Deefgea sp. D17

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Chromobacteriaceae; Deefgea; unclassified Deefgea

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3066 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6M8SNY1|A0A6M8SNY1_9NEIS Response regulator transcription factor OS=Deefgea sp. D17 OX=2739061 GN=HQN60_03565 PE=4 SV=1
MM1 pKa = 6.12TTEE4 pKa = 4.21TEE6 pKa = 4.03MPLPFVFTDD15 pKa = 3.5NAASKK20 pKa = 10.54VNEE23 pKa = 4.78LILEE27 pKa = 4.35EE28 pKa = 4.64GNPDD32 pKa = 2.94LKK34 pKa = 11.14LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.94GFTFDD54 pKa = 4.69EE55 pKa = 4.93IVNEE59 pKa = 4.46DD60 pKa = 3.8DD61 pKa = 4.54TPITKK66 pKa = 10.48NGVTLLVDD74 pKa = 3.69PMSYY78 pKa = 10.34QYY80 pKa = 11.57LVGAEE85 pKa = 3.69IDD87 pKa = 3.85YY88 pKa = 11.28VEE90 pKa = 4.54SLEE93 pKa = 4.67GSQFTIKK100 pKa = 10.63NPNAQSTCGCGSSFSVV116 pKa = 3.54

Molecular weight:
12.59 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6M8SN14|A0A6M8SN14_9NEIS Peptidyl-prolyl cis-trans isomerase OS=Deefgea sp. D17 OX=2739061 GN=HQN60_07465 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.26RR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGVRR29 pKa = 11.84VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.53GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight:
4.99 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3066

0

3066

984057

37

4315

321.0

35.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.089 ± 0.062

0.998 ± 0.014

5.113 ± 0.03

5.37 ± 0.038

3.84 ± 0.034

7.019 ± 0.043

2.212 ± 0.022

6.028 ± 0.038

4.377 ± 0.042

11.163 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.024

3.736 ± 0.035

4.358 ± 0.031

5.015 ± 0.046

5.15 ± 0.043

6.058 ± 0.036

5.067 ± 0.056

6.83 ± 0.043

1.39 ± 0.018

2.598 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski