109 pKa = 4.4NSNSIAVEE1117 pKa = 3.91EE1118 pKa = 4.05EE1119 pKa = 3.92SIYY1122 pKa = 10.76TVTVSYY1128 pKa = 7.6RR1129 pKa = 11.84TPSGSDD1135 pKa = 2.81TVFFDD1140 pKa = 3.25VCPASKK1146 pKa = 10.53SVFVGPAFEE1155 pKa = 5.32FDD1157 pKa = 3.38LTQSAEE1163 pKa = 4.18EE1164 pKa = 4.07VCYY1167 pKa = 11.06DD1168 pKa = 3.28EE1169 pKa = 4.53TLVVFAPNTPVIGDD1183 pKa = 3.91WYY1185 pKa = 7.9YY1186 pKa = 10.82QQVGSPTRR1194 pKa = 11.84TLIGEE1199 pKa = 4.34FFEE1202 pKa = 5.96LEE1204 pKa = 4.9LIPTDD1209 pKa = 4.13LPAPGLYY1216 pKa = 10.06EE1217 pKa = 4.29IIFITEE1223 pKa = 3.89DD1224 pKa = 4.35PIVPGCLVEE1233 pKa = 5.44KK1234 pKa = 10.53KK1235 pKa = 10.74LEE1237 pKa = 4.19LLVHH1241 pKa = 6.46PLPNFEE1247 pKa = 5.53LLVLTDD1253 pKa = 5.08ADD1255 pKa = 4.69DD1256 pKa = 4.23CATPNGSFEE1265 pKa = 3.9ITALLDD1271 pKa = 3.31AEE1273 pKa = 4.52RR1274 pKa = 11.84IEE1276 pKa = 3.99ILEE1279 pKa = 4.17TGEE1282 pKa = 4.0VFNNVNAGDD1291 pKa = 4.06VLPVISNLEE1300 pKa = 3.83PGIYY1304 pKa = 8.88TVQATNSFGCVFTRR1318 pKa = 11.84SVTIANLNPPVALEE1332 pKa = 3.85YY1333 pKa = 10.06TVAVNPEE1340 pKa = 3.73VCGPNEE1346 pKa = 4.04VEE1348 pKa = 4.04NGAVIITFSNGPQSGSYY1365 pKa = 10.71VLTRR1369 pKa = 11.84QQDD1372 pKa = 3.66GLQFSGTITNQEE1384 pKa = 3.72IVTISVPEE1392 pKa = 3.68GDD1394 pKa = 4.01YY1395 pKa = 11.19AVEE1398 pKa = 3.99VSDD1401 pKa = 5.99ALGCTLPEE1409 pKa = 4.0PVNYY1413 pKa = 10.39LVDD1416 pKa = 3.76EE1417 pKa = 4.38KK1418 pKa = 11.48LEE1420 pKa = 4.03VDD1422 pKa = 4.18FTVPTNLIACGLFSFIPQTNQSLVFTLTDD1451 pKa = 3.34ALGNGIAADD1460 pKa = 3.74ANGEE1464 pKa = 4.17FTLLASGAYY1473 pKa = 9.49QITGEE1478 pKa = 4.72DD1479 pKa = 3.91PTGIDD1484 pKa = 3.79CPRR1487 pKa = 11.84VRR1489 pKa = 11.84TMNVEE1494 pKa = 3.69INGPIGFSLSGPADD1508 pKa = 3.53PCATAISYY1516 pKa = 9.99DD1517 pKa = 3.87AILNGTDD1524 pKa = 3.32PADD1527 pKa = 4.05VFFFWKK1533 pKa = 10.16NEE1535 pKa = 3.55SGAIVGRR1542 pKa = 11.84NQTFFPAAAGEE1553 pKa = 4.19YY1554 pKa = 9.7SLEE1557 pKa = 4.08VQPRR1561 pKa = 11.84AGSLCPAAPIDD1572 pKa = 3.79FTVEE1576 pKa = 4.66DD1577 pKa = 4.66FGQPVNVVLDD1587 pKa = 3.99ALPFCGDD1594 pKa = 4.08DD1595 pKa = 3.44PFTTITVVADD1605 pKa = 3.68LTNVASIQWFSLQGGVATLLPGFDD1629 pKa = 4.16DD1630 pKa = 3.63QQTITVIEE1638 pKa = 4.2NGVYY1642 pKa = 9.85QAVLFSGFGCEE1653 pKa = 5.02LGRR1656 pKa = 11.84QQVQITKK1663 pKa = 10.58SFITPPVLEE1672 pKa = 4.16TRR1674 pKa = 11.84YY1675 pKa = 10.0VICAAEE1681 pKa = 4.05NVVNVLDD1688 pKa = 4.13PGVYY1692 pKa = 10.26DD1693 pKa = 3.5NYY1695 pKa = 10.53SWEE1698 pKa = 4.22LEE1700 pKa = 4.0GEE1702 pKa = 4.35EE1703 pKa = 4.53VSNSPTFTPTLPGNYY1718 pKa = 9.51ALTVSDD1724 pKa = 5.19EE1725 pKa = 4.22FDD1727 pKa = 3.53CLFTIEE1733 pKa = 4.76FVVEE1737 pKa = 4.08EE1738 pKa = 4.23DD1739 pKa = 4.31CEE1741 pKa = 4.3LKK1743 pKa = 10.56IIFPNAMRR1751 pKa = 11.84PSDD1754 pKa = 3.9PSRR1757 pKa = 11.84LFVVYY1762 pKa = 10.7ANDD1765 pKa = 4.52FIDD1768 pKa = 4.31HH1769 pKa = 6.34VDD1771 pKa = 3.35VLIYY1775 pKa = 10.61NRR1777 pKa = 11.84WGEE1780 pKa = 4.16LIYY1783 pKa = 10.7FCEE1786 pKa = 4.33SEE1788 pKa = 4.2NVQGEE1793 pKa = 4.47EE1794 pKa = 5.18VLCTWDD1800 pKa = 3.35GTVGGRR1806 pKa = 11.84VVPIGTYY1813 pKa = 9.11PVIVRR1818 pKa = 11.84FRR1820 pKa = 11.84SEE1822 pKa = 3.57NQNIVKK1828 pKa = 8.94TIRR1831 pKa = 11.84KK1832 pKa = 8.59SIVVIEE1838 pKa = 4.1

Molecular weight: 197.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5C5E6|U5C5E6_9BACT 50S ribosomal protein L9 OS=Rhodonellum psychrophilum GCM71 = DSM 17998 OX=1123057 GN=rplI PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.5HH16 pKa = 3.99GFRR19 pKa = 11.84EE20 pKa = 4.18RR21 pKa = 11.84MSTANGRR28 pKa = 11.84RR29 pKa = 11.84VIKK32 pKa = 10.36ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84HH40 pKa = 5.24KK41 pKa = 10.87LSVSSEE47 pKa = 3.97KK48 pKa = 9.91TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4904

0

4904

1499400

29

3651

305.8

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.442 ± 0.037

0.67 ± 0.012

5.222 ± 0.022

6.733 ± 0.034

5.667 ± 0.033

7.143 ± 0.041

1.845 ± 0.019

7.545 ± 0.038

7.123 ± 0.043

9.816 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.018

5.396 ± 0.034

3.893 ± 0.024

3.601 ± 0.019

3.926 ± 0.024

6.545 ± 0.03

5.03 ± 0.037

6.075 ± 0.027

1.219 ± 0.016

3.586 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski