Pseudomonas phage Skulduggery

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y0SZB0|A0A1Y0SZB0_9CAUD Uncharacterized protein OS=Pseudomonas phage Skulduggery OX=2006671 GN=SKUL_14 PE=4 SV=1
MM1 pKa = 7.54SKK3 pKa = 9.97QPQTLTVGGKK13 pKa = 9.22EE14 pKa = 3.84LVEE17 pKa = 4.62GYY19 pKa = 10.87GFAFVGNYY27 pKa = 9.95FMDD30 pKa = 4.73GKK32 pKa = 9.86HH33 pKa = 5.24GQIGVSGGFDD43 pKa = 3.33EE44 pKa = 4.71EE45 pKa = 4.65TAHH48 pKa = 5.76ITATISILTILGKK61 pKa = 10.6AFDD64 pKa = 4.86RR65 pKa = 11.84NLQSGVPAQPLIEE78 pKa = 5.06DD79 pKa = 3.83PVFTLTIDD87 pKa = 3.92GLPDD91 pKa = 4.0FVSGRR96 pKa = 11.84TEE98 pKa = 4.51PYY100 pKa = 9.02PLKK103 pKa = 10.69AVYY106 pKa = 10.0QLGADD111 pKa = 4.56LAADD115 pKa = 4.31DD116 pKa = 4.27TPGAYY121 pKa = 9.98GEE123 pKa = 5.0LIVDD127 pKa = 3.69AGYY130 pKa = 10.77NYY132 pKa = 10.05EE133 pKa = 3.9RR134 pKa = 11.84AEE136 pKa = 4.43AIGAQRR142 pKa = 11.84IVQQIAAEE150 pKa = 4.12LAAQEE155 pKa = 4.32EE156 pKa = 4.8

Molecular weight:
16.61 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y0SZK2|A0A1Y0SZK2_9CAUD Uncharacterized protein OS=Pseudomonas phage Skulduggery OX=2006671 GN=SKUL_7 PE=4 SV=1
MM1 pKa = 7.14VSAPGRR7 pKa = 11.84RR8 pKa = 11.84DD9 pKa = 4.2DD10 pKa = 4.85IMLKK14 pKa = 8.51IHH16 pKa = 6.57ARR18 pKa = 11.84VEE20 pKa = 4.2VVDD23 pKa = 3.79MGFVLDD29 pKa = 5.24GKK31 pKa = 9.82PSPCHH36 pKa = 6.48LWTGPHH42 pKa = 6.41SGTGRR47 pKa = 11.84GGGYY51 pKa = 10.84GRR53 pKa = 11.84MHH55 pKa = 7.62LSGQTVAVHH64 pKa = 5.89IVVFTHH70 pKa = 6.81FYY72 pKa = 10.82GYY74 pKa = 11.05VPGKK78 pKa = 10.04KK79 pKa = 9.86QIDD82 pKa = 3.91HH83 pKa = 6.47KK84 pKa = 10.93CSNRR88 pKa = 11.84TCCNPAHH95 pKa = 6.27LQMVTHH101 pKa = 6.77KK102 pKa = 10.53QNQRR106 pKa = 11.84LRR108 pKa = 11.84DD109 pKa = 3.5KK110 pKa = 11.04RR111 pKa = 11.84AGAKK115 pKa = 9.54KK116 pKa = 10.36AA117 pKa = 3.38

Molecular weight:
12.94 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

20252

47

3695

281.3

30.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.382 ± 0.474

0.879 ± 0.164

6.197 ± 0.193

6.73 ± 0.284

3.373 ± 0.162

8.72 ± 0.368

1.778 ± 0.12

4.498 ± 0.185

5.555 ± 0.257

7.728 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.147

3.733 ± 0.121

4.8 ± 0.222

4.829 ± 0.217

5.792 ± 0.222

5.16 ± 0.181

6.059 ± 0.243

5.915 ± 0.267

1.358 ± 0.133

2.726 ± 0.139

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski