Polaribacter sp. Hel1_33_78

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter; unclassified Polaribacter

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2741 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2DZJ2|A0A1H2DZJ2_9FLAO Nucleotide-binding universal stress protein UspA family OS=Polaribacter sp. Hel1_33_78 OX=1336804 GN=SAMN04487762_0233 PE=3 SV=1
MM1 pKa = 7.7LKK3 pKa = 9.19TFKK6 pKa = 10.47TIIFLFLFSLVFSCSPDD23 pKa = 2.96EE24 pKa = 4.43KK25 pKa = 11.23VNNDD29 pKa = 3.57RR30 pKa = 11.84DD31 pKa = 3.62DD32 pKa = 4.07DD33 pKa = 4.45QIVNSSDD40 pKa = 3.37NCPDD44 pKa = 3.57TQNEE48 pKa = 4.16DD49 pKa = 3.76QLDD52 pKa = 3.82SDD54 pKa = 4.16NDD56 pKa = 4.92GIGDD60 pKa = 4.4LCDD63 pKa = 5.87DD64 pKa = 5.33DD65 pKa = 6.61DD66 pKa = 6.99DD67 pKa = 5.99NDD69 pKa = 4.3GVLDD73 pKa = 4.05TDD75 pKa = 4.88DD76 pKa = 4.53NCPLIANPAQTDD88 pKa = 3.04VDD90 pKa = 3.8NDD92 pKa = 4.09GKK94 pKa = 11.49GDD96 pKa = 3.62TCDD99 pKa = 4.82NDD101 pKa = 5.91DD102 pKa = 5.54DD103 pKa = 6.61DD104 pKa = 6.49SDD106 pKa = 4.21NDD108 pKa = 4.73GIKK111 pKa = 10.56DD112 pKa = 4.65SEE114 pKa = 4.75DD115 pKa = 3.32NCPNIANPNQEE126 pKa = 4.72DD127 pKa = 4.17EE128 pKa = 4.5NNDD131 pKa = 4.24GIGDD135 pKa = 4.16ACASTNKK142 pKa = 9.27FVCEE146 pKa = 3.66NGIANGYY153 pKa = 7.9PCNDD157 pKa = 3.26YY158 pKa = 11.44DD159 pKa = 5.1LLLNIPLATFSASAGNDD176 pKa = 2.8SWGWTDD182 pKa = 3.13ATNGKK187 pKa = 9.43EE188 pKa = 3.87YY189 pKa = 10.78AIMGLNNGTAFIDD202 pKa = 3.51ITDD205 pKa = 4.03TEE207 pKa = 4.4NPVYY211 pKa = 10.42LGKK214 pKa = 10.83LPTASVNSSWRR225 pKa = 11.84DD226 pKa = 2.96IKK228 pKa = 11.06VYY230 pKa = 10.21KK231 pKa = 10.5DD232 pKa = 2.88HH233 pKa = 7.13AFIVSEE239 pKa = 4.02ASNHH243 pKa = 4.45GMQVFDD249 pKa = 3.68LTRR252 pKa = 11.84LRR254 pKa = 11.84NVSNTPATFTADD266 pKa = 3.2TNFTEE271 pKa = 4.91FGKK274 pKa = 10.64AHH276 pKa = 6.61NIVINEE282 pKa = 4.1TSGYY286 pKa = 9.95AYY288 pKa = 9.69IVGASGNSTYY298 pKa = 11.12GGGPIFINIQDD309 pKa = 3.79PKK311 pKa = 10.92NPVSEE316 pKa = 4.56GGFSEE321 pKa = 4.91GGYY324 pKa = 9.66SHH326 pKa = 7.9DD327 pKa = 4.0AQVITYY333 pKa = 8.64NGPDD337 pKa = 3.16TDD339 pKa = 4.06YY340 pKa = 10.45TGKK343 pKa = 10.72EE344 pKa = 3.49ILIGSNEE351 pKa = 4.12NEE353 pKa = 4.15VVIADD358 pKa = 3.82ITDD361 pKa = 3.35KK362 pKa = 8.51TTPTIISTISYY373 pKa = 10.52SNVAYY378 pKa = 10.23AHH380 pKa = 5.84QGWFTEE386 pKa = 3.98DD387 pKa = 2.81LKK389 pKa = 11.63YY390 pKa = 10.73FILGDD395 pKa = 3.79EE396 pKa = 4.18LDD398 pKa = 4.19EE399 pKa = 6.09RR400 pKa = 11.84DD401 pKa = 3.7FGNNTRR407 pKa = 11.84SIIFDD412 pKa = 3.52FTDD415 pKa = 3.74LDD417 pKa = 4.07NPSHH421 pKa = 6.49HH422 pKa = 7.08FDD424 pKa = 3.66YY425 pKa = 10.99FGNTAAIDD433 pKa = 3.38HH434 pKa = 6.38NGYY437 pKa = 10.07VKK439 pKa = 10.8EE440 pKa = 4.13NIYY443 pKa = 10.53YY444 pKa = 8.55QANYY448 pKa = 8.03TAGVRR453 pKa = 11.84MIDD456 pKa = 4.34VSNGDD461 pKa = 3.11NKK463 pKa = 11.08DD464 pKa = 3.62FNEE467 pKa = 3.96VGFFDD472 pKa = 4.92TYY474 pKa = 11.3PNNNNTAFNGVWNVYY489 pKa = 10.19PYY491 pKa = 10.29FPSGNIIISDD501 pKa = 3.38IDD503 pKa = 3.34NGFFVIKK510 pKa = 10.25KK511 pKa = 9.81SNPP514 pKa = 3.15

Molecular weight: 56.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2ESI0|A0A1H2ESI0_9FLAO CarboxypepD_reg-like domain-containing protein OS=Polaribacter sp. Hel1_33_78 OX=1336804 GN=SAMN04487762_1060 PE=4 SV=1
MM1 pKa = 7.2NFIHH5 pKa = 7.2AVRR8 pKa = 11.84HH9 pKa = 4.49FFEE12 pKa = 4.16RR13 pKa = 11.84HH14 pKa = 4.23GFGVFSRR21 pKa = 11.84FADD24 pKa = 3.64KK25 pKa = 11.22LGMRR29 pKa = 11.84AKK31 pKa = 10.16NVRR34 pKa = 11.84IFFIYY39 pKa = 10.2ISFVTVGLSFGLYY52 pKa = 8.32LTLAFLFRR60 pKa = 11.84LKK62 pKa = 11.25DD63 pKa = 3.32MIYY66 pKa = 9.31TKK68 pKa = 10.37RR69 pKa = 11.84SSVFDD74 pKa = 3.68LL75 pKa = 4.35

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2741

0

2741

952477

29

6769

347.5

39.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.071 ± 0.045

0.705 ± 0.014

5.34 ± 0.046

6.446 ± 0.051

5.527 ± 0.048

6.319 ± 0.054

1.693 ± 0.021

8.641 ± 0.05

8.696 ± 0.069

9.086 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.028

6.619 ± 0.053

3.111 ± 0.022

3.175 ± 0.028

3.177 ± 0.028

6.66 ± 0.045

5.809 ± 0.079

5.951 ± 0.036

1.028 ± 0.017

3.855 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski