Stackebrandtia nassauensis (strain DSM 44728 / CIP 108903 / NRRL B-16338 / NBRC 102104 / LLR-40K-21)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Glycomycetales; Glycomycetaceae; Stackebrandtia; Stackebrandtia nassauensis

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6379 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3PWW4|D3PWW4_STANL Monooxygenase FAD-binding protein OS=Stackebrandtia nassauensis (strain DSM 44728 / CIP 108903 / NRRL B-16338 / NBRC 102104 / LLR-40K-21) OX=446470 GN=Snas_5557 PE=4 SV=1
MM1 pKa = 6.7TTHH4 pKa = 7.09HH5 pKa = 7.56DD6 pKa = 3.18IDD8 pKa = 3.46AHH10 pKa = 6.67YY11 pKa = 8.77DD12 pKa = 3.25TDD14 pKa = 5.13DD15 pKa = 3.98PVLLADD21 pKa = 4.49LMARR25 pKa = 11.84VDD27 pKa = 5.32DD28 pKa = 4.8LDD30 pKa = 3.66SHH32 pKa = 6.34QLADD36 pKa = 3.63YY37 pKa = 10.79AIGRR41 pKa = 11.84GLTPPGYY48 pKa = 10.8VPGHH52 pKa = 6.2YY53 pKa = 10.27YY54 pKa = 10.67SIDD57 pKa = 3.77LAWPPGADD65 pKa = 4.02DD66 pKa = 4.75GDD68 pKa = 4.73GVLLWSTSRR77 pKa = 11.84LGDD80 pKa = 3.77DD81 pKa = 4.37GVPADD86 pKa = 4.66EE87 pKa = 5.98DD88 pKa = 4.07DD89 pKa = 3.98AAVWDD94 pKa = 4.21SAQPP98 pKa = 3.41

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3Q5V1|D3Q5V1_STANL Transcriptional regulator LuxR family OS=Stackebrandtia nassauensis (strain DSM 44728 / CIP 108903 / NRRL B-16338 / NBRC 102104 / LLR-40K-21) OX=446470 GN=Snas_0536 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84QGRR40 pKa = 11.84AKK42 pKa = 9.59LTAA45 pKa = 4.17

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6379

0

6379

2067818

30

3291

324.2

34.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.671 ± 0.041

0.801 ± 0.008

6.652 ± 0.03

5.691 ± 0.026

2.965 ± 0.018

8.794 ± 0.032

2.206 ± 0.016

3.969 ± 0.018

2.68 ± 0.03

10.069 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.927 ± 0.014

2.188 ± 0.015

5.621 ± 0.027

2.643 ± 0.015

7.343 ± 0.034

5.468 ± 0.023

6.261 ± 0.024

8.309 ± 0.025

1.576 ± 0.013

2.166 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski