Bat coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Coronavirinae; unclassified Coronavirinae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4EVQ5|A0A2Z4EVQ5_9NIDO ORF3 OS=Bat coronavirus OX=1508220 PE=4 SV=1
MM1 pKa = 7.22MLINLLRR8 pKa = 11.84KK9 pKa = 10.05LYY11 pKa = 10.14GSPAAPQQAISHH23 pKa = 6.55DD24 pKa = 3.81VSIVNAEE31 pKa = 3.95ALEE34 pKa = 4.1PVYY37 pKa = 11.27NMPEE41 pKa = 3.87VEE43 pKa = 4.35ANEE46 pKa = 4.11SLLLFILGSVFYY58 pKa = 10.95VLLSLILWLLSTVNKK73 pKa = 9.58PYY75 pKa = 10.88LSIVSSVIFCFVQLVMVIWFVLCDD99 pKa = 3.58NVLISIVAQCFLAFLFVMCFLEE121 pKa = 4.17RR122 pKa = 11.84SYY124 pKa = 11.55LAFRR128 pKa = 11.84LRR130 pKa = 11.84SLAPFVHH137 pKa = 6.28SCSAFVVINTTSNRR151 pKa = 11.84YY152 pKa = 8.32VFPLQGVAEE161 pKa = 4.28HH162 pKa = 6.36VVILTTNAGVSCNGVFQQGAIAVTDD187 pKa = 3.33TATIYY192 pKa = 10.52TRR194 pKa = 11.84FSSHH198 pKa = 6.12VLLLDD203 pKa = 3.96RR204 pKa = 11.84VEE206 pKa = 4.62HH207 pKa = 6.45GYY209 pKa = 11.03DD210 pKa = 3.18YY211 pKa = 11.3TVFAFVSTVVLEE223 pKa = 3.87NTRR226 pKa = 11.84RR227 pKa = 11.84IGVSTGMTDD236 pKa = 3.36VEE238 pKa = 4.44LL239 pKa = 4.93

Molecular weight: 26.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4EVP6|A0A2Z4EVP6_9NIDO Uncharacterized protein OS=Bat coronavirus OX=1508220 PE=4 SV=1
MM1 pKa = 7.42SGRR4 pKa = 11.84RR5 pKa = 11.84TPRR8 pKa = 11.84NQPQVSFKK16 pKa = 11.16NEE18 pKa = 3.78SDD20 pKa = 3.53SDD22 pKa = 4.25SEE24 pKa = 4.34SGARR28 pKa = 11.84SQSRR32 pKa = 11.84GRR34 pKa = 11.84NSNNNNNGGNGGARR48 pKa = 11.84RR49 pKa = 11.84KK50 pKa = 10.1DD51 pKa = 3.45KK52 pKa = 10.77PEE54 pKa = 3.97KK55 pKa = 9.96PRR57 pKa = 11.84AAPAQNVSWFLPIVQTGKK75 pKa = 9.96QDD77 pKa = 3.19LRR79 pKa = 11.84FARR82 pKa = 11.84GEE84 pKa = 4.16GVPVSQGVDD93 pKa = 2.6ITYY96 pKa = 10.22QHH98 pKa = 6.81GYY100 pKa = 6.01WLRR103 pKa = 11.84RR104 pKa = 11.84QRR106 pKa = 11.84TFNKK110 pKa = 10.11GGKK113 pKa = 7.95QVQANPRR120 pKa = 11.84WFFYY124 pKa = 9.86YY125 pKa = 10.12TGTGPYY131 pKa = 9.53EE132 pKa = 3.84GLRR135 pKa = 11.84YY136 pKa = 9.92GSRR139 pKa = 11.84NNDD142 pKa = 3.71IIWVGNEE149 pKa = 3.46GANVNRR155 pKa = 11.84LGDD158 pKa = 3.38MGTRR162 pKa = 11.84NPANDD167 pKa = 3.39AGIPVQLAEE176 pKa = 5.13GIPKK180 pKa = 10.24GFYY183 pKa = 10.51AEE185 pKa = 4.29GRR187 pKa = 11.84NSRR190 pKa = 11.84GNSRR194 pKa = 11.84NSSRR198 pKa = 11.84SSSRR202 pKa = 11.84GSSNANSRR210 pKa = 11.84NQSRR214 pKa = 11.84SNSPGRR220 pKa = 11.84GSAPPSGGEE229 pKa = 3.31PWMAYY234 pKa = 10.38LIQKK238 pKa = 10.09LEE240 pKa = 4.0NLEE243 pKa = 3.82QRR245 pKa = 11.84VDD247 pKa = 3.43GKK249 pKa = 11.17KK250 pKa = 9.94SDD252 pKa = 3.66KK253 pKa = 10.99QPVKK257 pKa = 8.99VTKK260 pKa = 10.61NVASEE265 pKa = 3.86NAKK268 pKa = 10.25KK269 pKa = 10.25LRR271 pKa = 11.84HH272 pKa = 6.07KK273 pKa = 9.87RR274 pKa = 11.84TAHH277 pKa = 6.33KK278 pKa = 10.56GSNATQNYY286 pKa = 7.8GRR288 pKa = 11.84RR289 pKa = 11.84GPGNLEE295 pKa = 4.0GNFGDD300 pKa = 5.09QEE302 pKa = 4.17FLKK305 pKa = 10.93LGTDD309 pKa = 3.63DD310 pKa = 3.63PRR312 pKa = 11.84FPVVAQMAPNTSSFVFMSHH331 pKa = 5.45FTPRR335 pKa = 11.84YY336 pKa = 8.26EE337 pKa = 6.0ADD339 pKa = 4.35ALWLDD344 pKa = 3.67YY345 pKa = 10.64TGSIKK350 pKa = 10.65LPRR353 pKa = 11.84DD354 pKa = 3.78DD355 pKa = 5.33PNFPQWEE362 pKa = 4.24KK363 pKa = 11.29LLAEE367 pKa = 5.1NIDD370 pKa = 3.92AYY372 pKa = 10.3KK373 pKa = 10.54SFPPPKK379 pKa = 9.59PKK381 pKa = 9.89SDD383 pKa = 3.29KK384 pKa = 10.4KK385 pKa = 10.84KK386 pKa = 10.84KK387 pKa = 9.75SDD389 pKa = 3.95KK390 pKa = 10.35SDD392 pKa = 3.34SAAGPSEE399 pKa = 4.28DD400 pKa = 4.39LQMQVVDD407 pKa = 3.55PSGVQRR413 pKa = 11.84IYY415 pKa = 10.71MKK417 pKa = 10.71DD418 pKa = 3.22AADD421 pKa = 3.57QTDD424 pKa = 4.45DD425 pKa = 3.93EE426 pKa = 4.65WLQDD430 pKa = 3.35DD431 pKa = 5.4TIYY434 pKa = 11.08EE435 pKa = 4.23DD436 pKa = 5.13EE437 pKa = 4.1NDD439 pKa = 3.5KK440 pKa = 11.22PKK442 pKa = 10.71AQRR445 pKa = 11.84RR446 pKa = 11.84QSIKK450 pKa = 10.28KK451 pKa = 10.2RR452 pKa = 11.84NATHH456 pKa = 5.6QRR458 pKa = 11.84HH459 pKa = 4.8VSIDD463 pKa = 3.25GAAQSSAA470 pKa = 3.23

Molecular weight: 52.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

9311

146

4205

1163.9

129.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.421 ± 0.357

2.9 ± 0.312

5.241 ± 0.551

3.684 ± 0.344

5.069 ± 0.386

6.036 ± 0.322

1.751 ± 0.262

4.339 ± 0.249

4.704 ± 0.608

9.344 ± 0.485

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.215

5.091 ± 0.551

4.672 ± 0.276

3.566 ± 0.395

4.189 ± 0.412

7.239 ± 0.674

6.927 ± 0.537

9.612 ± 0.54

1.31 ± 0.138

4.65 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski