Velvet bean severe mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C9YHF0|C9YHF0_9GEMI Replication enhancer OS=Velvet bean severe mosaic virus OX=667119 GN=AC3 PE=3 SV=1
MM1 pKa = 7.73VLILRR6 pKa = 11.84SFLVVIHH13 pKa = 6.0HH14 pKa = 6.67MIVNTEE20 pKa = 3.78VLVNHH25 pKa = 6.82GLLLTSILATCNSGIEE41 pKa = 4.08SSQYY45 pKa = 10.86LVTITKK51 pKa = 9.81IVFDD55 pKa = 4.13CCSAGFIIIHH65 pKa = 5.52VEE67 pKa = 3.79HH68 pKa = 6.59LTKK71 pKa = 10.21IHH73 pKa = 6.88RR74 pKa = 11.84CSEE77 pKa = 3.96WSSVTTT83 pKa = 4.07

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C9YHF5|C9YHF5_9GEMI Movement protein BC1 OS=Velvet bean severe mosaic virus OX=667119 GN=BC1 PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 9.75MLYY5 pKa = 9.16TPRR8 pKa = 11.84SNWRR12 pKa = 11.84SPFRR16 pKa = 11.84RR17 pKa = 11.84NTSNRR22 pKa = 11.84RR23 pKa = 11.84NVRR26 pKa = 11.84RR27 pKa = 11.84TNSATRR33 pKa = 11.84FNIKK37 pKa = 8.25VARR40 pKa = 11.84RR41 pKa = 11.84LSYY44 pKa = 10.99GRR46 pKa = 11.84VEE48 pKa = 4.6RR49 pKa = 11.84PLQFGKK55 pKa = 10.61LCEE58 pKa = 4.18KK59 pKa = 10.39HH60 pKa = 6.63HH61 pKa = 6.0GVHH64 pKa = 6.45MSLCSNRR71 pKa = 11.84DD72 pKa = 3.12VTSFISYY79 pKa = 8.18PALSLNGDD87 pKa = 3.21GRR89 pKa = 11.84SRR91 pKa = 11.84DD92 pKa = 4.09FIKK95 pKa = 10.82LLSLNISGVLSARR108 pKa = 11.84VVSSDD113 pKa = 3.16QPMDD117 pKa = 3.4NDD119 pKa = 3.58VYY121 pKa = 11.05RR122 pKa = 11.84RR123 pKa = 11.84GLFVISIILDD133 pKa = 3.72RR134 pKa = 11.84KK135 pKa = 10.07PYY137 pKa = 10.48VPDD140 pKa = 3.94GANEE144 pKa = 3.95LPSFEE149 pKa = 4.83EE150 pKa = 4.45LFGQYY155 pKa = 10.21SEE157 pKa = 6.06AYY159 pKa = 9.71VNMRR163 pKa = 11.84LLSSRR168 pKa = 11.84QDD170 pKa = 3.04RR171 pKa = 11.84FRR173 pKa = 11.84LLGTIKK179 pKa = 10.73KK180 pKa = 10.19NINCDD185 pKa = 3.09SGAADD190 pKa = 3.5VNIGKK195 pKa = 9.64FIRR198 pKa = 11.84FVQGRR203 pKa = 11.84RR204 pKa = 11.84TLWSRR209 pKa = 11.84FKK211 pKa = 11.11DD212 pKa = 3.58PEE214 pKa = 4.58PPLDD218 pKa = 3.53SGGNYY223 pKa = 10.08RR224 pKa = 11.84NISTNAILINYY235 pKa = 7.92AFVSMQSITVNPLVQYY251 pKa = 9.24EE252 pKa = 4.16LNYY255 pKa = 10.66VGG257 pKa = 5.28

Molecular weight: 29.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1741

83

362

193.4

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.055 ± 0.788

2.24 ± 0.391

4.825 ± 0.517

5.055 ± 0.583

4.882 ± 0.178

4.48 ± 0.459

2.929 ± 0.489

5.859 ± 0.659

6.203 ± 0.788

7.812 ± 0.863

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.585 ± 0.532

6.72 ± 0.482

4.767 ± 0.547

3.906 ± 0.409

7.122 ± 0.989

8.558 ± 0.869

5.859 ± 0.749

6.031 ± 0.828

1.091 ± 0.088

4.021 ± 0.548
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski