Mycobacterium phage Fancypants

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481VU12|A0A481VU12_9CAUD Uncharacterized protein OS=Mycobacterium phage Fancypants OX=2530128 GN=67 PE=4 SV=1
MM1 pKa = 7.86SDD3 pKa = 4.02RR4 pKa = 11.84IEE6 pKa = 4.16ATLGQMFRR14 pKa = 11.84DD15 pKa = 3.65HH16 pKa = 7.09FFDD19 pKa = 3.47DD20 pKa = 4.98TYY22 pKa = 11.47PEE24 pKa = 5.58DD25 pKa = 3.61EE26 pKa = 4.61TEE28 pKa = 4.26CCVEE32 pKa = 4.18EE33 pKa = 4.83FLATLKK39 pKa = 10.06TNRR42 pKa = 11.84IALVEE47 pKa = 4.03LPEE50 pKa = 4.49KK51 pKa = 10.6LGEE54 pKa = 4.29DD55 pKa = 3.26APYY58 pKa = 10.02WRR60 pKa = 11.84TLIGNEE66 pKa = 4.18PEE68 pKa = 4.49DD69 pKa = 4.45VYY71 pKa = 10.98PSPLGHH77 pKa = 6.23IVISGAAHH85 pKa = 6.67LDD87 pKa = 3.73SVEE90 pKa = 4.04EE91 pKa = 4.1ARR93 pKa = 11.84DD94 pKa = 3.26LAAALLAAAAEE105 pKa = 4.41VTEE108 pKa = 4.3

Molecular weight: 11.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481VU36|A0A481VU36_9CAUD Uncharacterized protein OS=Mycobacterium phage Fancypants OX=2530128 GN=93 PE=4 SV=1
MM1 pKa = 8.05PDD3 pKa = 3.22RR4 pKa = 11.84YY5 pKa = 10.68GDD7 pKa = 3.63PTPEE11 pKa = 3.56PRR13 pKa = 11.84VFVRR17 pKa = 11.84PKK19 pKa = 10.42VNALTVRR26 pKa = 11.84CSWCKK31 pKa = 9.95AGVGSRR37 pKa = 11.84CVVAGTGVVLRR48 pKa = 11.84RR49 pKa = 11.84SSFHH53 pKa = 6.89DD54 pKa = 3.24VRR56 pKa = 11.84IRR58 pKa = 11.84DD59 pKa = 3.76AEE61 pKa = 4.16LAATGALARR70 pKa = 11.84GRR72 pKa = 11.84MSS74 pKa = 3.0

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

19033

30

1175

173.0

18.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.513 ± 0.515

1.25 ± 0.161

6.657 ± 0.214

6.089 ± 0.319

2.932 ± 0.19

8.753 ± 0.579

2.254 ± 0.195

4.403 ± 0.175

3.389 ± 0.179

7.256 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.127

3.336 ± 0.163

5.942 ± 0.189

3.405 ± 0.207

6.935 ± 0.381

5.8 ± 0.277

6.547 ± 0.218

7.35 ± 0.251

2.27 ± 0.145

2.648 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski