Gillisia limnaea DSM 15749

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Gillisia; Gillisia limnaea

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3411 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2BRS0|H2BRS0_9FLAO Uncharacterized protein OS=Gillisia limnaea DSM 15749 OX=865937 GN=Gilli_0674 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.4KK3 pKa = 10.42LLLLPFIGLCLTACSVDD20 pKa = 3.99PIEE23 pKa = 4.98DD24 pKa = 3.5QIIGEE29 pKa = 4.62NINEE33 pKa = 3.92VNAALVDD40 pKa = 3.92YY41 pKa = 11.01GCAGPDD47 pKa = 2.94NSMTITYY54 pKa = 9.87SEE56 pKa = 4.0AAAIEE61 pKa = 4.26SWDD64 pKa = 3.87EE65 pKa = 3.72VRR67 pKa = 11.84KK68 pKa = 10.19LYY70 pKa = 10.93LSLLAPGVLRR80 pKa = 11.84NGTFDD85 pKa = 3.62PSIWDD90 pKa = 4.42LINRR94 pKa = 11.84FQDD97 pKa = 3.17QGLGDD102 pKa = 3.9YY103 pKa = 9.58TSEE106 pKa = 3.92YY107 pKa = 9.76TIVDD111 pKa = 4.06GEE113 pKa = 4.55CTDD116 pKa = 3.83SVLLTFTVVPDD127 pKa = 4.19GQSEE131 pKa = 4.54PPCEE135 pKa = 4.09PVDD138 pKa = 4.09AGPDD142 pKa = 3.13NSMTITFSEE151 pKa = 4.28AAAIEE156 pKa = 4.3SWDD159 pKa = 3.87EE160 pKa = 3.72VRR162 pKa = 11.84KK163 pKa = 10.19LYY165 pKa = 10.93LSLLAPGVVRR175 pKa = 11.84NGIFDD180 pKa = 3.94PSIWDD185 pKa = 4.3LINRR189 pKa = 11.84FNDD192 pKa = 3.41QEE194 pKa = 4.25NGGPGDD200 pKa = 3.66YY201 pKa = 9.69STEE204 pKa = 3.89YY205 pKa = 10.44TIGDD209 pKa = 4.13GEE211 pKa = 4.57CTDD214 pKa = 3.84SVLLTVTVIPDD225 pKa = 3.56EE226 pKa = 4.23QSNPACAHH234 pKa = 5.56VNAGPDD240 pKa = 3.27KK241 pKa = 10.65SITMTKK247 pKa = 9.56SQAAAIEE254 pKa = 4.28SWDD257 pKa = 3.87EE258 pKa = 3.72VRR260 pKa = 11.84KK261 pKa = 10.19LYY263 pKa = 10.93LSLLAPGVVRR273 pKa = 11.84NGTFDD278 pKa = 3.62PSIWDD283 pKa = 4.3LINRR287 pKa = 11.84FNDD290 pKa = 3.41QEE292 pKa = 4.25NGGPGDD298 pKa = 3.64YY299 pKa = 8.15TTEE302 pKa = 3.83YY303 pKa = 9.95TIVDD307 pKa = 4.38GEE309 pKa = 4.49CSDD312 pKa = 4.61SVLLTITVIPDD323 pKa = 3.02

Molecular weight: 35.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2BWV0|H2BWV0_9FLAO ABC transporter related protein OS=Gillisia limnaea DSM 15749 OX=865937 GN=Gilli_3526 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.05RR21 pKa = 11.84MASVNGRR28 pKa = 11.84KK29 pKa = 9.21VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.62LSVSSEE47 pKa = 4.04TRR49 pKa = 11.84HH50 pKa = 5.02KK51 pKa = 10.46HH52 pKa = 4.65

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3411

0

3411

1128509

30

3739

330.8

37.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.416 ± 0.038

0.666 ± 0.013

5.327 ± 0.032

7.228 ± 0.045

5.362 ± 0.035

6.539 ± 0.044

1.698 ± 0.021

8.053 ± 0.039

7.613 ± 0.053

9.625 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.024

5.837 ± 0.04

3.551 ± 0.026

3.353 ± 0.023

3.703 ± 0.029

6.521 ± 0.031

5.332 ± 0.043

6.013 ± 0.031

1.047 ± 0.016

3.844 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski