Modestobacter multiseptatus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Modestobacter

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5324 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A543CAX5|A0A543CAX5_9ACTN Nicotinamide-nucleotide amidase OS=Modestobacter multiseptatus OX=88139 GN=FB553_3403 PE=4 SV=1
MM1 pKa = 7.3VPATTEE7 pKa = 4.11RR8 pKa = 11.84EE9 pKa = 4.16PMPATSRR16 pKa = 11.84RR17 pKa = 11.84PARR20 pKa = 11.84ALLGGVVAAVVLTGCAPTLVVGSATPAAGQVGEE53 pKa = 4.4VRR55 pKa = 11.84AADD58 pKa = 3.95FPIIGATDD66 pKa = 3.48EE67 pKa = 5.06VVDD70 pKa = 3.92QLARR74 pKa = 11.84NALSDD79 pKa = 4.79LNTYY83 pKa = 7.43WQQQLPDD90 pKa = 3.63TLGTDD95 pKa = 4.22FTGLAGGYY103 pKa = 10.0FSVDD107 pKa = 3.39PANVDD112 pKa = 3.27PAQYY116 pKa = 9.79PGGEE120 pKa = 4.25IGCGEE125 pKa = 4.48PPDD128 pKa = 4.7AVADD132 pKa = 3.73NAFYY136 pKa = 10.75CPPSSEE142 pKa = 4.37YY143 pKa = 10.44PNGDD147 pKa = 3.79AIQYY151 pKa = 9.28DD152 pKa = 3.86RR153 pKa = 11.84SFLRR157 pKa = 11.84EE158 pKa = 3.62LADD161 pKa = 3.99DD162 pKa = 3.86YY163 pKa = 11.9GRR165 pKa = 11.84FLPALVMAHH174 pKa = 6.23EE175 pKa = 5.31FGHH178 pKa = 6.69AVQARR183 pKa = 11.84VGYY186 pKa = 8.75PLQASIAIEE195 pKa = 4.03TQADD199 pKa = 3.98CFAGAWTAWVADD211 pKa = 3.86GNAPHH216 pKa = 5.84TTIRR220 pKa = 11.84TPEE223 pKa = 4.18LDD225 pKa = 3.36EE226 pKa = 4.06VLRR229 pKa = 11.84GYY231 pKa = 10.87SLLRR235 pKa = 11.84DD236 pKa = 3.59PVGTSVNEE244 pKa = 4.28TEE246 pKa = 4.21AHH248 pKa = 6.12GSLFDD253 pKa = 4.09RR254 pKa = 11.84VSAFQDD260 pKa = 3.6GFDD263 pKa = 5.04DD264 pKa = 5.52GPGACRR270 pKa = 11.84DD271 pKa = 3.73DD272 pKa = 4.58FGADD276 pKa = 3.01RR277 pKa = 11.84PYY279 pKa = 10.1TQSEE283 pKa = 4.5FNPAEE288 pKa = 3.95AANNGDD294 pKa = 2.79ASYY297 pKa = 10.58PVTQDD302 pKa = 4.66LITTALPDD310 pKa = 3.9FWTSAFSGLDD320 pKa = 3.29EE321 pKa = 4.25QFQAPVIVPFSGTAPDD337 pKa = 3.68CGGRR341 pKa = 11.84EE342 pKa = 4.16PDD344 pKa = 3.39TDD346 pKa = 4.59LVFCAADD353 pKa = 3.41DD354 pKa = 3.96TVGYY358 pKa = 10.78DD359 pKa = 3.73EE360 pKa = 5.47TDD362 pKa = 3.22LAQPVYY368 pKa = 11.21ADD370 pKa = 3.53LGDD373 pKa = 3.99YY374 pKa = 10.98AVLTAAAIPYY384 pKa = 9.85SLAARR389 pKa = 11.84DD390 pKa = 3.82QLGLSTDD397 pKa = 3.58DD398 pKa = 3.49QDD400 pKa = 4.2ALRR403 pKa = 11.84SAICLTGAFTFAVLRR418 pKa = 11.84GSVSAIQISPGDD430 pKa = 3.41VDD432 pKa = 4.16EE433 pKa = 5.08SVQFLLEE440 pKa = 4.07YY441 pKa = 10.8SDD443 pKa = 5.35DD444 pKa = 3.94PTVLADD450 pKa = 3.68GGLTGFQLVDD460 pKa = 3.73LFRR463 pKa = 11.84SGVFGGLSSCGIGGG477 pKa = 3.51

Molecular weight: 50.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543C4M6|A0A543C4M6_9ACTN Uncharacterized protein OS=Modestobacter multiseptatus OX=88139 GN=FB553_0967 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.25TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5324

0

5324

1567677

29

1939

294.5

31.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.263 ± 0.053

0.747 ± 0.011

6.128 ± 0.028

5.099 ± 0.033

2.517 ± 0.019

9.559 ± 0.032

2.085 ± 0.015

3.004 ± 0.024

1.479 ± 0.021

10.617 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.657 ± 0.012

1.56 ± 0.017

6.161 ± 0.03

2.965 ± 0.021

8.147 ± 0.034

5.097 ± 0.024

6.183 ± 0.03

9.45 ± 0.033

1.498 ± 0.016

1.783 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski