Gordonia phage Lucky10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Luckytenvirus; Gordonia virus Lucky10

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142KAZ5|A0A142KAZ5_9CAUD Integrase OS=Gordonia phage Lucky10 OX=1821557 GN=35 PE=3 SV=1
MM1 pKa = 7.51TGIDD5 pKa = 3.38WGGEE9 pKa = 4.12PNWDD13 pKa = 2.89LWRR16 pKa = 11.84WVMSCPNPYY25 pKa = 9.29TGEE28 pKa = 4.26DD29 pKa = 2.64AWQRR33 pKa = 11.84MQRR36 pKa = 11.84EE37 pKa = 4.52AAGEE41 pKa = 3.97PPPRR45 pKa = 11.84LTTPAEE51 pKa = 3.89RR52 pKa = 11.84ATPDD56 pKa = 3.96RR57 pKa = 11.84NPDD60 pKa = 3.41PYY62 pKa = 8.94TASMFRR68 pKa = 11.84AEE70 pKa = 4.97HH71 pKa = 6.63PGLTPGQTCPSCEE84 pKa = 4.61DD85 pKa = 3.81EE86 pKa = 5.17DD87 pKa = 5.69SSIADD92 pKa = 3.17TGVCYY97 pKa = 10.69LCVCGCSYY105 pKa = 11.11CEE107 pKa = 3.95PEE109 pKa = 4.23GRR111 pKa = 4.27

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142KB12|A0A142KB12_9CAUD Uncharacterized protein OS=Gordonia phage Lucky10 OX=1821557 GN=52 PE=4 SV=1
MM1 pKa = 7.55SSSLLEE7 pKa = 4.19GLSDD11 pKa = 3.92LARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84WFRR19 pKa = 11.84WWVMLWQIPSAIVLPMSLLLTHH41 pKa = 6.93WNVALTAAGSIATALFLFALSADD64 pKa = 3.06VDD66 pKa = 3.69QRR68 pKa = 11.84RR69 pKa = 11.84KK70 pKa = 9.59AEE72 pKa = 3.61EE73 pKa = 4.37RR74 pKa = 11.84ITYY77 pKa = 10.01RR78 pKa = 11.84INFDD82 pKa = 2.22VDD84 pKa = 3.08AAMKK88 pKa = 9.42RR89 pKa = 11.84AEE91 pKa = 4.26RR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84MQDD97 pKa = 2.12GGQAA101 pKa = 3.15

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13810

41

1798

197.3

21.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.55 ± 0.539

0.949 ± 0.163

6.915 ± 0.272

5.742 ± 0.358

2.563 ± 0.165

8.581 ± 0.555

2.122 ± 0.203

4.432 ± 0.166

3.157 ± 0.263

7.741 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.157

3.005 ± 0.171

5.72 ± 0.331

3.541 ± 0.184

7.589 ± 0.454

5.894 ± 0.273

7.046 ± 0.331

6.691 ± 0.286

2.281 ± 0.182

2.129 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski