Massilia sp. BSC265

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia; unclassified Massilia

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A086WAN5|A0A086WAN5_9BURK Beta-lactamase OS=Massilia sp. BSC265 OX=1549812 GN=JN27_14330 PE=4 SV=1
MM1 pKa = 7.25TSSDD5 pKa = 3.34FLLRR9 pKa = 11.84YY10 pKa = 10.0ASVTTALFDD19 pKa = 5.08HH20 pKa = 7.15IEE22 pKa = 4.32HH23 pKa = 7.44DD24 pKa = 4.27MLALSCAFIDD34 pKa = 5.79DD35 pKa = 5.33DD36 pKa = 4.81DD37 pKa = 4.51TPPPAWQDD45 pKa = 3.35ALPPHH50 pKa = 7.19AMQPLGLPQAA60 pKa = 4.4

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A086W9M8|A0A086W9M8_9BURK Uroporphyrin-III methyltransferase OS=Massilia sp. BSC265 OX=1549812 GN=JN27_16070 PE=4 SV=1
MM1 pKa = 7.45NEE3 pKa = 3.44HH4 pKa = 6.29AALRR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 4.38RR11 pKa = 11.84NFTWLMSGAVISALGDD27 pKa = 3.56QFSMIALPWLVLQLTGDD44 pKa = 4.02PLALGMMVALMGVPRR59 pKa = 11.84AILILFGGAVVDD71 pKa = 4.79RR72 pKa = 11.84YY73 pKa = 10.54SPKK76 pKa = 10.12RR77 pKa = 11.84VLMLTKK83 pKa = 10.25HH84 pKa = 5.33VNAVLLAILAALVLLGKK101 pKa = 9.1ATLLPVALLAVGLSVASAFSIPAGTSMLPHH131 pKa = 6.56AVAPRR136 pKa = 11.84HH137 pKa = 5.46LAAANAAMMAVRR149 pKa = 11.84QLSLLAGPLLAGLLFVLAGDD169 pKa = 4.84GSRR172 pKa = 11.84GMQDD176 pKa = 2.65AGGLGIAFAVDD187 pKa = 3.59CLSFVLSAWTLSKK200 pKa = 10.57VRR202 pKa = 11.84PRR204 pKa = 11.84VAAEE208 pKa = 3.72ADD210 pKa = 3.24KK211 pKa = 11.12AAAGSVLRR219 pKa = 11.84AVGAGLQALWRR230 pKa = 11.84NPLLRR235 pKa = 11.84TAFLYY240 pKa = 9.85WGLCACVVGGVMQVALPLLASEE262 pKa = 4.7RR263 pKa = 11.84LHH265 pKa = 6.29GASGLALLMGAHH277 pKa = 6.07GTGSLAGMALFGALGRR293 pKa = 11.84RR294 pKa = 11.84RR295 pKa = 11.84VVNLGVTLLLVDD307 pKa = 4.17ALVGVLLLPMGLVTQAWQGVLLLVAIGVLGGFVQVAVFTWIQQCVPQAMLGRR359 pKa = 11.84MMGIFMFVFMGLAPLAAAVAGWLATLLSLASLFAAVGLFLALSAVLAWLFTPMATLTDD417 pKa = 3.94PALPAQHH424 pKa = 6.3QGG426 pKa = 3.06

Molecular weight: 44.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4034

0

4034

1350610

49

1769

334.8

36.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.436 ± 0.058

0.846 ± 0.011

5.335 ± 0.027

5.562 ± 0.04

3.603 ± 0.024

8.464 ± 0.039

2.193 ± 0.017

4.549 ± 0.03

3.522 ± 0.033

10.386 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.02

2.929 ± 0.029

5.14 ± 0.025

3.735 ± 0.026

6.925 ± 0.031

5.391 ± 0.027

5.1 ± 0.026

7.502 ± 0.032

1.326 ± 0.018

2.49 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski