Shigella phage Sf6 (Shigella flexneri bacteriophage VI) (Bacteriophage SfVI)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q716C5|Q716C5_BPSFV Protein ninE OS=Shigella phage Sf6 OX=10761 GN=52 PE=3 SV=1
MM1 pKa = 7.76IDD3 pKa = 3.19QNRR6 pKa = 11.84SYY8 pKa = 10.32EE9 pKa = 4.03QEE11 pKa = 4.1SVEE14 pKa = 5.1RR15 pKa = 11.84ALTCANCGQKK25 pKa = 10.32LHH27 pKa = 5.86VLEE30 pKa = 4.31VHH32 pKa = 5.94VCEE35 pKa = 4.54HH36 pKa = 6.71CCAEE40 pKa = 4.99LMSDD44 pKa = 4.24PNSSMHH50 pKa = 6.34EE51 pKa = 4.12EE52 pKa = 4.06EE53 pKa = 5.84DD54 pKa = 4.39DD55 pKa = 3.83GG56 pKa = 5.68

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q716D9|Q716D9_BPSFV Gene 36 protein OS=Shigella phage Sf6 OX=10761 GN=36 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 4.41AQTRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 3.77RR10 pKa = 11.84RR11 pKa = 11.84AEE13 pKa = 4.15KK14 pKa = 9.26QAQWKK19 pKa = 8.58AANPLLVGVSAKK31 pKa = 9.96PVNRR35 pKa = 11.84PILSLNRR42 pKa = 11.84KK43 pKa = 7.08PKK45 pKa = 10.54SRR47 pKa = 11.84VEE49 pKa = 4.08SALNPIDD56 pKa = 3.85LTVLAEE62 pKa = 3.84YY63 pKa = 10.59HH64 pKa = 5.65EE65 pKa = 4.71QIEE68 pKa = 4.84SNLQRR73 pKa = 11.84IEE75 pKa = 4.31RR76 pKa = 11.84KK77 pKa = 7.93NQRR80 pKa = 11.84TWYY83 pKa = 9.57SKK85 pKa = 9.64PRR87 pKa = 11.84CEE89 pKa = 4.79MGVTCSGRR97 pKa = 11.84QKK99 pKa = 10.65QRR101 pKa = 11.84GKK103 pKa = 10.65SIPAYY108 pKa = 10.47YY109 pKa = 10.36DD110 pKa = 2.97

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11892

27

708

180.2

20.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.258 ± 0.571

1.379 ± 0.198

5.508 ± 0.256

6.736 ± 0.525

3.305 ± 0.205

6.853 ± 0.356

1.682 ± 0.183

6.105 ± 0.241

6.248 ± 0.326

7.711 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.18

4.423 ± 0.252

3.952 ± 0.192

5.163 ± 0.399

6.307 ± 0.387

6.601 ± 0.341

5.062 ± 0.311

6.004 ± 0.296

1.564 ± 0.181

3.288 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski